Date published: 2025-9-8
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cathepsin D Antibody (D-7) - Western Blotting - Image 283620
cathepsin D 抗体 (D-7): sc-377299. SK-BR-3 (A), NIH/3T3 (B), RAW 264. 7 (C) 和 AMJ2-C11 (D) 全细胞裂解液中 cathepsin D 表达的 Western 印迹分析.
cathepsin D 抗体 (D-7): sc-377299. SK-BR-3 (A), NIH/3T3 (B), RAW 264. 7 (C) 和 AMJ2-C11 (D) 全细胞裂解液中 cathepsin D 表达的 Western 印迹分析.

cathepsin D 抗体 (D-7): sc-377299

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说明书
  • cathepsin D 抗体 D-7 是小鼠单克隆 IgM κ，cathepsin D抗体, 在49篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的cathepsin D的C-terminus的氨基酸383-411之间
  • 推荐用于 mouse, rat 和 human 来源的cathepsin D WB, IP, IF, IHC(P) 和 ELISA检测
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取cathepsin D (D-7): sc-377299的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 cathepsin D 抗体 (D-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与cathepsin D 抗体 (D-7) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cathepsin 抗体 包括 cathepsin 6, cathepsin A, cathepsin B, cathepsin C, cathepsin D, cathepsin E, cathepsin H, cathepsin K, cathepsin L和cathepsin O

组织蛋白酶D抗体（D-7）是一种IgM κ小鼠单克隆组织蛋白酶D抗体（也称为CTSD抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的组织蛋白酶D蛋白。组织蛋白酶D抗体（D-7）既可作为非偶联的抗组织蛋白酶D抗体形式，也可作为多种偶联形式的抗组织蛋白酶D抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。组织蛋白酶家族的蛋白水解酶包含几个不同的蛋白酶类。半胱氨酸蛋白酶类包括组织蛋白酶B、L、H、K、S和O。天冬氨酸蛋白酶类由组织蛋白酶D和E组成。组织蛋白酶G属于丝氨酸蛋白酶类。大多数组织蛋白酶都是溶酶体的，每种都参与细胞代谢，参与肽生物合成和蛋白质降解等多种事件。组织蛋白酶还可能裂解一些蛋白质前体，从而释放调节肽。组织蛋白酶D基因的启动子区域包含五个Sp1结合位点和四个AP-2结合位点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

订购信息

产品名称产品编号规格价格数量收藏夹

cathepsin D 抗体 (D-7)

sc-377299
200 µg/ml
RMB2377.00

cathepsin D (D-7): m-IgGκ BP-HRP 套装

sc-550757
200 µg Ab; 40 µg BP
RMB2662.00

cathepsin D 抗体 (D-7) AC

sc-377299 AC
500 µg/ml, 25% agarose
RMB3129.00

cathepsin D 抗体 (D-7) Alexa Fluor® 488

sc-377299 AF488
200 µg/ml
RMB2685.00

cathepsin D 抗体 (D-7) Alexa Fluor® 647

sc-377299 AF647
200 µg/ml
RMB2685.00

cathepsin D 抗体 (D-7) FITC

sc-377299 FITC
200 µg/ml
RMB2482.00

cathepsin D 抗体 (D-7) HRP

sc-377299 HRP
200 µg/ml
RMB2377.00

cathepsin D (D-7) 中和勝肽

sc-377299 P
100 µg/0.5 ml
RMB511.00

cathepsin D 抗体 (D-7) PE

sc-377299 PE
200 µg/ml
RMB2580.00

What is recommendable for a WesternBlot, using cathepsin D Antibody (D-7): sc-377299 and a secondary antibody or use directly cathepsin D Antibody (D-7): sc-377299 conjugated with HRP? Thanks

Asked by: CUES
Thank you for your question. It is by personal preference to reduce the number of steps on your protocol we recommend using the HRP conjugated form. https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2023-08-18

Does it work in pig tissue? Thanks.

Asked by: YMC39
Thank you for your question. We validate this antibody for mouse, rat and human. There is a 78% homology in pig. We only honor our warranty if the homology is 95% or higher.
Answered by: BlakeJ
Date published: 2023-03-30

Does this work by immunofluorescence in mouse cells? Is there a recommended dilution for this application? Thank you.

Asked by: scienceguy
Anti-cathepsin D Antibody (D-7): sc-377299 is recommended for detection of cathepsin D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. For immunofluorescence, the recommended starting dilution is 1:50, with a dilution range between 1:50-1:500.
Answered by: Tech Support Europe
Date published: 2021-08-25

I will use this antibody (sc-377299) in western blot. Can you suggest second antibody (HRP conjugated) for me? Thanks.

Asked by: Researcherman
Thank you for your question. As secondary antibody with HRP conjugate we recommend m-IgGκ BP-HRP: sc-516102
Answered by: Tech Support Europe
Date published: 2021-07-14

What is the best reagent to dilute this antibody for Western Blotting?

Asked by: MZOIA
Thank you for your question. We recommend using our TBS Blotto A: sc-2333 (https://www.scbt.com/scbt/product/tbs-blotto-a) which is 5% nonfat milk + 1X TBS + 0.05% Tween-20. This is the recommended buffer for blocking, as well as primary and secondary antibody incubations for Western blotting.
Answered by: Technical Support
Date published: 2017-11-08

cathepsin D (D-7): sc-377299 is a mouse IgM, can I use an anti-mouse IgG secondary antibody for detection?

Asked by: cjMara
We recommend using an isotype-specific anti-mouse IgM secondary antibody for detection of this mouse IgM primary antibody. Anti-mouse IgG secondary antibodies may work, due to the similar structures of IgG and IgM, but they are not ideally suited.
Answered by: Technical Support
Date published: 2017-02-02
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Rated 3 out of 5 by from This antibosy works with western blottingThis antibody works with western blotting with SAS cells lysate.
Date published: 2020-06-12
Rated 4 out of 5 by from It works wellWe checked this antibody on lysates from immortalized human proximal tubular epithelial cells by Western blot. It detects a single band (around 48-50 kDa, presumably the immature form) with no background signals, after overnight incubation (1:500) at 4°C. We were unable to detect the mature form of Cathepsin D (35 kDa) in these cells upon these experimental conditions.
Date published: 2019-06-12
Rated 5 out of 5 by from works very well for mouse fat tissueI did 1/500 dilution for 4 degree overnight incubation, which gives me expected band, upper band between 40-55 kDa, around 52Kda or 46 kDa, I am not sure. But it also can detect the mouse IgG light chain, the bottom band
Date published: 2017-12-21
Rated 3 out of 5 by from Working antibody in WBThis antibody works ok in WB with human and mouse samples. There are quite a few non-specific bands.
Date published: 2017-10-18
Rated 5 out of 5 by from it's excitingWe made an experiment with cathepsin D antibody and found it very useful. This antibody has very good activity and a demonstrable effect.
Date published: 2017-10-18
Rated 1 out of 5 by from It doesn't work in IF in rat 832/13 ins1 cellsPFA fixed, permeabilized with Triton-X10. Used at 1:50 for IF
Date published: 2017-10-15
Rated 5 out of 5 by from good featuresuse in NB4 cells ,the trips is very abvious,thank you for your free sample
Date published: 2017-09-06
Rated 5 out of 5 by from Works greatWorks great with no background signals on western blot at 1:200 in mouse cell line
Date published: 2017-08-03
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cathepsin D 抗体 (D-7) is rated 4.4 out of 5 by 20.
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