Date published: 2025-9-7

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DEC2 抗体 (E-4): sc-373763

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说明书
  • DEC2 抗体 E-4 是小鼠单克隆 IgG1 κ,DEC2抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列411-482位于human物种的DEC2的C-terminus
  • DEC2 抗体 (E-4) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 DEC2
  • 抗DEC2抗体(E-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-373763 X, 200 µg/0.1 ml)
  • 关于如何获取DEC2 (E-4): sc-373763免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是DEC2 Antibody (E-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与DEC2 Antibody (E-4) 打包提供(请参阅下面的订购信息)。

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関連項目

DEC2抗体(E-4)是一种IgG1 κ小鼠单克隆DEC2抗体(也称为DEC2抗体),它通过WB、IP、IF和ELISA检测人类的DEC2蛋白。DEC2抗体(E-4)既可作为非偶联抗DEC2抗体形式,也可作为多种偶联形式的抗DEC2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。人类DEC1是一种含有412个氨基酸、基本螺旋-环-螺旋(bHLH)的蛋白质,参与控制多种细胞类型(包括神经细胞、成纤维细胞和软骨细胞)的增殖和/或分化。DEC1的bHLH区域在结构上与哺乳动物HES家族、果蝇hairy和果蝇Enhancer of split m7的bHLH区域相似。DEC1是cAMP在多种细胞中的新型直接靶标,参与cAMP激活细胞中基因表达的控制。DEC2,也称为SHARP1,在骨骼肌和大脑中高度表达。编码人类DEC2的基因定位于染色体12p11.23-p12.1。DEC1和DEC2通过抑制特定时钟基因的转录在调节哺乳动物分子时钟中发挥作用。DEC1和DEC2均以昼夜节律的方式在视交叉上核中被检测到。短暂的光脉冲以相位依赖的方式诱导DEC1的表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DEC2 抗体 (E-4) 参考文献:

  1. 基本螺旋-环-螺旋蛋白新成员 DEC2 的分子克隆和特征描述。  |  Fujimoto, K., et al. 2001. Biochem Biophys Res Commun. 280: 164-71. PMID: 11162494
  2. 基本螺旋环螺旋蛋白 DEC1 (BHLHB2)/Stra13/Sharp2 对环磷酸腺苷通路的诱导。  |  Shen, M., et al. 2001. Eur J Cell Biol. 80: 329-34. PMID: 11432722
  3. Dec1 和 Dec2 是哺乳动物分子钟的调节器。  |  Honma, S., et al. 2002. Nature. 419: 841-4. PMID: 12397359
  4. DEC1/STRA13/SHARP2和DEC2/SHARP1协调生理过程,包括对环境刺激的反应的昼夜节律。  |  Kato, Y., et al. 2014. Curr Top Dev Biol. 110: 339-72. PMID: 25248482
  5. DEC2表达与HIF-1激活和人类骨肉瘤的侵袭性呈正相关。  |  Hu, T., et al. 2015. J Exp Clin Cancer Res. 34: 22. PMID: 25884381
  6. DEC1是口腔鳞状细胞癌早期转移的潜在标志物。  |  Sato, F., et al. 2016. J Cancer. 7: 153-9. PMID: 26819638
  7. DEC2 阻断 ARNTL2/NPAS2 二聚体对 PER3 和 DBP 表达的影响。  |  Olkkonen, J., et al. 2017. J Circadian Rhythms. 15: 6. PMID: 30210560
  8. Dec2可减弱发炎牙周组织中的自噬作用。  |  Oka, S., et al. 2021. Immun Inflamm Dis. 9: 265-273. PMID: 33270996
  9. Dec2 可抑制巨噬细胞的脓毒症,促进牙周稳态。  |  He, D., et al. 2022. J Periodontal Implant Sci. 52: 28-38. PMID: 35187871
  10. Dec2在牙周炎中负调节骨吸收。  |  Li, X., et al. 2022. J Periodontal Res. 57: 1056-1069. PMID: 35989621
  11. BHLHE41/DEC2在非小细胞肺癌发展中的作用。  |  Furukawa, T., et al. 2023. Int J Mol Sci. 24: PMID: 37511489
  12. 分化的人类胚胎软骨细胞中表达的新型碱性螺旋环螺旋蛋白 DEC1 的分子特征。  |  Shen, M., et al. 1997. Biochem Biophys Res Commun. 236: 294-8. PMID: 9240428

订购信息

产品名称产品编号规格价格数量收藏夹

DEC2 抗体 (E-4)

sc-373763
200 µg/ml
RMB2377.00

DEC2 (E-4): m-IgG Fc BP-HRP 套装

sc-529554
200 µg Ab; 10 µg BP
RMB2662.00

DEC2 (E-4): m-IgGκ BP-HRP 套装

sc-522487
200 µg Ab, 40 µg BP
RMB2662.00

DEC2 (E-4): m-IgG1 BP-HRP 套装

sc-543577
200 µg Ab; 20 µg BP
RMB2662.00

DEC2 抗体 (E-4) X

sc-373763 X
200 µg/0.1 ml
RMB2377.00

DEC2 抗体 (E-4) AC

sc-373763 AC
500 µg/ml, 25% agarose
RMB3129.00

DEC2 抗体 (E-4) HRP

sc-373763 HRP
200 µg/ml
RMB2377.00

DEC2 抗体 (E-4) FITC

sc-373763 FITC
200 µg/ml
RMB2482.00

DEC2 抗体 (E-4) PE

sc-373763 PE
200 µg/ml
RMB2580.00

DEC2 抗体 (E-4) Alexa Fluor® 488

sc-373763 AF488
200 µg/ml
RMB2685.00

DEC2 抗体 (E-4) Alexa Fluor® 546

sc-373763 AF546
200 µg/ml
RMB2685.00

DEC2 抗体 (E-4) Alexa Fluor® 594

sc-373763 AF594
200 µg/ml
RMB2685.00

DEC2 抗体 (E-4) Alexa Fluor® 647

sc-373763 AF647
200 µg/ml
RMB2685.00

DEC2 抗体 (E-4) Alexa Fluor® 680

sc-373763 AF680
200 µg/ml
RMB2685.00

DEC2 抗体 (E-4) Alexa Fluor® 790

sc-373763 AF790
200 µg/ml
RMB2685.00

Why the wb experiment with SH-SY2Y cells showed that the position of dec2 was around 80kd,

Asked by: Anonymous
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2023-06-01

What is the recommended starting dilution for Western blot for sc-373763: DEC2 (E-4) monoclonal antibody?

Asked by: Trav11
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from it doesn't work on my samples!It doesn't work well on cellular lysates (HEK293T and MDA231) both by using western blot (1:500) and IP (dilution 1:200).
Date published: 2023-06-27
Rated 4 out of 5 by from Good antibodyWorks very good. Sharp bands at 48kDa. Recommended to use
Date published: 2018-06-19
Rated 5 out of 5 by from Great Western Blot BandWe used this antibody for western blot and got a perfect band for the correct molecular weight
Date published: 2017-01-17
Rated 5 out of 5 by from Produced positive Western blot data of DEC2Produced positive Western blot data of DEC2 expression in SH-SY5Y and IMR-32 nuclear extracts. -SCBT QC
Date published: 2014-02-03
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DEC2 抗体 (E-4) is rated 3.8 out of 5 by 4.
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