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DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6) - Western Blotting - Image 109575
DAT 抗体 (6-8D6): sc-32259. SH-SY5Y (A) 和 SK-N-SH (B) 全细胞裂解液中 DAT 表达的 Western 印迹分析.
DAT 抗体 (6-8D6): sc-32259. SH-SY5Y (A) 和 SK-N-SH (B) 全细胞裂解液中 DAT 表达的 Western 印迹分析.

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6): sc-32259

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寫評論提問

说明书
  • DAT/Dopamine Transporter/SLC6A3 抗体 6-8D6 是大鼠单克隆 IgG2a，DAT/Dopamine Transporter/SLC6A3抗体, 在20篇文献中引用，规格为200 µg/ml
  • 针对氨基酸180-218的抗性，对应DAT的第二个细胞外环，human起源
  • DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 DAT
  • 抗DAT/Dopamine Transporter/SLC6A3抗体（6-8D6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DAT/Dopamine Transporter/SLC6A3 (6-8D6): sc-32259的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

背景

DAT抗体（6-8D6）是一种鼠单克隆IgG2a抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的DAT蛋白。抗DAT抗体（6-8D6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。多巴胺转运蛋白（DAT）通过促进多巴胺从细胞外空间重新摄取回突触前神经元，在调节突触间隙的多巴胺水平方面发挥着至关重要的作用。这一功能对于维持多巴胺的稳态至关重要，而多巴胺稳态对于正常的神经功能和行为必不可少。DAT活性的失调与多种神经精神疾病有关，包括注意力缺陷多动障碍（ADHD）和药物滥用障碍，这凸显了DAT在健康和疾病中的重要性。DAT与多种神经毒素的相互作用凸显了其在神经退行性疾病中的重要性，尤其是在帕金森病中，DAT功能障碍会导致多巴胺能神经元的选择性脆弱性。

染色质位置

基因座：SLC6A3（人类）定位于5p15.33；Slc6a3（小鼠）定位于13 C1。

来源

DAT (6-8D6) 是一种鼠单克隆抗体，针对与人类DAT的第二个细胞外环对应的氨基酸180-218。

产品

每瓶含200 µg IgG2a，1.0 ml PBS，< 0.1% 叠氮化钠和0.1% 明胶。

  • 抗DAT/Dopamine Transporter/SLC6A3抗体（6-8D6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

应用

建议使用DAT (6-8D6)通过Western Blotting（起始稀释度为1:200，稀释度范围为1:100-1:1000）、免疫沉淀（每100-500 µg总蛋白（1 ml细胞裂解液））、免疫荧光（起始稀释度1:50，稀释度范围1:50-1:500）和免疫组织化学（包括石蜡包埋切片）（起始稀释度1:50，稀释度范围1:50-1:500）。

非糖基化 DAT 的分子量：50 kDa

糖基化 DAT 的分子量：80 kDa

保存

在 4°C 下保存，**请勿冷冻**。自发货之日起一年内稳定。非危险品。无需 MSDS。

研究用途

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6)

sc-32259
200 µg/ml
RMB2377.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) AC

sc-32259 AC
500 µg/ml, 25% agarose
RMB3129.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) HRP

sc-32259 HRP
200 µg/ml
RMB2377.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) FITC

sc-32259 FITC
200 µg/ml
RMB2482.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) PE

sc-32259 PE
200 µg/ml
RMB2580.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 488

sc-32259 AF488
200 µg/ml
RMB2685.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 546

sc-32259 AF546
200 µg/ml
RMB2685.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 594

sc-32259 AF594
200 µg/ml
RMB2685.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 647

sc-32259 AF647
200 µg/ml
RMB2685.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 680

sc-32259 AF680
200 µg/ml
RMB2685.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 790

sc-32259 AF790
200 µg/ml
RMB2685.00

Are there reports using this DAT ab for Immunoprecipitation, if so which ones? Could it be used for magnetic isolation by coupling to Dynabeads?

Asked by: andresj1
Thank you for your question. DAT Antibody (6-8D6): sc-32259 has been tested and is recommended for IP applications with DAT of mouse, rat and human origin. As of now there are no publications in which this antibody has been used for immunoprecipitation.
Answered by: Tech Support Europe
Date published: 2020-07-14

Can I use it on rat sections, blocking with normal donkey serum?

Asked by: ann1
Thank you for your question. Yes, this antibody is mouse, rat and human reactive, so using it on rat samples is fine. If you are doing a Western blot we would recommend blocking in 5% milk with TBS-T. You can block in normal donkey serum if you are doing an IHC or IF, especially if you are using a donkey anti-rat secondary antibody. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-11-06

Is a blocking peptide for with this monoclonal antibody available?

Asked by: AbPolly
Thank you for your question. This DAT antibody sc-32259 is raised against a recombinant protein and not a short synthetic peptide and a blocking peptide is not possible to design for it.
Answered by: Technical Support 7
Date published: 2017-05-16
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Rated 5 out of 5 by from Works well for mouse brain tissuePurchased for IHC of mouse brain slices fixed in 0.4% PFA. Used 1:50 down to 1:4000 dilutions (in 4% BSA with 0.3% Triton-X100) and saw great staining of straitum.
Date published: 2021-07-15
Rated 4 out of 5 by from great antibodyI acquired the antibody a while ago. Works greatly and as good as other DATmAb from other vendors. We chose to purchase after sampling the product.
Date published: 2019-03-22
Rated 3 out of 5 by from Good!I applied this antibody last year. This antibody couldn't use with rats. It's very sad.
Date published: 2018-03-02
Rated 5 out of 5 by from 功能很出色上半年申请的该抗体，用于westernblot实验，样品为N2A细胞，上样量为50 ug，曝光结果较好。
Date published: 2017-09-27
Rated 5 out of 5 by from ClearnessI used the antibody in WB at 1:300, PC12 cells. The protein band was clear, and there was no other bands. The pictures shows that the protein band is a little narrow, possibly because I used the 10% SDS separation gel.
Date published: 2017-09-18
Rated 5 out of 5 by from thisAdrenergic binding of the beta - adrenergic receptor activates adenylate cyclase, whereas adrenergic binding of the α2 - adrenergic receptor inhibits adenylate cyclase. The action of the molecule can be excitatory or inhibitory, depending on the type of receptor to which it is bound.
Date published: 2017-02-08
Rated 5 out of 5 by from DAT (6-8D6) AntibodyWorks great for Western blot (WB), band at 80 kDa. We have not characterized the band above 90 kDa.
Date published: 2017-01-05
Rated 4 out of 5 by from Pretty good...Used in IPs and WB and it worked as expected. Stable for years when stored correctly.
Date published: 2016-12-05
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DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) is rated 4.5 out of 5 by 11.
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