Date published: 2025-9-11
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Estrogen Receptor alpha Antibody (F-10) - Western Blotting - Image 369721
雌激素受体 α (F-10): sc-8002. MCF7 (A), T-47D (B) 和 SK-BR-3 (C) 全细胞裂解液中雌激素受体 α 表达的近红外 Western 印迹分析. 注意 C 道 (雌激素受体阴性细胞系) 无反应. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
雌激素受体 α (F-10): sc-8002. MCF7 (A), T-47D (B) 和 SK-BR-3 (C) 全细胞裂解液中雌激素受体 α 表达的近红外 Western 印迹分析. 注意 C 道 (雌激素受体阴性细胞系) 无反应. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Estrogen Receptor alpha 抗体 (F-10): sc-8002

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寫評論提問

说明书
  • Estrogen Receptor alpha 抗体 F-10 是小鼠单克隆 IgG2a κ，Estrogen Receptor alpha抗体, 在611篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的ERå的C-terminus的氨基酸576-595之间
  • Estrogen Receptor alpha 抗体 (F-10) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Estrogen Receptor alpha (F-10)
  • 还可偶联琼脂糖 用于IP; 偶联HRP 用于WB, IHC(P) 和ELISA
  • 还可偶联Alexa Fluor® 594 用于IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-8002 X, 200 µg/0.1 ml)
  • 关于如何获取Estrogen Receptor alpha (F-10): sc-8002的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Estrogen Receptor alpha Antibody (F-10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Estrogen Receptor alpha Antibody (F-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Estrogen Receptor抗体，包括Estrogen Receptor alpha, ER β, ERR α, ERR β和ERR γ

雌激素受体α抗体（F-10）是一种小鼠单克隆IgG2a kappa轻链抗体，针对人类雌激素受体α（ERα）C端576-595氨基酸之间的表位图谱。ERα单克隆抗体（F-10）可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测人体样本中的ERα。ERα是一种核激素受体，也是类固醇/甲状腺激素受体超家族的一员，它作为配体激活的转录因子，在调节包括子宫和乳腺在内的生殖组织的正常功能方面发挥着关键作用。与雌激素结合后，ERα会发生构象变化，与热休克蛋白分离，形成二聚体，并转移到细胞核，与被称为雌激素反应元件（EREs）的特定DNA序列结合，从而调节细胞生长、分化以及生殖功能所涉及的目标基因的转录。ERα的结构包括一个DNA结合域、一个配体结合域和激活域，这些结构对于与协同调节因子和转录机制的相互作用至关重要。了解ERα的功能至关重要，因为失调与多种疾病有关，特别是雌激素受体阳性乳腺癌，其中ERα促进癌细胞的增殖。ERα单克隆抗体（F-10）是研究人员在正常生理和病理条件下研究ERα的表达、定位和功能时的重要工具，有助于推进生殖生物学和癌症研究。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Estrogen Receptor alpha 抗体 (F-10) 参考文献:

  1. 维甲酸 X 受体与核受体在维甲酸, 甲状腺激素和维生素 D3 信号传导中相互作用。  |  Kliewer, SA., et al. 1992. Nature. 355: 446-9. PMID: 1310351
  2. 鉴定类固醇激素受体激活激素依赖性转录所需的保守区域。  |  Danielian, PS., et al. 1992. EMBO J. 11: 1025-33. PMID: 1372244
  3. 类固醇和甲状腺激素受体超家族。  |  Evans, RM. 1988. Science. 240: 889-95. PMID: 3283939
  4. 作为乳腺癌预后变量的孕酮和雌激素受体。  |  Mason, BH., et al. 1983. Cancer Res. 43: 2985-90. PMID: 6850609
  5. 人雌激素受体体内酪氨酸 537 和体外 src 家族酪氨酸激酶的磷酸化。  |  Arnold, SF., et al. 1995. Mol Endocrinol. 9: 24-33. PMID: 7539106
  6. ER beta：新型人类雌激素受体的鉴定和特征描述。  |  Mosselman, S., et al. 1996. FEBS Lett. 392: 49-53. PMID: 8769313
  7. 大鼠卵巢中雌激素受体-beta mRNA 的表达：促性腺激素的下调。  |  Byers, M., et al. 1997. Mol Endocrinol. 11: 172-82. PMID: 9013764
  8. 小鼠雌激素受体 beta 的克隆, 染色体定位和功能分析。  |  Tremblay, GB., et al. 1997. Mol Endocrinol. 11: 353-65. PMID: 9058381
  9. 雌激素受体 ERalpha 和 ERbeta 在 AP1 位点上的配体激活差异。  |  Paech, K., et al. 1997. Science. 277: 1508-10. PMID: 9278514

