Date published: 2025-9-7

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CD5 抗体 (CD5/54/F6): sc-53204

4.9(7)
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说明书
  • CD5抗体(CD5/54/F6)是小鼠单克隆IgG1 κ, 在3篇文献中引用,规格为200 µg/ml
  • 与human的CD5的细胞内区域对应的合成肽的抗原决定簇
  • 抗-CD5 抗体 (CD5/54/F6) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat, human, rabbit, equine 和bovine 来源的 CD5
  • 抗CD5抗体(CD5/54/F6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD5 (CD5/54/F6): sc-53204免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 CD5 抗体 (CD5/54/F6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD5 抗体 (CD5/54/F6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CD5抗体(CD5/54/F6)是一种鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测鼠、大鼠、人、兔、马和牛源性CD5蛋白。抗 CD5抗体(CD5/54/F6)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。CD5,也称为Lyt-1,是一种跨膜糖蛋白,主要表达于约70%的正常外周血淋巴细胞和几乎所有胸腺和外周血中的T淋巴细胞。CD5在T细胞激活中起着至关重要的作用;当T细胞通过T细胞受体(TCR)激活时,CD5会发生酪氨酸磷酸化,这是调节T细胞反应所必需的。值得注意的是,缺乏CD5会导致T细胞对TCR介导的激活产生过度反应,这凸显了CD5在维持免疫平衡中的重要性。CD5与TCR/CD3-ζ链和Src家族激酶Lck p56相互作用,当与CD5结合时,可显著增强Lck的激酶活性10至15倍。此外,CD5还是B细胞抗原CD72的受体,尽管这种相互作用的精确结果仍有待充分阐明,但表明其在胸腺选择过程中可能发挥潜在作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD5 抗体 (CD5/54/F6)

sc-53204
200 µg/ml
RMB2377.00

CD5 (CD5/54/F6): m-IgG Fc BP-HRP 套装

sc-526778
200 µg Ab; 10 µg BP
RMB2662.00

CD5 (CD5/54/F6): m-IgG1 BP-HRP 套装

sc-532151
200 µg Ab; 20 µg BP
RMB2662.00

CD5 抗体 (CD5/54/F6) AC

sc-53204 AC
500 µg/ml, 25% agarose
RMB3129.00

CD5 抗体 (CD5/54/F6) HRP

sc-53204 HRP
200 µg/ml
RMB2377.00

CD5 抗体 (CD5/54/F6) FITC

sc-53204 FITC
200 µg/ml
RMB2482.00

CD5 抗体 (CD5/54/F6) PE

sc-53204 PE
200 µg/ml
RMB2580.00

CD5 抗体 (CD5/54/F6) Alexa Fluor® 488

sc-53204 AF488
200 µg/ml
RMB2685.00

CD5 抗体 (CD5/54/F6) Alexa Fluor® 546

sc-53204 AF546
200 µg/ml
RMB2685.00

CD5 抗体 (CD5/54/F6) Alexa Fluor® 594

sc-53204 AF594
200 µg/ml
RMB2685.00

CD5 抗体 (CD5/54/F6) Alexa Fluor® 647

sc-53204 AF647
200 µg/ml
RMB2685.00

CD5 抗体 (CD5/54/F6) Alexa Fluor® 680

sc-53204 AF680
200 µg/ml
RMB2685.00

CD5 抗体 (CD5/54/F6) Alexa Fluor® 790

sc-53204 AF790
200 µg/ml
RMB2685.00

Hi, I want to know about this CD5 Antibody (CD5/54/F6), is there any carrier-free (BSA and azide free) version of this product. thanks

Asked by: Jiadong
Thank you for your question. For custom products please contact special orders department by emailing [email protected]. They can provide a price quote and ship time.
Answered by: BlakeJ
Date published: 2024-03-28

I am using CD5 (CD5/54/F6): sc-53204 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good results on FFPE lymph node from a dogWe tested this antibody for IHC(P) on canine tissue and the signal seems specific and clear.
Date published: 2023-11-02
Rated 4 out of 5 by from excellent antibodyuse this antibody, we got an very clear band in WB experiment
Date published: 2018-01-08
Rated 5 out of 5 by from Excellent immunoperoxidase membrane and cytoplasmicExcellent immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Good specificity and signal strength in CCRFGood specificity and signal strength in CCRF-CEM whole cell lysate. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Good antibody for westernGood antibody for western, clean background and specific band in MOLT-4 whole cell lysate. - SCBT QC
Date published: 2015-04-24
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using Jurkat and ALL-SIL whole cell lysates under reducing and non-reducing conditions. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Band detected at expected mw in human CD5Band detected at expected mw in human CD5 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-02-13
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CD5 抗体 (CD5/54/F6) is rated 4.9 out of 5 by 7.
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