Date published: 2025-9-8
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EWS Antibody (G-5) - Western Blotting - Image 355273
EWS 抗体 (G-5): sc-28327. Jurkat (A), I-11. 15 (B) 和 NRK (C) 全细胞裂解液中 EWS 表达的 Western 印迹分析.
EWS 抗体 (G-5): sc-28327. Jurkat (A), I-11. 15 (B) 和 NRK (C) 全细胞裂解液中 EWS 表达的 Western 印迹分析.

EWS 抗体 (G-5): sc-28327

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寫評論提問

说明书
  • EWS 抗体 G-5 是小鼠单克隆 IgG1 κ，EWS抗体, 在38篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的EWS的N-terminus的氨基酸2-19之间
  • EWS 抗体 (G-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的EWS; 也和以下物种反应，包括： 和 canine, bovine, porcine and avian
  • 抗EWS抗体（G-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EWS (G-5): sc-28327的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 EWS 抗体 (G-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与EWS 抗体 (G-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

EWS抗体（G-5）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的EWS蛋白。抗EWS抗体（G-5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。EWS蛋白是一种核RNA结合蛋白，在调节基因表达和RNA处理方面起着至关重要的作用。 EWS蛋白的重要性在于它参与染色体易位，导致融合蛋白的产生，如EWS/Fli-1，后者与尤因肉瘤和其他肿瘤的发病机制有关。EWS/Fli-1融合蛋白是一种强效转录激活因子，可增强靶基因的表达，从而促进细胞增殖和转化。了解EWS的功能动态及其与各种转录因子的相互作用对于阐明肿瘤发生的分子机制至关重要，这使得抗EWS抗体（G-5）成为研究癌症生物学和治疗靶点研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EWS 抗体 (G-5) 参考文献:

  1. 人类肿瘤中染色体易位引起的带有 ETS DNA 结合域的基因融合。  |  Delattre, O., et al. 1992. Nature. 359: 162-5. PMID: 1522903
  2. EWS转录激活域的分子识别。  |  Lee, KA. 2012. Adv Exp Med Biol. 725: 106-25. PMID: 22399321
  3. 通过抑制BET溴结构域靶向EWS-ETS转录程序治疗尤文氏肉瘤。  |  Hensel, T., et al. 2016. Oncotarget. 7: 1451-63. PMID: 26623725
  4. EWS-FLI1 在尤文肉瘤中增加转录物导致 R 循环并阻断 BRCA1 修复。  |  Gorthi, A., et al. 2018. Nature. 555: 387-391. PMID: 29513652
  5. TRIM8 可调节 EWS/FLI 癌症蛋白，促进尤文肉瘤患者的生存。  |  Seong, BKA., et al. 2021. Cancer Cell. 39: 1262-1278.e7. PMID: 34329586
  6. 变异型尤文氏肉瘤易位（7;22）将 EWS 基因与 ETS 基因 ETV1 融合。  |  Jeon, IS., et al. 1995. Oncogene. 10: 1229-34. PMID: 7700648
  7. 多个领域介导尤文氏肉瘤 EWS/FLI-1 融合基因的转化。  |  Lessnick, SL., et al. 1995. Oncogene. 10: 423-31. PMID: 7845667
  8. 第二种尤文氏肉瘤易位，即 t(21;22)，将 EWS 基因与另一种 ETS 家族转录因子 ERG 融合。  |  Sorensen, PH., et al. 1994. Nat Genet. 6: 146-51. PMID: 8162068
  9. 与 FLI-1 相比，尤文氏肉瘤 EWS/FLI-1 融合基因编码一种更强的转录激活剂，是一种更强大的转化基因。  |  May, WA., et al. 1993. Mol Cell Biol. 13: 7393-8. PMID: 8246959
  10. EWS-ATF-1基因涉及染色体t（12；22）易位的软组织恶性黑色素瘤，该基因编码一种组成型转录激活因子。  |  Fujimura, Y., et al. 1996. Oncogene. 12: 159-67. PMID: 8552387
  11. 尤文肉瘤染色体插入产生的 EWS-ERG 融合转录本。  |  Kaneko, Y., et al. 1997. Genes Chromosomes Cancer. 18: 228-31. PMID: 9071576
  12. 在尤文肿瘤中与 EWS 融合的 ETS 家族新成员。  |  Peter, M., et al. 1997. Oncogene. 14: 1159-64. PMID: 9121764

