Date published: 2025-9-7

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CD5 抗体 (UCH-T2): sc-1180

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说明书
  • CD5 抗体 UCH-T2 是小鼠单克隆 IgG1 κ,CD5抗体, 在4篇文献中引用,规格为200 µg/ml
  • 针对human胸腺细胞/塞扎里T细胞
  • CD5 抗体 (UCH-T2) 推荐用于 WB, IP, IF, IHC(P) 和 FCM,检测mouse, rat 和human 来源的 CD5
  • 抗CD5抗体(UCH-T2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD5 (UCH-T2): sc-1180免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 CD5 抗体 (UCH-T2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD5 抗体 (UCH-T2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CD5抗体(UCH-T2)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和流式细胞术(FCM)检测小鼠、大鼠和人源CD5蛋白。CD5 (UCH-T2) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合型。CD5,也称为Lyt-1,是一种重要的跨膜糖蛋白,主要表达于约70%的正常外周血淋巴细胞以及几乎所有胸腺和外周血中的T淋巴细胞。CD5的主要功能是调节T细胞的激活;当T细胞通过T细胞受体(TCR)激活时,CD5会经历酪氨酸磷酸化,这是调节T细胞反应的关键。值得注意的是,缺少CD5会导致T细胞对TCR介导的激活反应过度,这凸显了CD5在维持免疫平衡方面的作用。CD5与TCR/CD3-ζ链和Src家族激酶Lck p56相互作用,当与CD5结合时,可显著增强Lck的激酶活性10至15倍。此外,CD5还是B细胞抗原CD72的受体,尽管这种相互作用的精确结果仍有待充分阐明,但表明其在胸腺选择过程中可能发挥潜在作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD5 抗体 (UCH-T2) 参考文献:

  1. CD5 在受到 T 细胞抗原受体复合物的刺激后会在酪氨酸上磷酸化。  |  Davies, AA., et al. 1992. Proc Natl Acad Sci U S A. 89: 6368-72. PMID: 1378627
  2. CD5+ B 细胞与免疫系统  |  Lydyard, PM., et al. 1993. Immunol Lett. 38: 159-66. PMID: 7507466
  3. T 细胞抗原 CD5 是蛋白酪氨酸激酶 p56lck 的受体和底物。  |  Raab, M., et al. 1994. Mol Cell Biol. 14: 2862-70. PMID: 7513045
  4. 抗 CD5 治疗可降低胶原 II 型诱导的 DBA/1 小鼠关节炎的既定疾病严重程度。  |  Plater-Zyberk, C., et al. 1994. Clin Exp Immunol. 98: 442-7. PMID: 7527741
  5. CD5 在 TCR 介导的信号转导和胸腺细胞选择中的作用  |  Tarakhovsky, A., et al. 1995. Science. 269: 535-7. PMID: 7542801
  6. B 细胞上的 CD5 表达可能是单克隆抗体病相关多发性神经病患者分泌抗髓鞘抗体的激活标志。  |  Ekerfelt, C., et al. 1995. Clin Exp Immunol. 101: 346-50. PMID: 7544252
  7. 类风湿性关节炎和斯约格伦综合征的 T 细胞和 B 细胞亚群中 CD5 和 CD72 的表达。  |  Jamin, C., et al. 1993. Clin Exp Immunol. 92: 245-50. PMID: 7683586
  8. 使用交叉反应抗肽抗体检测许多动物物种的 T 细胞和 B 细胞。  |  Jones, M., et al. 1993. J Immunol. 150: 5429-35. PMID: 8515069
  9. 鸟类 CD5 的结构意味着其功能是保守的。  |  Koskinen, R., et al. 1998. J Immunol. 160: 4943-50. PMID: 9590242

订购信息

产品名称产品编号规格价格数量收藏夹

CD5 抗体 (UCH-T2)

sc-1180
200 µg/ml
RMB2377.00

CD5 (UCH-T2): m-IgG Fc BP-HRP 套装

sc-528123
200 µg Ab; 10 µg BP
RMB2662.00

CD5 (UCH-T2): m-IgGκ BP-HRP 套装

sc-520426
200 µg Ab, 40 µg BP
RMB2662.00

CD5 (UCH-T2): m-IgG1 BP-HRP 套装

sc-542775
200 µg Ab; 20 µg BP
RMB2662.00

CD5 抗体 (UCH-T2) AC

sc-1180 AC
500 µg/ml, 25% agarose
RMB3129.00

CD5 抗体 (UCH-T2) HRP

sc-1180 HRP
200 µg/ml
RMB2377.00

CD5 抗体 (UCH-T2) FITC

sc-1180 FITC
200 µg/ml
RMB2482.00

CD5 抗体 (UCH-T2) PE

sc-1180 PE
200 µg/ml
RMB2580.00

CD5 抗体 (UCH-T2) Alexa Fluor® 488

sc-1180 AF488
200 µg/ml
RMB2685.00

CD5 抗体 (UCH-T2) Alexa Fluor® 546

sc-1180 AF546
200 µg/ml
RMB2685.00

CD5 抗体 (UCH-T2) Alexa Fluor® 594

sc-1180 AF594
200 µg/ml
RMB2685.00

CD5 抗体 (UCH-T2) Alexa Fluor® 647

sc-1180 AF647
200 µg/ml
RMB2685.00

CD5 抗体 (UCH-T2) Alexa Fluor® 680

sc-1180 AF680
200 µg/ml
RMB2685.00

CD5 抗体 (UCH-T2) Alexa Fluor® 790

sc-1180 AF790
200 µg/ml
RMB2685.00

How can CD5 (UCH-T2): sc-1180 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: DefinitelyNotMatt
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-1180 PE) or fluoroscein (sc-1180 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Performed well in WB in CCRFPerformed well in WB in CCRF-CEM whole cell lysate. -SCBT QC
Date published: 2015-06-09
Rated 5 out of 5 by from StrongStrong, clear band of interest observed in WB in HuT 78 and MOLT-4 whole cell lysates. -SCBT QC
Date published: 2015-05-25
Rated 5 out of 5 by from GreatGreat, strong band in WB in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-05-10
Rated 5 out of 5 by from Published data for IP and WB in human CLL cellsPublished data for IP and WB in human CLL cells. -SCBT Publication Review
Date published: 2015-04-29
Rated 5 out of 5 by from Good antibody for westernGood antibody for western, clean background and specific band in non-transfected 293T, human CD5 transfected 293T and Jurkat whole cell lysates. - SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Published data for IP and WB in human B cellsPublished data for IP and WB in human B cells. -SCBT Publication Review
Date published: 2015-03-17
Rated 5 out of 5 by from Good specificity and signal strength in IB4Good specificity and signal strength in IB4 whole cell lysate. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Great immunoperoxidase membrane stainingGreat immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded human lymphoma. -SCBT QC
Date published: 2015-01-29
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CD5 抗体 (UCH-T2) is rated 5.0 out of 5 by 9.
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