订购信息

产品名称产品编号规格价格数量收藏夹

Estrogen Receptor alpha 抗体 (F-10)

sc-8002
200 µg/ml
RMB2377.00

Estrogen Receptor alpha (F-10): m-IgG Fc BP-HRP 套装

sc-528195
200 µg Ab; 10 µg BP
RMB2662.00

Estrogen Receptor alpha (F-10): m-IgG2a BP-HRP 套装

sc-547044
200 µg Ab; 10 µg BP
RMB2662.00

Estrogen Receptor alpha (F-10): m-IgGκ BP-HRP 套装

sc-520517
200 µg Ab, 40 µg BP
RMB2662.00

Estrogen Receptor alpha 抗体 (F-10) X

sc-8002 X
200 µg/0.1 ml
RMB2377.00

Estrogen Receptor alpha 抗体 (F-10) AC

sc-8002 AC
500 µg/ml, 25% agarose
RMB3129.00

Estrogen Receptor alpha 抗体 (F-10) HRP

sc-8002 HRP
200 µg/ml
RMB2377.00

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor® 546

sc-8002 AF546
200 µg/ml
RMB2685.00

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor® 594

sc-8002 AF594
200 µg/ml
RMB2685.00

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor® 680

sc-8002 AF680
200 µg/ml
RMB2685.00

Estrogen Receptor alpha 抗体 (F-10) Alexa Fluor® 790

sc-8002 AF790
200 µg/ml
RMB2685.00

Estrogen Receptor alpha (F-10) 中和勝肽

sc-8002 P
100 µg/0.5 ml
RMB511.00

Can this antibody be used as co-ip?

Asked by: Around
Thank you for your quesiton. This antibody is not recommended to detect by CoIP.
Answered by: Technical Service SH
Date published: 2025-08-08

Does this antibody works in mouse brainstem Barrington nucleus？

Asked by: Shen PL
Thank you for your question. Estrogen Receptor alpha Antibody (F-10) is recommended for detection of Estrogen Receptor alpha (F-10) of human origin by WB, IP, IF, IHC(P) and ELISA We have the following products that are recommended for samples from mice. You can take a look. sc-8005、sc-787、sc-53493、sc-53492 and sc-71064 https://www.scbt.com/browse/estrogen-receptor-alpha-antibodies
Answered by: Ling Chen
Date published: 2025-02-10

我之前在国外用这个抗体，感觉非常好用。现在回国后又购买了该抗体，却无法染出来。不知道是否有批次效应？可否请求发送一个新的试用装，谢谢！

Asked by: zz_fudan
感谢您的提问。您可以拨打电话：021-60936350，发送邮件到[email protected]，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2023-08-29

I used this antibody to stain every step of a pull down performed with this antibody.. I see two bands in the pull down - one around 55 kDa and one around 25 kDa. What could the lower one be? - Normal HeLa cells were used. Thanks

Asked by: Danielle17
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-06-15

请问到广东深圳的货期是多久？

Asked by: Anonymous
感谢您的提问。如果你能直接给我们发邮件到[email protected]，那将会很有帮助。这样我们可以就这个问题和其他相关问题进行更互动的讨论。 Thank you for your question. It would be helpful if you could email us directly at [email protected]. So we can have a more interactive discussion about this and other related issues.
Answered by: Zhanglong
Date published: 2022-06-08

国内有现货吗 邮寄到合肥大致需要多久

Asked by: yang xx
感谢您的提问。请联系我们的技术支持部门了解更多信息。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2021-12-21

come posso avere sample gratuiti di prova?

Asked by: aldo Mileo
Grazie per la tua domanda. Si prega di contattare direttamente il nostro team di assistenza tecnica per organizzare un campione gratuito.
Answered by: Tech Support Europe
Date published: 2021-07-23

What amino acids does this antibody target on ERa? Does it cover the AF1 domain and the AF2 (Ligand binding domain)? Or does it just cover the AF2 Domain (Ligand Binding Domain)?

Asked by: JohnP
Please contact Technical Service for binding site inquiries.
Answered by: Tech Sales
Date published: 2021-05-12
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Rated 1 out of 5 by from Even if antigen retrieval is done, it doesn't workEven if antigen retrieval is done, it doesn't work.
Date published: 2023-09-02
Rated 4 out of 5 by from Good AntibodyI tested this antibody for the Western Blot and the signals appears good in the breast cancer cells as indicated in the datasheet.
Date published: 2023-07-12
Rated 4 out of 5 by from Weird signals but I suppose it worksThe band looks great in the input but during a pulldown there is a huge smear at a higher MW. Potentially it does not work on pulldowns? I've stained the same blot using antibodies from other vendor for ERa and it worked very well.
Date published: 2022-10-21
Rated 4 out of 5 by from Ok, worked fineIt worked fine in western blot at the suggested dilution of 1:200 in human samples.
Date published: 2021-12-21
Rated 5 out of 5 by from Better than other antibodiesI used C-311 at the same time, but F-10 was better.
Date published: 2021-06-18
Rated 3 out of 5 by from Not working.Tried free sample. Got some bands but not the right size.
Date published: 2020-10-08
Rated 5 out of 5 by from Best one!!!I used this antibody for our ER alpha detection in human mammary epithelial cell line and it gave good nuclear staining. Totally satisfied. Thank you.
Date published: 2020-06-22
Rated 4 out of 5 by from Good productI got a free sample of this antibody. It works good.
Date published: 2019-08-13
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Estrogen Receptor alpha 抗体 (F-10) is rated 4.6 out of 5 by 54.
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