Date published: 2026-10-24

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Chk1 Antibody (2G11D5) - Western Blotting - Image 353147
Chk1 抗体 (2G11D5): sc-56288. HCT-116 (A) 和 PANC-1 (B) 全细胞裂解液中 Chk1 表达的 Western 印迹分析.
Chk1 抗体 (2G11D5): sc-56288. HCT-116 (A) 和 PANC-1 (B) 全细胞裂解液中 Chk1 表达的 Western 印迹分析.

Chk1 抗体 (2G11D5): sc-56288

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说明书
  • Chk1抗体(2G11D5)是小鼠单克隆IgG1 κ, 在10篇文献中引用，规格为200 µg/ml
  • 针对human来源Ni-NTA纯化重组Chk1的
  • 推荐用于 mouse, rat 和 human 来源的Chk1 WB 和 IP检测
  • 看 Chk1 (G-4): sc-8408，可查看有标记的 Chk1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP是Chk1 Antibody (2G11D5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Chk1 Antibody (2G11D5) 搭配使用（请参阅下面的订购信息）。
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関連項目

额外Chk抗体，包括Chk2

细胞周期事件受到细胞周期蛋白依赖性激酶（Cdks）的顺序激活和失活以及细胞周期蛋白的水解调节，而Chk1和Chk2作为Cdks的调节因子参与这些过程。特别地，Chk1和Chk2在G2 DNA损伤检查点中发挥着至关重要的作用，它们通过响应DNA损伤来磷酸化Cdc25C。这种磷酸化作用抑制了Cdc25C的活性，从而阻止了细胞进入有丝分裂阶段。此外，Cdc25A、Cdc25B和Cdc25C蛋白酪氨酸磷酸酶则通过去磷酸化调节性酪氨酸残基上的Cdc2 p34来发挥有丝分裂激活剂的作用。值得一提的是，研究还表明Chk1可以在体外磷酸化Wee1，这为进一步证实细胞内由于Chk1过度表达而导致的Wee1超磷酸化状态提供了证据，这种状态是由Chk1对Wee1的磷酸化作用所引起的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Chk1 抗体 (2G11D5) 参考文献:

  1. Chk 1和Chk 2在缺氧和再氧化的作用。  |  Hammond, EM., et al. 2006. Cancer Lett. 238: 161-7. PMID: 16085356
  2. 在癌症治疗中释放Chk1。  |  Carrassa, L. and Damia, G. 2011. Cell Cycle. 10: 2121-8. PMID: 21610326
  3. 通过磷酸化调节Chk1的新见解。  |  Goto, H., et al. 2015. Cell Struct Funct. 40: 43-50. PMID: 25748360
  4. 癌症治疗中ATR-CHK1轴的靶向作用。  |  Rundle, S., et al. 2017. Cancers (Basel). 9: PMID: 28448462
  5. 研究中的CHK-1抑制剂在治疗实体肿瘤方面的治疗潜力。  |  Babiker, HM., et al. 2017. Expert Opin Investig Drugs. 26: 1063-1072. PMID: 28745064
  6. TRIM21通过优先靶向CLASPIN进行K63连接的泛素化来抑制CHK1的活化。  |  Zhu, X., et al. 2022. Nucleic Acids Res. 50: 1517-1530. PMID: 35048968
  7. 组蛋白H3丝氨酸-57是CHK1的底物，其磷酸化会影响DNA修复。  |  Parisis, N., et al. 2023. Nat Commun. 14: 5104. PMID: 37607906
  8. BRD7 通过抑制 USP1 介导的 CHK1 泛素化来抑制肿瘤对 CHK1 抑制剂的化疗敏感性。  |  Li, L., et al. 2023. Cell Death Discov. 9: 313. PMID: 37626049
  9. 通过ATR/CHK1通路调节肺癌治疗的新视野。  |  Thapa, R., et al. 2023. Future Med Chem. 15: 1807-1818. PMID: 37877252
  10. 联合抑制Wee1和Chk1作为多发性骨髓瘤的治疗策略。  |  Bruyer, A., et al. 2023. Front Oncol. 13: 1271847. PMID: 38125947

订购信息

产品名称产品编号规格价格数量收藏夹

Chk1 抗体 (2G11D5)

sc-56288
200 µg/ml
CNY2422.00

Chk1 (2G11D5): m-IgG Fc BP-HRP 套装

sc-551939
200 µg Ab; 10 µg BP
CNY2715.00

Are there any issues with using sc-56288: Chk1 (2G11D5) mouse monoclonal primary antibody on mouse samples?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-56288 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by FL from Robust signal in WB in NIH/3T3 and HeLa wholeRobust signal in WB in NIH/3T3 and HeLa whole cell lysates under reducing and non-reducing conditions. -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by LR from Strong band in WB in HeLaStrong band in WB in HeLa, K-562 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-02-18
Rated 5 out of 5 by QZ from Positive signal in WB in 293T mouse transfectedPositive signal in WB in 293T mouse transfected and K-562 whole cell lysates. -SCBT QC
Date published: 2015-02-05
Rated 5 out of 5 by HF from Good publishable data in WB in human lymphoblastoidGood publishable data in WB in human lymphoblastoid cells, fibroblasts. -SCBT Publication Review
Date published: 2013-04-03
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Chk1 抗体 (2G11D5) is rated 5.0 out of 5 by 4.
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