Date published: 2025-9-7
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Chk1 Antibody (G-4) - Western Blotting - Image 392307
Chk1 抗体 (G-4) Alexa Fluor® 488: sc-8408 AF488. K-562 (A), HCT-116 (B) 和 MOLT-4 (C) 全细胞裂解液中 Chk1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Chk1 抗体 (G-4) Alexa Fluor® 488: sc-8408 AF488. K-562 (A), HCT-116 (B) 和 MOLT-4 (C) 全细胞裂解液中 Chk1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Chk1 抗体 (G-4): sc-8408

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说明书
  • Chk1 抗体 G-4 是小鼠单克隆 IgG2a κ，Chk1抗体, 在841篇文献中引用，规格为200 µg/ml
  • 免疫1-476氨基酸，对应于human来源Chk1全长
  • Chk1 抗体 (G-4) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 Chk1
  • Chk1抗体（G-4）可与琼脂糖结合用于IP，与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Chk1 (G-4): sc-8408的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Chk1 抗体 (G-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Chk1 抗体 (G-4) 组合成套装提供。 (请参阅下面的订购信息)。

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基因信息
说明书与实验方案
研究信息

関連項目

此外 Chk 抗体 包括 Chk2

Chk1抗体（G-4）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类样本中的Chk1，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）。Chk1 (G-4) 抗体有非结合型和多种结合型，如琼脂糖、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Chk1在细胞周期的调节中发挥着关键作用，特别是在G2 DNA损伤检查点，Chk1作为细胞周期依赖性激酶（Cdks）的关键调节因子。通过磷酸化Cdc25C来应对DNA损伤，Chk1抑制Cdc25C的活性，有效阻止向有丝分裂的过渡，从而进行DNA修复。这一功能对于维持基因组稳定性和防止受损DNA的传播至关重要，否则会导致肿瘤形成。此外，Chk1已被证明能够磷酸化Wee1，进一步说明了Chk1在细胞周期调节中的作用。Chk1能够调节这些关键过程，这凸显了Chk1在细胞对DNA损伤的反应中的重要性，并凸显了Chk1作为癌症治疗干预靶标的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Chk1 抗体 (G-4) 参考文献:

  1. 双突变胸腺细胞中 Chk1 和 Chk2 之间的交叉对话  |  Zaugg, K., et al. 2007. Proc Natl Acad Sci U S A. 104: 3805-10. PMID: 17360434
  2. 克制：Chk1 在调节脊椎动物细胞无扰动有丝分裂的开始和进展中的作用。  |  Zachos, G. and Gillespie, DA. 2007. Cell Cycle. 6: 810-3. PMID: 17377502
  3. HCLK2 对哺乳动物的 S 期检查点至关重要，并影响 Chk1 的稳定性。  |  Collis, SJ., et al. 2007. Nat Cell Biol. 9: 391-401. PMID: 17384638
  4. cdc25 是一种特异性酪氨酸磷酸酶，可直接激活 p34cdc2。  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
  5. DNA损伤信号传导和癌症中的ATM-Chk2和ATR-Chk1通路。  |  Smith, J., et al. 2010. Adv Cancer Res. 108: 73-112. PMID: 21034966
  6. ATR/CHK1抑制剂与癌症治疗。  |  Qiu, Z., et al. 2018. Radiother Oncol. 126: 450-464. PMID: 29054375
  7. UBE2T调控的H2AX单泛素化通过促进CHK1活化诱导肝癌放射抗性。  |  Sun, J., et al. 2020. J Exp Clin Cancer Res. 39: 222. PMID: 33087136
  8. 细胞周期的新转折  |  Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
  9. Chk1 是 G2 DNA 损伤检查点中的一种 wee1 激酶，通过 Y15 磷酸化抑制 cdc2。  |  O'Connell, MJ., et al. 1997. EMBO J. 16: 545-54. PMID: 9034337
  10. 哺乳动物中 Chk1 检查点途径的保存：DNA 损伤与通过 Cdc25 调节 Cdk 的联系。  |  Sanchez, Y., et al. 1997. Science. 277: 1497-501. PMID: 9278511
  11. 有丝分裂和 G2 检查点控制：通过 Cdc25C 丝氨酸-216 上的磷酸化调节 14-3-3 蛋白的结合。  |  Peng, CY., et al. 1997. Science. 277: 1501-5. PMID: 9278512
  12. ATM与Chk2蛋白激酶对细胞周期调控的联系  |  Matsuoka, S., et al. 1998. Science. 282: 1893-7. PMID: 9836640