订购信息

产品名称产品编号规格价格数量收藏夹

EWS 抗体 (G-5)

sc-28327
200 µg/ml
RMB2377.00

EWS (G-5): m-IgG Fc BP-HRP 套装

sc-528424
200 µg Ab; 10 µg BP
RMB2662.00

EWS (G-5): m-IgGκ BP-HRP 套装

sc-520798
200 µg Ab, 40 µg BP
RMB2662.00

EWS (G-5): m-IgG1 BP-HRP 套装

sc-542925
200 µg Ab; 20 µg BP
RMB2662.00

EWS 抗体 (G-5) AC

sc-28327 AC
500 µg/ml, 25% agarose
RMB3129.00

EWS 抗体 (G-5) HRP

sc-28327 HRP
200 µg/ml
RMB2377.00

EWS 抗体 (G-5) FITC

sc-28327 FITC
200 µg/ml
RMB2482.00

EWS 抗体 (G-5) PE

sc-28327 PE
200 µg/ml
RMB2580.00

EWS 抗体 (G-5) Alexa Fluor® 488

sc-28327 AF488
200 µg/ml
RMB2685.00

EWS 抗体 (G-5) Alexa Fluor® 546

sc-28327 AF546
200 µg/ml
RMB2685.00

EWS 抗体 (G-5) Alexa Fluor® 594

sc-28327 AF594
200 µg/ml
RMB2685.00

EWS 抗体 (G-5) Alexa Fluor® 647

sc-28327 AF647
200 µg/ml
RMB2685.00

EWS 抗体 (G-5) Alexa Fluor® 680

sc-28327 AF680
200 µg/ml
RMB2685.00

EWS 抗体 (G-5) Alexa Fluor® 790

sc-28327 AF790
200 µg/ml
RMB2685.00

EWS (G-5) 中和勝肽

sc-28327 P
100 µg/0.5 ml
RMB511.00

Is the epitope of the Anti-EWS Antibody (G-5), sc-28327 in the N-terminus or internal? Your description says: "specific for an epitope mapping between amino acids 2-19 within an internal region of EWS of human origin".

Asked by: BGJo
The epitope is in the N-terminus.
Answered by: Technical Service
Date published: 2022-05-13

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-24
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Rated 5 out of 5 by from Great antibody for western blotThe batch of antibody I have worked well on BJAB, HeLa, MCF7, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 100 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 5 out of 5 by from a fantastic antibody for WBThis antibody recognizes a clear and strong signal of predicted M.W. on the western blot (1:500 dilution, 40ug HeLa extract). It also stains the nucleus as expected (1:200 dilution, formaldehyde-fixed HeLa).
Date published: 2017-09-01
Rated 5 out of 5 by from Well established cloneEWS Antibody (G-5) is a well established monoclonal antibody featured in over a dozen publications with excellent data in a variety of applications. Very popular choice!
Date published: 2017-01-06
Rated 4 out of 5 by from Various publishable data in WB using humanVarious publishable data in WB using human HEK293 cells and HeLa cells. -SCBT Publication Review
Date published: 2015-06-03
Rated 4 out of 5 by from Strong bands seen in WB using KStrong bands seen in WB using K-562 and F9 whole cell lysates and rat testis tissue extract. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Superb immunoperoxidase nuclear stainingSuperb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-02
Rated 4 out of 5 by from Publishable data in IP using human HEK293 cellsPublishable data in IP using human HEK293 cells -SCBT Publication Review
Date published: 2015-03-19
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-02-23
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EWS 抗体 (G-5) is rated 4.5 out of 5 by 8.
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