Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
DAT/Dopamine Transporter/SLC6A3 Antibody (6-5G10) - Western Blotting - Image 145761
DAT 抗体 (6-5G10): sc-32258. 人尾状脑组织提取物中 DAT 表达的 Western 印迹分析.
DAT 抗体 (6-5G10): sc-32258. 人尾状脑组织提取物中 DAT 表达的 Western 印迹分析.

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10): sc-32258

4.2(13)
寫評論提問

说明书
  • DAT/Dopamine Transporter/SLC6A3 抗体 6-5G10 是大鼠单克隆 IgG, 在58篇文献中引用，提供 200 µg/ml
  • 针对重组融合蛋白DAT的抗原决定簇1-66
  • 抗-DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 DAT
  • 抗DAT/Dopamine Transporter/SLC6A3抗体（6-5G10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DAT/Dopamine Transporter/SLC6A3 (6-5G10): sc-32258的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

DAT抗体（6-5G10）是一种鼠单克隆IgG抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类样本中的DAT（多巴胺转运蛋白）。抗DAT抗体（6-5G10）有非结合型和结合型两种形式，可与琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®染料结合。多巴胺转运蛋白（DAT）是溶质载体家族6的成员，通过促进多巴胺从细胞外空间重新摄取到突触前神经元中，从而调节突触间隙中的多巴胺水平。这一功能维持多巴胺能信号，对情绪调节、奖励和运动控制至关重要。DAT功能障碍与多种神经精神疾病有关，包括抑郁症、精神分裂症和注意力缺陷多动障碍（ADHD），这凸显了DAT在健康和疾病中的重要性。此外，DAT能够运输神经毒素，这会导致多巴胺能神经元在帕金森病等疾病中变得脆弱。研究DAT的动态和蛋白质相互作用可能会为这些疾病提供新的治疗方法。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) 参考文献:

  1. 真核细胞中表达的α-肾上腺素能受体亚型的多种第二信使途径。  |  Cotecchia, S., et al. 1990. J Biol Chem. 265: 63-9. PMID: 2152928
  2. 多巴胺转运蛋白（DAT）扫描在运动障碍中心的应用。  |  Oravivattanakul, S., et al. 2016. Mov Disord Clin Pract. 3: 31-35. PMID: 30363515
  3. 多模式多巴胺转运蛋白（DAT）成像和磁共振成像（MRI）对早期帕金森病的表征。  |  Porter, E., et al. 2020. Parkinsonism Relat Disord. 79: 26-33. PMID: 32861103
  4. 帕金森病及相关疾病中的多巴胺转运蛋白（DAT）成像。  |  Brücke, T. and Brücke, C. 2022. J Neural Transm (Vienna). 129: 581-594. PMID: 34910248
  5. 通过CRISPR-Cas9基因组编辑产生多巴胺转运蛋白（DAT）-mCherry敲入小鼠。  |  Matsumoto, N., et al. 2024. Biol Pharm Bull. 47: 394-398. PMID: 38325828
  6. 利培酮可降低精神分裂症患者PBMC中5-羟色胺受体2A（5-HT2A）和5-羟色胺转运体（SERT）的表达，但不影响多巴胺受体和多巴胺转运体（DAT）的表达。  |  Alvarez-Herrera, S., et al. 2024. Pharmaceuticals (Basel). 17: PMID: 38399382
  7. 通过多巴胺转运蛋白（DAT）-Cre报告基因小鼠对肠神经元进行表征，揭示了具有双重递质含量的多巴胺能亚型。  |  Recinto, SJ., et al. 2024. Eur J Neurosci. 59: 2465-2482. PMID: 38487941
  8. 激动剂诱导的多巴胺 D1 受体刺激腺苷酸环化酶活性脱敏与 D1 受体内化在时间和生物化学上是分离的。  |  Ng, GY., et al. 1995. Proc Natl Acad Sci U S A. 92: 10157-61. PMID: 7479745
  9. G 蛋白偶联受体超家族中保守的七跨膜序列 NP(X)2,3Y 调节着 beta 2-肾上腺素能受体的多种特性。  |  Barak, LS., et al. 1995. Biochemistry. 34: 15407-14. PMID: 7492540
  10. 多巴胺受体亚型的分子和化学神经药理学。  |  Ogawa, N. 1995. Acta Med Okayama. 49: 1-11. PMID: 7762403
  11. D2 多巴胺受体异构体通过不同的 Gi alpha 蛋白发出信号以抑制腺苷酸环化酶。利用定点突变的 Gi alpha 蛋白进行的研究。  |  Senogles, SE. 1994. J Biol Chem. 269: 23120-7. PMID: 7916015
  12. 多巴胺转运体定域于黑质多巴胺能神经元的树突和轴突浆膜。  |  Nirenberg, MJ., et al. 1996. J Neurosci. 16: 436-47. PMID: 8551328

