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CYP27A1 抗体 (D-12): sc-393222

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说明书
  • CYP27A1抗体(D-12)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列335-451位于human物种的CYP27A1的内部区域内
  • 推荐用于 human 来源的CYP27A1 WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是CYP27A1 Antibody (D-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CYP27A1 Antibody (D-12) 打包提供(请参阅下面的订购信息)。

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関連項目

P450酶是一类单加氧酶,参与多种内源性和外源性化合物的代谢。根据序列相似性,P450酶可分为不同的亚家族,包括CYP1A和CYP2A。P450家族的其他成员包括CYP19,也被称为芳香化酶(P450arom),它催化C19类固醇在各种组织(包括胎盘、性腺、脂肪组织、皮肤和大脑)中转化为雌激素。CYP19的表达受不同组织中激素调节的启动子的控制,芳香化酶活性的增加与家族性男性乳房发育症有关。此外,大多数乳腺癌患者中存在CYP19的多态性等位基因(重复(TTTA)12)。P450胆固醇7α-羟化酶,即CYP7A1,是肝脏中胆汁酸合成的限速酶,其表达由胆汁酸受体FXR介导。CYP27A1催化维生素D3的25-羟基化,并定位于肾脏和肝脏的线粒体中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CYP27A1 抗体 (D-12) 参考文献:

  1. 脑腱黄瘤病的临床和分子诊断,回顾了CYP27A1基因的突变。  |  Gallus, GN., et al. 2006. Neurol Sci. 27: 143-9. PMID: 16816916
  2. 人类CYP27A1催化β-谷甾醇和麦角甾醇的羟基化。  |  Ehrhardt, M., et al. 2016. Biol Chem. 397: 513-8. PMID: 26891232
  3. 肠道细菌产生的cyp27a1可防止结肠癌细胞凋亡。  |  Ji, YC., et al. 2016. Am J Transl Res. 8: 4434-4439. PMID: 27830027
  4. CYP27A1作用于维生素D3前体光产物,即鲁米斯特醇,产生生物活性羟基代谢产物。  |  Tuckey, RC., et al. 2018. J Steroid Biochem Mol Biol. 181: 1-10. PMID: 29452159
  5. CYP27A1 通过调节胆固醇平衡抑制膀胱癌细胞增殖。  |  Liang, Z., et al. 2019. Cell Cycle. 18: 34-45. PMID: 30563407
  6. 宿主CYP27A1表达对卵巢癌的进展至关重要。  |  He, S., et al. 2019. Endocr Relat Cancer. 26: 659-675. PMID: 31048561
  7. CYP27A1通过激活LXRs/ABCA1抑制透明细胞肾细胞癌的增殖和迁移。  |  Liang, Z., et al. 2022. Exp Cell Res. 419: 113279. PMID: 35810773
  8. 胆固醇代谢产生的27-HC通过CYP27A1抑制肾细胞癌。  |  Zhang, X., et al. 2022. FASEB J. 36: e22499. PMID: 35969149
  9. TUBB2B通过靶向HNF4A/CYP27A1调节胆固醇代谢,从而促进肝癌的发展。  |  Wang, X., et al. 2023. Cell Death Dis. 14: 179. PMID: 36872411
  10. 转录组分析确定CYP27A1是肺腺癌预后和免疫力的诊断标志物。  |  Yin, Y., et al. 2023. BMC Immunol. 24: 37. PMID: 37817081

订购信息

产品名称产品编号规格价格数量收藏夹

CYP27A1 抗体 (D-12)

sc-393222
200 µg/ml
RMB2377.00

CYP27A1 (D-12): m-IgG Fc BP-HRP 套装

sc-538170
200 µg Ab; 10 µg BP
RMB2662.00

CYP27A1 (D-12): m-IgGκ BP-HRP 套装

sc-535730
200 µg Ab; 40 µg BP
RMB2662.00

CYP27A1 (D-12): m-IgG1 BP-HRP 套装

sc-545558
200 µg Ab; 20 µg BP
RMB2662.00

What is the recommended starting dilution for Western blot for CYP27A1 (D-12): sc-393222 monoclonal antibody?

Asked by: Germaine
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-393222.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by from Decent bands of the appropriate molecularDecent bands of the appropriate molecular weight observed via Western Blot using Hep G2 and Caco-2 whole cell lysates. -SCBT QC
Date published: 2014-02-16
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CYP27A1 抗体 (D-12) is rated 3.0 out of 5 by 1.
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