订购信息

产品名称产品编号规格价格数量收藏夹

Chk1 抗体 (G-4)

sc-8408
200 µg/ml
RMB2377.00

Chk1 (G-4): m-IgG Fc BP-HRP 套装

sc-525453
200 µg Ab; 10 µg BP
RMB2662.00

Chk1 (G-4): m-IgG2a BP-HRP 套装

sc-546265
200 µg Ab; 10 µg BP
RMB2662.00

Chk1 抗体 (G-4) AC

sc-8408 AC
500 µg/ml, 25% agarose
RMB3129.00

Chk1 抗体 (G-4) FITC

sc-8408 FITC
200 µg/ml
RMB2482.00

Chk1 抗体 (G-4) PE

sc-8408 PE
200 µg/ml
RMB2580.00

Chk1 抗体 (G-4) Alexa Fluor® 488

sc-8408 AF488
200 µg/ml
RMB2685.00

Chk1 抗体 (G-4) Alexa Fluor® 546

sc-8408 AF546
200 µg/ml
RMB2685.00

Chk1 抗体 (G-4) Alexa Fluor® 594

sc-8408 AF594
200 µg/ml
RMB2685.00

Chk1 抗体 (G-4) Alexa Fluor® 647

sc-8408 AF647
200 µg/ml
RMB2685.00

Chk1 抗体 (G-4) Alexa Fluor® 680

sc-8408 AF680
200 µg/ml
RMB2685.00

Chk1 抗体 (G-4) Alexa Fluor® 790

sc-8408 AF790
200 µg/ml
RMB2685.00

Has this antibody been tested for the sole kinase domain as well (approx. residues 1-270)?

Asked by: maximizor
Thank you for your question. The antibody was raised against amino acids 1-476 representing full length Chk1 of human origin.
Answered by: BlakeJ
Date published: 2023-05-01

Does this antibody recognize hamster total Chk1?

Asked by: DrAKA
Thank you for your question. We do not have any data or product citations using this antibody, Chk1 (G-4): sc-8408, for detection of Chk1 in hamster. The antibody is raised against full-length Chk1 of human origin, and based on a predicted protein sequence for Chk1 in hamster (accession number: XP_003511081) this sequence shares 95% sequence identity with the hamster protein. That is a significant, and if the predicted sequence is correct, then there is a very good chance the antibody may also cross-react with hamster.
Answered by: Technical Support
Date published: 2017-09-07

What do you recommend for a positive control for Western blot?

Asked by: Dr Ninau Qelp
We have several recommended positive controls that can be used with Chk1 Antibody (G-4): sc-8408 for Western blot (WB). MDA-MB-231 Cell Lysate: sc-2232 HCT-116 Whole Cell Lysate: sc-364175 PANC-1 Whole Cell Lysate: sc-364380 HeLa Whole Cell Lysate: sc-2200 NIH/3T3 Whole Cell Lysate: sc-2210
Answered by: Tech Service 2
Date published: 2016-12-16

Can Chk1 (G-4) be used for chicken DT40 cell line?

Asked by: Dr Ninau Qelp
Yes! We have several publications referencing Chk1 Antibody (G-4): sc-8408 being used with chicken DT40 samples. Please see PMID: 26039276, 21765472, 17575048, 16619043 and 12554671.
Answered by: Tech Service 2
Date published: 2016-12-16
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Rated 3 out of 5 by from EO771 Chk1 WesternI was able to detect bands using 1:750 dilution (in 5% BSA in TBST) and 1:20,000 anti-mouse-HRP secondary from mouse cell lysates (EO771) with 13 minute exposure using SuperSignal™ West Dura Extended Duration Substrate.
Date published: 2025-07-30
Rated 5 out of 5 by from Excellent antibodyThe antibody was excellent in Western blot using whole cell lysates from human Caco-2 cell lines.
Date published: 2022-12-25
Rated 4 out of 5 by from Perfect on WBWe used it at 1:100 diluition and it works very well. We are satisfied.
Date published: 2020-10-13
Rated 5 out of 5 by from excellent antibodythis antibody worked nicely with human cell lysates!
Date published: 2020-09-16
Rated 5 out of 5 by from Great feature for Western blotExcellent in Western blot for human and mouse cell lysates!
Date published: 2020-09-07
Rated 5 out of 5 by from good antibodyGood antibody with clear background, one single band in pancreatic cancer cells (Panc1). Used 1:200 in WB
Date published: 2020-02-10
Rated 4 out of 5 by from Chk1good antibody for Western blot, very clean. Also works for human cells.
Date published: 2020-01-22
Rated 5 out of 5 by from excellentOnly single band. Very good antibody. .
Date published: 2019-12-03
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Chk1 抗体 (G-4) is rated 4.7 out of 5 by 40.
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