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CD73 Antibody (IE9) - Western Blotting - Image 411284
CD73抗体（IE9）：sc-32299。未经处理（A）和化学处理（B、C、D）的HEK293T全细胞裂解液中CD73表达的Western印迹分析。所用检测试剂：m-IgGκ BP-HRP：sc-516102。β-Actin（C4）：sc-47778用作加载对照。所用检测试剂：m-IgG Fc BP-HRP：sc-525409。
CD73抗体（IE9）：sc-32299。未经处理（A）和化学处理（B、C、D）的HEK293T全细胞裂解液中CD73表达的Western印迹分析。所用检测试剂：m-IgGκ BP-HRP：sc-516102。β-Actin（C4）：sc-47778用作加载对照。所用检测试剂：m-IgG Fc BP-HRP：sc-525409。

CD73 抗体 (IE9): sc-32299

4.9(8)
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说明书
  • CD73抗体(IE9)是小鼠单克隆IgG3 κ, 在35篇文献中引用，规格为200 µg/ml
  • 针对human CD73从胎盘中纯化
  • 推荐用于 human 来源的CD73 WB, IP, IF 和 IHC(P)检测; 不会与 mouse CD73产生交联反应
  • 看 CD73 (D-12): sc-398260，可查看有标记的 CD73 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取CD73 (IE9): sc-32299的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD73 抗体 (IE9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD73抗体（IE9）是一种IgG3 κ小鼠单克隆CD73抗体（也称为CD73抗体或5'-核苷酸酶抗体），可通过WB、IP、IF和IHC（P）检测人类来源的CD73。CD73抗体（IE9）可以作为非偶联的抗CD73抗体形式使用。CD73（也称为5'-核苷酸酶、E5NT、NT、NT5、NTE、eN和eNT）是一种糖脂磷脂酰肌醇（GPI）抗体，可催化外源性嘌呤和嘧啶核苷酸的去磷酸化，以获得相应的生物活性核苷酸。CD73是两个相同亚基的二聚体，依赖GPI与细胞膜的外侧连接。与其他GPI抗体蛋白一样，CD73介导T细胞激活的共刺激信号。CD73具有少量结构变体，但通过内皮细胞（EC）、B和T细胞的亚群、胚珠中心毛细突状细胞和胸腺髓质网状纤维细胞的差异调控，发挥不同的生物学功能。例如，IgG介导的CD73中和干扰淋巴细胞与EC的粘附，并阻断胚珠中心B细胞和毛细突状细胞的聚集。此外，IgG介导的淋巴细胞CD73靶向（而非内皮细胞CD73靶向）会导致CD73的脱落和蛋白酪氨酸磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD73 抗体 (IE9) 参考文献:

  1. TNF-α 对人内皮细胞外-5'-核苷酸酶的调控  |  Kalsi, K., et al. 2002. Mol Cell Biochem. 232: 113-9. PMID: 12030367
  2. 粘附的白细胞通过外-5'-核苷酸酶/CD73 依赖性机制阻止腺苷形成并损害内皮屏障功能。  |  Henttinen, T., et al. 2003. J Biol Chem. 278: 24888-95. PMID: 12707258
  3. IFN-α 诱导内皮产生腺苷：CD73（外向-5'-核苷酸酶）上调介导的机制。  |  Niemelä, J., et al. 2004. J Immunol. 172: 1646-53. PMID: 14734746
  4. 在博莱霉素诱导的肺损伤模型中，外-5'-核苷酸酶（CD73）介导的腺苷生成对组织具有保护作用。  |  Volmer, JB., et al. 2006. J Immunol. 176: 4449-58. PMID: 16547283
  5. 甲状腺乳头状癌中 CD73 的表达及其外向-5'-核苷酸酶活性升高。  |  Kondo, T., et al. 2006. Histopathology. 48: 612-4. PMID: 16623792
  6. CD73/ecto-5'-nucleotidase 可防止血管炎症和新生血管形成。  |  Zernecke, A., et al. 2006. Circulation. 113: 2120-7. PMID: 16636171
  7. 滤泡树突状细胞与骨髓基质细胞祖细胞和肌成纤维细胞有关。  |  Muñoz-Fernández, R., et al. 2006. J Immunol. 177: 280-9. PMID: 16785523
  8. 胰腺分泌的消耗 ATP 和产生 ATP 的酶。  |  Yegutkin, GG., et al. 2006. J Biol Chem. 281: 29441-7. PMID: 16885159
  9. CD73抑制cGAS-STING，并与CD39协同促进胰腺癌。  |  Jacoberger-Foissac, C., et al. 2023. Cancer Immunol Res. 11: 56-71. PMID: 36409930
  10. TRIM21对CD73的蛋白水解调节可协调肿瘤免疫原性。  |  Fu, Z., et al. 2023. Sci Adv. 9: eadd6626. PMID: 36608132
  11. CD39/CD73/A2AR通路与癌症免疫疗法。  |  Xia, C., et al. 2023. Mol Cancer. 22: 44. PMID: 36859386
  12. 小鼠淋巴细胞上的 CD73 表达和 fyn 依赖性信号传导。  |  Yamashita, Y., et al. 1998. Eur J Immunol. 28: 2981-90. PMID: 9808167

