Date published: 2025-9-7
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Chk1 Antibody (DCS-310) - Western Blotting - Image 353140
Chk1 抗体 (DCS-310): sc-56291. K-562 (A), HeLa (B) 和 MDA-MB-231 (C) 全细胞裂解液中 Chk1 表达的 Western 印迹分析.
Chk1 抗体 (DCS-310): sc-56291. K-562 (A), HeLa (B) 和 MDA-MB-231 (C) 全细胞裂解液中 Chk1 表达的 Western 印迹分析.

Chk1 抗体 (DCS-310): sc-56291

4.1(7)
寫評論提問

说明书
  • Chk1抗体(DCS-310)是小鼠单克隆IgG2b κ, 在43篇文献中引用，规格为200 µg/ml
  • 针对全长Chk1，human起源
  • 抗-Chk1 抗体 (DCS-310) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Chk1
  • 抗Chk1抗体（DCS-310）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Chk1 (DCS-310): sc-56291的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 Chk1 抗体 (DCS-310) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Chk1 抗体 (DCS-310) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Chk 抗体 包括 Chk2

Chk1 抗体（DCS-310）是一种小鼠单克隆 IgG2b kappa 轻链抗体，可通过各种应用检测小鼠、大鼠和人样本中的 Chk1，包括免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附试验（ELISA）。抗 Chk1 抗体 (DCS-310) 有非共轭型和多种共轭型，如琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物。Chk1 在细胞对 DNA 损伤的反应中起着关键作用，是 G2 DNA 损伤检查点的关键调节因子。通过磷酸化 Cdc25C，Chk1 可抑制其活性，进而阻止依赖细胞周期蛋白的激酶的激活，阻碍有丝分裂的进行。这种调控机制对维持基因组稳定性至关重要，因为细胞可以在进入有丝分裂之前修复 DNA 损伤，从而防止受损 DNA 传播到子细胞。研究表明，Chk1 可与包括 Wee1 在内的其他参与细胞周期调控的蛋白相互作用，这进一步凸显了 Chk1 在错综复杂的细胞周期调控网络中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Chk1 抗体 (DCS-310)

sc-56291
200 µg/ml
$316.00

Chk1 (DCS-310): m-IgG Fc BP-HRP 套装

sc-525652
200 µg Ab; 10 µg BP
$354.00

Chk1 (DCS-310): m-IgG2b BP-HRP 套装

sc-549607
200 µg Ab; 10 µg BP
$354.00

Chk1 抗体 (DCS-310) AC

sc-56291 AC
500 µg/ml, 25% agarose
$416.00

Chk1 抗体 (DCS-310) HRP

sc-56291 HRP
200 µg/ml
$316.00

Chk1 抗体 (DCS-310) FITC

sc-56291 FITC
200 µg/ml
$330.00

Chk1 抗体 (DCS-310) PE

sc-56291 PE
200 µg/ml
$343.00

Chk1 抗体 (DCS-310) Alexa Fluor® 488

sc-56291 AF488
200 µg/ml
$357.00

Chk1 抗体 (DCS-310) Alexa Fluor® 546

sc-56291 AF546
200 µg/ml
$357.00

Chk1 抗体 (DCS-310) Alexa Fluor® 594

sc-56291 AF594
200 µg/ml
$357.00

Chk1 抗体 (DCS-310) Alexa Fluor® 647

sc-56291 AF647
200 µg/ml
$357.00

Chk1 抗体 (DCS-310) Alexa Fluor® 680

sc-56291 AF680
200 µg/ml
$357.00

Chk1 抗体 (DCS-310) Alexa Fluor® 790

sc-56291 AF790
200 µg/ml
$357.00

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 7
Date published: 2017-02-09
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Rated 5 out of 5 by from Great WB choice!Works great for your WB detection. It will provide strong clear bands in mouse, rat, or human samples. It has been published with over 10 times.
Date published: 2017-01-20
Rated 5 out of 5 by from Published data for use in WB in human MRCPublished data for use in WB in human MRC-5 fibroblasts.
Date published: 2015-07-06
Rated 4 out of 5 by from Good specificity in WB in human U2OSGood specificity in WB in human U2OS, 293T, HeLa and T98G cells. -SCBT Publication Review
Date published: 2015-05-13
Rated 4 out of 5 by from Good publishable data in WB and IF in humanGood publishable data in WB and IF in human lung and colon. -SCBT Publication Review
Date published: 2015-03-24
Rated 4 out of 5 by from Strong band in WB in mouse transfected 293T and KStrong band in WB in mouse transfected 293T and K-562 whole cell lysates. -SCBT QC
Date published: 2015-01-29
Rated 4 out of 5 by from Worked well in WB in mouse KH2 ES cellsWorked well in WB in mouse KH2 ES cells. -SCBT Publication Review
Date published: 2015-01-22
Rated 3 out of 5 by from Positive signal in WB in HeLaPositive signal in WB in HeLa, K-562 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2013-02-18
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Chk1 抗体 (DCS-310) is rated 4.1 out of 5 by 7.
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