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CYP27A1 Antibody (G-2) - Western Blotting - Image 291866
CYP27A1 抗体 (G-2): sc-390974. HUV-EC-C (A), Hep G2 (B) 和 Caco-2 (C) 全细胞裂解液及人肝组织提取物 (D) 中 CYP27A1 表达的 Western 印迹分析.
CYP27A1 抗体 (G-2): sc-390974. HUV-EC-C (A), Hep G2 (B) 和 Caco-2 (C) 全细胞裂解液及人肝组织提取物 (D) 中 CYP27A1 表达的 Western 印迹分析.

CYP27A1 抗体 (G-2): sc-390974

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说明书
  • CYP27A1 抗体 G-2 是小鼠单克隆 IgG2a κ，CYP27A1抗体, 在5篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列335-451位于human物种的CYP27A1的内部区域内
  • CYP27A1 抗体 (G-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 CYP27A1
  • 抗CYP27A1抗体（G-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CYP27A1 (G-2): sc-390974的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是CYP27A1 Antibody (G-2) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与CYP27A1 Antibody (G-2) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外CYP抗体，包括CYP11B, CYP17A1, CYP19, CYP1A1, CYP1A2, CYP1B1, CYP24, CYP26A1, CYP27A1, CYP27B1, CYP2A6, CYP2B1, CYP2C6, CYP2D6, CYP2F1和CYP2F2

CYP27A1抗体（G-2）是一种IgG2a κ小鼠单克隆CYP27A1抗体（也称为CYP27A1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的CYP27A1蛋白。CYP27A1抗体（G-2）既可以非偶联的抗CYP27A1抗体形式提供，也可以多种偶联形式的抗CYP27A1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。P450酶是一类单加氧酶，参与多种内源性和外源性化合物的代谢。根据序列相似性，P450酶可分为不同的亚家族，包括CYP1A和CYP2A。P450家族的其他成员包括CYP19，也被称为芳香化酶（P450arom），它催化C19类固醇在各种组织（包括胎盘、性腺、脂肪组织、皮肤和大脑）中转化为雌激素。CYP19的表达受不同组织中激素调节的启动子的控制，芳香化酶活性的增加与家族性男性乳房发育症有关。此外，大多数乳腺癌患者中存在CYP19的多态性等位基因（重复（TTTA）12）。P450胆固醇7α-羟化酶，即CYP7A1，是肝脏中胆汁酸合成的限速酶，其表达由胆汁酸受体FXR介导。CYP27A1催化维生素D3的25-羟基化，并定位于肾脏和肝脏的线粒体中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CYP27A1 抗体 (G-2) 参考文献:

  1. CYP19（芳香化酶）的基因变异与乳腺癌风险。  |  Kristensen, VN., et al. 2000. Oncogene. 19: 1329-33. PMID: 10713674
  2. 芳香化酶与妇科肿瘤  |  Braunstein, GD. 1999. Endocr Relat Cancer. 6: 315-24. PMID: 10731125
  3. 人类 CYP27A1 对维生素 D(3) 的代谢。  |  Sawada, N., et al. 2000. Biochem Biophys Res Commun. 273: 977-84. PMID: 10891358
  4. RXR 异二聚体对胆固醇吸收和 ABC1 介导的胆固醇外流的调节。  |  Repa, JJ., et al. 2000. Science. 289: 1524-9. PMID: 10968783
  5. CYP27A1缺失导致前列腺癌中胆固醇平衡失调。  |  Alfaqih, MA., et al. 2017. Cancer Res. 77: 1662-1673. PMID: 28130224
  6. 柠檬素天然产物依赖CYP27A1的抗黑色素瘤活性靶向线粒体代谢。  |  Cho, H., et al. 2021. Cell Chem Biol. 28: 1407-1419.e6. PMID: 33794192
  7. 缺乏 Cyp7a1 和 Cyp27a1 的雌性小鼠的胆汁酸稳态。  |  Rizzolo, D., et al. 2021. Acta Pharm Sin B. 11: 3847-3856. PMID: 35024311
  8. CYP27A1缺乏促进破骨细胞分化。  |  Fang, Z., et al. 2023. PeerJ. 11: e15041. PMID: 36890868
  9. CYP27A1对雄酮的27-羟化作用使其活性从促肿瘤转变为抗肿瘤。  |  Ayadi, S., et al. 2023. J Lipid Res. 64: 100479. PMID: 37981011
  10. P450 超家族：新序列, 基因图谱, 登录号和命名法的更新。  |  Nelson, DR., et al. 1996. Pharmacogenetics. 6: 1-42. PMID: 8845856
  11. P450BM-3；两个领域的故事--还是三个领域的故事？  |  Peterson, JA., et al. 1997. Steroids. 62: 117-23. PMID: 9029725
  12. 与芳香化酶表达过高有关的内分泌失调。  |  Bulun, SE., et al. 1997. J Steroid Biochem Mol Biol. 61: 133-9. PMID: 9365182