订购信息

产品名称产品编号规格价格数量收藏夹

CD73 抗体 (IE9)

sc-32299
200 µg/ml
RMB2377.00

CD73 (IE9): m-IgGκ BP-HRP 套装

sc-551791
200 µg Ab; 40 µg BP
RMB2662.00

Est-ce que Anticorps CD73 (IE9): sc-32299 peut être utilisé pour des tests ELISA ?  En quelle quantité est-t-il fourni ? 1ml ? 

Asked by: ZOED
Merci pour votre question. Anticorps CD73 (IE9): sc-32299 est recommandé pour la détection de CD73 d'origine human par WB, IP, IF et IHC(P); non cross-reactif avec mouse CD73. Nous n'avons pas de données pour soutenir l'utilisation dans ELISA. L'anticorps est fourni à 200 µg dans 1 ml.
Answered by: Tech Support Europe
Date published: 2022-12-02

I used CD73 Antibody (IE9): sc-32299 for my research. I used auto IHC machine. It is of Roche Company. As I know, the antibody will help to stain cell membrane and sometimes cytoplasm. However, my samples showed nuclear staining. Give me some advices!

Asked by: quocvuongsung
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-07

Hi, I would like to know if it is possible to have the product sc-32299 in a carrier-free version (without gelatin)? Thank you

Asked by: Anonymous
Thank you for your question. Yes, this can be provided carrier-free (without gelatin) as a special order. Please contact our Special Orders Department to inquire about availability and to receive a quote. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Technical Support
Date published: 2019-12-07

Hello: Was CD73 Antibody (IE9): sc-32299 tested on rat tissues? It was found to inhibit the catalytic activity of CD73 (in http://www.jimmunol.org/content/191/8/4165.long). This would be great if I could use it in rat tissue homogenates? thanks, Fran.

Asked by: FranVdB
Thank you for your question. We do not have any data indicating that this mouse monoclonal antibody, CD73 (IE9): sc-32299, is reactive with rat CD73. We recommend the antibody for detection of CD73 of human origin only. In the publication you have cited, the authors specifically mention that IE9 clone "failed to detect the murine CD73 variant ".
Answered by: Technical Support
Date published: 2017-06-28

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-23
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Rated 5 out of 5 by from Works well for human mesenchymal Stem cellsReceived sample for this 2 months ago. Worked pretty well with human BMMSCs at 1:50. Definitely going to purchase
Date published: 2020-03-10
Rated 4 out of 5 by from goodused dilution 1: 150 overnight for immunofluorescence
Date published: 2018-04-10
Rated 5 out of 5 by from Works well in WesternThis is an excellent antibody for use in Western Blot - good band strength and clarity with little to no background.
Date published: 2016-12-27
Rated 5 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the CD73 protein via Western Blot analysis. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Tested for western blotting with MBTested for western blotting with MB-MDA-231, MB-MDA-453, MB-MDA-468, T47D, and MCF-7 cells, and breast cancer tissue extracts. -SCBT Publication Review
Date published: 2015-04-09
Rated 5 out of 5 by from IHC: acetone fixed ovarian cancer cryosectionsIHC: acetone fixed ovarian cancer cryosections. -SCBT Publication Review
Date published: 2015-01-24
Rated 5 out of 5 by from Clean band in human recombinant CD73 viaClean band in human recombinant CD73 via western blotting. -SCBT QC
Date published: 2015-01-23
Rated 5 out of 5 by from Published IHC data with human trabecularPublished IHC data with human trabecular bone sections. -SCBT Publication Review
Date published: 2014-09-25
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CD73 抗体 (IE9) is rated 4.9 out of 5 by 8.
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