Date published: 2025-9-7
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eIF4E Antibody (A-10) - Western Blotting - Image 353962
eIF4E 抗体 (A-10): sc-271480. 对 NCI-H460 全细胞裂解液 (A) 和人胎肝 (B), 小鼠胃 (C) 和大鼠肝 (D) 组织提取物中 eIF4E 的表达进行 Western 印迹分析.
eIF4E 抗体 (A-10): sc-271480. 对 NCI-H460 全细胞裂解液 (A) 和人胎肝 (B), 小鼠胃 (C) 和大鼠肝 (D) 组织提取物中 eIF4E 的表达进行 Western 印迹分析.

eIF4E 抗体 (A-10): sc-271480

5.0(10)
寫評論提問

说明书
  • eIF4E抗体(A-10)是小鼠单克隆IgG2b κ, 在37篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长eIF4E的氨基酸1-217
  • 抗-eIF4E 抗体 (A-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 eIF4E
  • 抗eIF4E抗体（A-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取eIF4E (A-10): sc-271480的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是eIF4E 抗体 (A-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与eIF4E 抗体 (A-10) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 eIF 抗体 包括 eIF1, eIF1AY, eIF2 α, eIF2B α, eIF2B β, eIF2B δ, eIF2B ε, eIF2 β, eIF2B γ和eIF2C

eIF4E抗体（A-10）是一种IgG2b κ小鼠单克隆eIF4E抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的eIF4E。eIF4E抗体（A-10）有非偶联抗eIF4E抗体形式，以及多种偶联形式的抗eIF4E抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。真核细胞中蛋白质合成的起始由蛋白质起始因子和RNA分子之间的相互作用调节。真核起始复合物eIF4F在体外以eIF4E、eIF4A和eIF4G的三聚体复合物存在。该复合物通过诱导mRNA二级结构的解旋使核糖体结合mRNA。在蛋白质合成的早期步骤中，eIF4E与mRNA"帽"结合。eIF4A作为ATP依赖的RNA解旋酶。eIF4G作为eIF4E、eIF4A和eIF3复合物之间的桥梁。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

eIF4E 抗体 (A-10) 参考文献:

  1. 翻译因子eIF4E在细胞核中发挥的新作用。  |  Strudwick, S. and Borden, KL. 2002. Differentiation. 70: 10-22. PMID: 11963652
  2. eIF4E--从翻译到转化。  |  Mamane, Y., et al. 2004. Oncogene. 23: 3172-9. PMID: 15094766
  3. eIF4E，mRNA帽结合蛋白：从基础发现到转化研究。  |  Sonenberg, N. 2008. Biochem Cell Biol. 86: 178-83. PMID: 18443631
  4. 翻译起始因子eIF4E的结构和功能及其在癌症发展和治疗中的作用。  |  Piserà, A., et al. 2018. J Genet Genomics. 45: 13-24. PMID: 29396141
  5. 真核生物转化起始因子 eIF4E 的生化和结构透视  |  Volpon, L., et al. 2019. Curr Protein Pept Sci. 20: 525-535. PMID: 30636602
  6. 监测eIF4E依赖性核3'端mRNA切割。  |  Delaleau, M. 2021. Methods Mol Biol. 2209: 347-361. PMID: 33201480
  7. eIF4E与eIF4E相互作用蛋白之间的多种关系。  |  Hernández, G. 2022. Trends Genet. 38: 801-804. PMID: 35568601
  8. miR-34b-3p抑制eIF4E导致成年小鼠中风后抑郁。  |  Ke, X., et al. 2023. Neurosci Bull. 39: 194-212. PMID: 35802246
  9. 真核翻译起始因子 eIF4E 重编程替代剪接。  |  Ghram, M., et al. 2023. EMBO J. 42: e110496. PMID: 36843541
  10. 磷酸化eIF4E刺激通过选择性表达细胞膜驻留因子来调节冠状病毒的进入。  |  Zhang, X-Z., et al. 2024. J Virol. 98: e0194823. PMID: 38299843

订购信息

产品名称产品编号规格价格数量收藏夹

eIF4E 抗体 (A-10)

sc-271480
200 µg/ml
RMB2377.00

eIF4E (A-10): m-IgG2b BP-HRP 套装

sc-548702
200 µg Ab; 10 µg BP
RMB2662.00

eIF4E 抗体 (A-10) AC

sc-271480 AC
500 µg/ml, 25% agarose
RMB3129.00

eIF4E 抗体 (A-10) HRP

sc-271480 HRP
200 µg/ml
RMB2377.00

eIF4E 抗体 (A-10) FITC

sc-271480 FITC
200 µg/ml
RMB2482.00

eIF4E 抗体 (A-10) PE

sc-271480 PE
200 µg/ml
RMB2580.00

eIF4E 抗体 (A-10) Alexa Fluor® 488

sc-271480 AF488
200 µg/ml
RMB2685.00

eIF4E 抗体 (A-10) Alexa Fluor® 546

sc-271480 AF546
200 µg/ml
RMB2685.00

eIF4E 抗体 (A-10) Alexa Fluor® 594

sc-271480 AF594
200 µg/ml
RMB2685.00

eIF4E 抗体 (A-10) Alexa Fluor® 647

sc-271480 AF647
200 µg/ml
RMB2685.00

eIF4E 抗体 (A-10) Alexa Fluor® 680

sc-271480 AF680
200 µg/ml
RMB2685.00

eIF4E 抗体 (A-10) Alexa Fluor® 790

sc-271480 AF790
200 µg/ml
RMB2685.00

I bought this antibody recently for IP, but I couldn't get any pulldown. But within the same experiment, I have success pulldown of the other protein. Is there specific detergent the antibody might not be compatible with?

Asked by: ihsun
Thank you for your question. This mouse monoclonal, eIF4E Antibody (A-10): sc-271480, has been published for use in IP research applications. Please refer to the following publications on PubMed for more information about the specific methods used: PMID's: 26370510 & 27053525. In addition, a link to our recommended protocol for IP is provided below. https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots If you continue to have trouble using the antibody, please contact our Technical Service Department for help with troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-04-26

Can you recommend a positive control to use with eIF4E Antibody (A-10): sc-271480 for Western blot?

Asked by: jenni
We recommend KNRK (sc-2214), K-562 (sc-2203) or MCF7 (sc-2206) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of eIF4E in these cell lines.
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 5 out of 5 by from Signal detected on mouse tumor sectionsAntibody works well on parafilm sections of mouse mammary tumors. Provided a clear signal
Date published: 2018-04-30
Rated 5 out of 5 by from good antibody for WBthe data looks good and the background is clean. I would like to recommend this antibody.
Date published: 2017-07-03
Rated 5 out of 5 by from Excellent AbI bought this antibody a month ago. It works very well for IP and WB.
Date published: 2017-03-14
Rated 5 out of 5 by from Great antibodyStrong antibody for multiple applications including western blot, IHC and IF
Date published: 2017-01-10
Rated 5 out of 5 by from Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Very versatile.
Date published: 2016-12-29
Rated 5 out of 5 by from Works very wellWorks very well. -SCBT Publication Review
Date published: 2016-04-14
Rated 5 out of 5 by from Worked well in WB in KNRK and SRCWorked well in WB in KNRK and SRC-3T3 whole cell lysates. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Strong cytoplasmic staining of neuronal andStrong cytoplasmic staining of neuronal and glial cells in IHC of human brain tissue. -SCBT QC
Date published: 2015-02-20
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eIF4E 抗体 (A-10) is rated 5.0 out of 5 by 10.
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