订购信息

产品名称产品编号规格价格数量收藏夹

CYP27A1 抗体 (G-2)

sc-390974
200 µg/ml
RMB2377.00

CYP27A1 (G-2): m-IgGκ BP-HRP 套装

sc-523758
200 µg Ab, 40 µg BP
RMB2662.00

CYP27A1 抗体 (G-2) AC

sc-390974 AC
500 µg/ml, 25% agarose
RMB3129.00

CYP27A1 抗体 (G-2) HRP

sc-390974 HRP
200 µg/ml
RMB2377.00

CYP27A1 抗体 (G-2) FITC

sc-390974 FITC
200 µg/ml
RMB2482.00

CYP27A1 抗体 (G-2) PE

sc-390974 PE
200 µg/ml
RMB2580.00

CYP27A1 抗体 (G-2) Alexa Fluor® 488

sc-390974 AF488
200 µg/ml
RMB2685.00

CYP27A1 抗体 (G-2) Alexa Fluor® 546

sc-390974 AF546
200 µg/ml
RMB2685.00

CYP27A1 抗体 (G-2) Alexa Fluor® 594

sc-390974 AF594
200 µg/ml
RMB2685.00

CYP27A1 抗体 (G-2) Alexa Fluor® 647

sc-390974 AF647
200 µg/ml
RMB2685.00

CYP27A1 抗体 (G-2) Alexa Fluor® 680

sc-390974 AF680
200 µg/ml
RMB2685.00

CYP27A1 抗体 (G-2) Alexa Fluor® 790

sc-390974 AF790
200 µg/ml
RMB2685.00

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-390974: CYP27A1 (G-2) mouse monoclonal antibody will work?

Asked by: TinTin
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Excellent results with Huh7 cell lysatesTested on Western blot on whole cell lysate (from Huh7 cell line) with excellent results (excellent signal, very good specificity and no background). Application: Western blot Sample: whole lysate of human hepatocytes from Huh7 cell line Gel running conditions: reduced, denaturing, 10% SDS-PAGE Loading amount: 30 µg protein/lane Transfer: semi-dry, on nitrocellulose membrane (0.45 µm) Blocking agent: 5% milk in TBST buffer, for 1h, RT Primary antibody dilution- 1: 500 in 5% milk, for 16 h at 4°C Secondary antibody: non- Santa Cruz antibody anti-mouse-HRP, 1:10000 dilution in 5% milk, for 1h, RT Detection: ECL substrate (femto sensitivity) Exposure time: 0.5 sec Bands: specific, at approx.55 kDa; non-specific bands are absent.
Date published: 2023-08-04
Rated 5 out of 5 by from Cyp27a1 antibody gives good bands on Liver WesternReceived a Cyp27a1 sample antibody with a Cyp8b1 sample antibody for the revision of one of my manuscripts. And both worked really well with mouse liver samples on westerns. I loaded 20-50mcg protein, used 1:1000 dilution of primary (Cyp27a1 and Cyp8b1 respectively) and then secondary antibody each, and developed with a "femto" enhancer instead of a "pico" (=regular enhancer) which I commonly do, and I got nice strong bands. I know many people use lower concentrations of primary antibody (which should work as well I think); but we tend to use these concentrations in our lab. Bottom line: This Cyp27a1 antibody works well, and I am happy that it did in my case, as I had several other experiments to complete for my revision.
Date published: 2017-09-21
Rated 1 out of 5 by from Non adatto ai campioni/metodiche utilizzatiBuongiorno, ho testato CYP27A1 e CYP46A1 su cervelli umani fissati in PFA 4% ed inclusi in paraffina, sia in IIC che IF, ma non ho avuto risultati soddisfacenti. Cordiali saluti, Alessandra Milesi
Date published: 2017-08-28
Rated 4 out of 5 by from Strong CYP27A1 WB signals made in our labStrong CYP27A1 WB signals have been made with human sample in our lab
Date published: 2017-02-16
Rated 5 out of 5 by from strong band at 55kDThere was a strong band at 55kD in human samples by WB
Date published: 2016-12-14
Rated 5 out of 5 by from Robust signal obtained via Western Blot using HUVRobust signal obtained via Western Blot using HUV-EC-C, HepG2 and CaCo-2 whole cell lysates and human liver tissue extract. -SCBT QC
Date published: 2014-03-03
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CYP27A1 抗体 (G-2) is rated 4.2 out of 5 by 6.
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