订购信息

产品名称产品编号规格价格数量收藏夹

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10)

sc-32258
200 µg/ml
RMB2377.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) AC

sc-32258 AC
500 µg/ml, 25% agarose
RMB3129.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) HRP

sc-32258 HRP
200 µg/ml
RMB2377.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) FITC

sc-32258 FITC
200 µg/ml
RMB2482.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) PE

sc-32258 PE
200 µg/ml
RMB2580.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) Alexa Fluor® 488

sc-32258 AF488
200 µg/ml
RMB2685.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) Alexa Fluor® 546

sc-32258 AF546
200 µg/ml
RMB2685.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) Alexa Fluor® 594

sc-32258 AF594
200 µg/ml
RMB2685.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) Alexa Fluor® 647

sc-32258 AF647
200 µg/ml
RMB2685.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) Alexa Fluor® 680

sc-32258 AF680
200 µg/ml
RMB2685.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) Alexa Fluor® 790

sc-32258 AF790
200 µg/ml
RMB2685.00

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 9
Date published: 2017-01-26
  • y_2025, m_8, d_31, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_32258, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 94ms
  • QUESTIONS, PRODUCT
Rated 3 out of 5 by from Chronic exposure to cannabinoidsChronic exposure to cannabinoids before an emotional trauma may have negative effects on emotional function
Date published: 2021-10-18
Rated 4 out of 5 by from Quite good in IFI used it in IF on hiPSC-derived dopaminergic neurons, at day 45 of in vitro differentiation. Fixation with 4% PFA for 15'; permeabilization with 0,3% triton for 15'; blocking with 3% BSA, 2% donkey serum, 0,1% tween for 1h and 1/2. I used a 1:100 dilution: the signal was good in cells that were highly expressing DAT, whereas in cells with lower DAT expression the signal was low and with some background.
Date published: 2019-03-18
Rated 5 out of 5 by from Working well for WBI used this antibody for western blot with rat striatal tissue, and it works well.
Date published: 2018-05-06
Rated 3 out of 5 by from Works for IHC but in high concentrationTried this antibody on PFA-fixed mouse brain slices, protocol included antigene retrival and permeabilization with PBS-tween. Staining worked but only when using high concetration of antibody - 1:50-1:100
Date published: 2018-04-04
Rated 5 out of 5 by from Good signal in WBThe antibody works well in WB of mouse Striatum (25 ug), dilution 1/200 and 1/500 1% non-fat milk in TBST. Blocking 1 HS RT 5% non-fat milk in TBST. Revealed with ECL. You can see a clear band at the right size
Date published: 2017-08-08
Rated 5 out of 5 by from Good antibodyDAT Antibody (6-5G10) produced specific and positive results in multiple samples by WB and IHCP
Date published: 2017-01-24
Rated 4 out of 5 by from Works well with our human cell linesWorks well with our human cell lines.
Date published: 2015-10-23
Rated 5 out of 5 by from Great WB antibodyGreat WB antibody! Published in mouse striatum. -SCBT Publication Review
Date published: 2015-06-28
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_32258, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT
DAT/Dopamine Transporter/SLC6A3 抗体 (6-5G10) is rated 4.2 out of 5 by 13.
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_32258, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 114ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。