Date published: 2025-9-7

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eIF4E 抗体 (P-2): sc-9976

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说明书
  • eIF4E 抗体 P-2 是小鼠单克隆 IgG1 κ,eIF4E抗体, 在136篇文献中引用,规格为200 µg/ml
  • 针对全长eIF4E,human起源
  • eIF4E 抗体 (P-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat, human 和porcine 来源的 eIF4E
  • 抗eIF4E抗体(P-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取eIF4E (P-2): sc-9976免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 eIF4E 抗体 (P-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与eIF4E 抗体 (P-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

eIF4E 抗体(P-2)是一种鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测鼠、大鼠、人和猪源的 eIF4E。抗eIF4E抗体(P-2)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素( )等多种Alexa Fluor®偶联物。eIF4E在真核细胞蛋白质合成的起始阶段起着至关重要的作用,因为它与mRNA的5'帽结合,促进核糖体与mRNA转录本的结合。这种相互作用对于将mRNA正确翻译成蛋白质至关重要,而蛋白质对于许多细胞功能和过程至关重要。eIF4E是eIF4F复合物的一部分,该复合物还包括eIF4A和eIF4G;这些蛋白质共同作用,使mRNA二级结构解开,从而实现高效的核糖体结合。调节eIF4E的活性至关重要,因为失调会导致包括癌症在内的多种疾病,因此eIF4E成为治疗干预和研究的重要靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

eIF4E 抗体 (P-2) 参考文献:

  1. 人类蛋白质合成启动因子 eIF-4 gamma 的氨基酸序列。  |  Yan, R., et al. 1992. J Biol Chem. 267: 23226-31. PMID: 1429670
  2. 真核生物引发剂-4E 和-4F 既能引发蛋白质合成的 5'帽子依赖型启动,也能引发蛋白质合成的内部启动。  |  Scheper, GC., et al. 1992. J Biol Chem. 267: 7269-74. PMID: 1559971
  3. 翻译中的 RNA 解旋:由真核生物引发剂 eIF-4F 和 eIF-4B 组成的螺旋酶复合体中间体的组装。  |  Jaramillo, M., et al. 1991. Mol Cell Biol. 11: 5992-7. PMID: 1719376
  4. 真核生物翻译起始因子 4A 和 4F 的双向 RNA 螺旋酶活性。  |  Rozen, F., et al. 1990. Mol Cell Biol. 10: 1134-44. PMID: 2304461
  5. eIF4E抗性:自然变异应指导基因编辑。  |  Bastet, A., et al. 2017. Trends Plant Sci. 22: 411-419. PMID: 28258958
  6. 蛋白质合成起始因子 4A 基因的分离和图谱绘制及其在小鼠红细胞白血病细胞分化过程中的表达。  |  Reddy, NS., et al. 1988. Gene. 70: 231-43. PMID: 3215517
  7. 人体组织中 mRNA 盖帽结合蛋白的氨基酸序列。  |  Rychlik, W., et al. 1987. Proc Natl Acad Sci U S A. 84: 945-9. PMID: 3469651
  8. eIF4E同源蛋白(4EHP):一种多种帽子结合蛋白  |  Christie, M. and Igreja, C. 2023. FEBS J. 290: 266-285. PMID: 34758096
  9. eIF4E整合到应激反应中。  |  Wu, X. and Qian, SB. 2024. Mol Cell. 84: 2009-2010. PMID: 38848688
  10. eIF4E-独立翻译在很大程度上依赖于eIF3d。  |  Roiuk, M., et al. 2024. Nat Commun. 15: 6692. PMID: 39107322
  11. 用皮卡病毒蛋白酶绘制真核蛋白质合成起始因子 4G(eIF4G)的功能域图。帽子依赖型和帽子非依赖型翻译启动的意义。  |  Lamphear, BJ., et al. 1995. J Biol Chem. 270: 21975-83. PMID: 7665619
  12. 真核蛋白质合成:体外分析。  |  Merrick, WC. 1994. Biochimie. 76: 822-30. PMID: 7880898

订购信息

产品名称产品编号规格价格数量收藏夹

eIF4E 抗体 (P-2)

sc-9976
200 µg/ml
$316.00

eIF4E (P-2): m-IgG Fc BP-HRP 套装

sc-528244
200 µg Ab; 10 µg BP
$354.00

eIF4E (P-2): m-IgGκ BP-HRP 套装

sc-520571
200 µg Ab, 40 µg BP
$354.00

eIF4E (P-2): m-IgG1 BP-HRP 套装

sc-542834
200 µg Ab; 20 µg BP
$354.00

eIF4E 抗体 (P-2) AC

sc-9976 AC
500 µg/ml, 25% agarose
$416.00

eIF4E 抗体 (P-2) HRP

sc-9976 HRP
200 µg/ml
$316.00

eIF4E 抗体 (P-2) FITC

sc-9976 FITC
200 µg/ml
$330.00

eIF4E 抗体 (P-2) PE

sc-9976 PE
200 µg/ml
$343.00

eIF4E 抗体 (P-2) Alexa Fluor® 488

sc-9976 AF488
200 µg/ml
$357.00

eIF4E 抗体 (P-2) Alexa Fluor® 546

sc-9976 AF546
200 µg/ml
$357.00

eIF4E 抗体 (P-2) Alexa Fluor® 594

sc-9976 AF594
200 µg/ml
$357.00

eIF4E 抗体 (P-2) Alexa Fluor® 647

sc-9976 AF647
200 µg/ml
$357.00

eIF4E 抗体 (P-2) Alexa Fluor® 680

sc-9976 AF680
200 µg/ml
$357.00

eIF4E 抗体 (P-2) Alexa Fluor® 790

sc-9976 AF790
200 µg/ml
$357.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 5 out of 5 by from Great antibody for westernI bought this as a free sample and it is works perfectly fine for western
Date published: 2022-09-02
Rated 3 out of 5 by from Some backroundThe bands were specific and at the correct size, but there was a bit background on the film.
Date published: 2021-03-17
Rated 5 out of 5 by from Sharp, crisp bands at expected sizeGreat, specific bands with no noticeable background
Date published: 2020-07-03
Rated 5 out of 5 by from Works wellI bought a free sample from santa cruz and it works well for WB
Date published: 2019-11-21
Rated 4 out of 5 by from Buon funzionamento!Ho acquistato e testato un campione di prova diverse settimane fa, ottenendo un buon risultato con una concentrazione di anticorpo ottimale per i consumi.
Date published: 2019-07-23
Rated 5 out of 5 by from ExcellentWorks well with 1:500 dilution. Very strong band at designed MW. No non-specific bands.
Date published: 2018-10-10
Rated 3 out of 5 by from Signal detected for mouse mammary tumorsSpecific signal was detected on parafilm sections of mouse mammary tumors.
Date published: 2018-04-30
Rated 5 out of 5 by from quite strong band for wb of rat protein1:1000 diluted for wb of rat heart protein,the band is quite strong!
Date published: 2017-11-05
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eIF4E 抗体 (P-2) is rated 4.7 out of 5 by 23.
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