Date published: 2025-9-8
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cathepsin D Antibody (C-5) - Western Blotting - Image 281779
cathepsin D 抗体 (C-5): sc-377124. MCF7 (A) 和 ZR-75-1 (B) 全细胞裂解液中 cathepsin D 表达的 Western 印迹分析.
cathepsin D 抗体 (C-5): sc-377124. MCF7 (A) 和 ZR-75-1 (B) 全细胞裂解液中 cathepsin D 表达的 Western 印迹分析.

cathepsin D 抗体 (C-5): sc-377124

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寫評論提問

说明书
  • cathepsin D抗体(C-5)是小鼠单克隆IgG2b κ, 在26篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的cathepsin D的内部区域内的氨基酸171-189之间
  • 抗-cathepsin D 抗体 (C-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 cathepsin D
  • 抗cathepsin D抗体（C-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cathepsin D (C-5): sc-377124的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 cathepsin D 抗体 (C-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与cathepsin D 抗体 (C-5) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cathepsin 抗体 包括 cathepsin 6, cathepsin A, cathepsin B, cathepsin C, cathepsin D, cathepsin E, cathepsin H, cathepsin K, cathepsin L和cathepsin O

组织蛋白酶D抗体（C-5）是一种IgG2bκ小鼠单克隆组织蛋白酶D抗体（也称为CTSD抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的组织蛋白酶D。组织蛋白酶D抗体（C-5）既可作为非偶联抗组织蛋白酶D抗体形式，也可作为多种偶联形式的抗组织蛋白酶D抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。组织蛋白酶家族的蛋白酶包含几种不同的蛋白酶类。半胱氨酸蛋白酶类包括组织蛋白酶B、L、H、K、S和O。天冬氨酸蛋白酶类由组织蛋白酶D和E组成。组织蛋白酶G属于丝氨酸蛋白酶类。大多数组织蛋白酶都是溶酶体的，每种都参与细胞代谢，参与各种事件，如肽的生物合成和蛋白质降解。组织蛋白酶还可以裂解一些蛋白质前体，从而释放调节肽。组织蛋白酶D基因的启动子区域包含五个Sp1结合位点和四个AP-2结合位点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cathepsin D 抗体 (C-5) 参考文献:

  1. 人肺泡巨噬细胞 cathepsin S（一种弹性蛋白分解半胱氨酸蛋白酶）的分子克隆和表达。  |  Shi, GP., et al. 1992. J Biol Chem. 267: 7258-62. PMID: 1373132
  2. 人类 cathepsin D 基因的分子结构。  |  Redecker, B., et al. 1991. DNA Cell Biol. 10: 423-31. PMID: 2069717
  3. 作为发现新型抗癌药物的有前景靶点的蛋白酶D。  |  Dubey, V. and Luqman, S. 2017. Curr Cancer Drug Targets. 17: 404-422. PMID: 28215160
  4. 重组原-CTSD（凝血酶 D）可增强α-突触核蛋白病模型中 SNCA/α-Synuclein 的降解。  |  Prieto Huarcaya, S., et al. 2022. Autophagy. 18: 1127-1151. PMID: 35287553
  5. 大鼠 cathepsin L 的 cDNA 分子克隆和测序。  |  Ishidoh, K., et al. 1987. FEBS Lett. 223: 69-73. PMID: 3666143
  6. 大鼠 cathepsin H 的 cDNA 分子克隆和测序。  |  Ishidoh, K., et al. 1987. FEBS Lett. 226: 33-7. PMID: 3691815
  7. 通过TGFBI-CCL20信号传导，组织蛋白酶D促进肿瘤相关巨噬细胞的极化和转移。  |  Lee, SG., et al. 2024. Exp Mol Med. 56: 383-394. PMID: 38297161
  8. 对编码大鼠脾脏胰蛋白酶 E 的两个 cDNA 克隆进行分离和测序，并对纯化的胰蛋白酶 E 的活化进行分析。  |  Okamoto, K., et al. 1995. Arch Biochem Biophys. 322: 103-11. PMID: 7574663
  9. 人 cathepsin O（一种新型内切蛋白酶和兔 OC2 的同源物）的分子克隆。  |  Shi, GP., et al. 1995. FEBS Lett. 357: 129-34. PMID: 7805878
  10. Cathepsin B 是一种与转移进展有关的半胱氨酸蛋白酶，在大鼠前列腺和乳腺退化过程中也有表达。  |  Guenette, RS., et al. 1994. Eur J Biochem. 226: 311-21. PMID: 8001549
  11. 小鼠 cathepsin G 基因的分子克隆, 染色体位置和组织特异性表达。  |  Heusel, JW., et al. 1993. Blood. 81: 1614-23. PMID: 8453108
  12. 小鼠 cathepsin K：cDNA 克隆以及该基因在小鼠发育过程中破骨细胞和某些肥大软骨细胞中的主要表达。  |  Rantakokko, J., et al. 1996. FEBS Lett. 393: 307-13. PMID: 8814310

订购信息

产品名称产品编号规格价格数量收藏夹

cathepsin D 抗体 (C-5)

sc-377124
200 µg/ml
RMB2377.00

cathepsin D (C-5): m-IgGκ BP-HRP 套装

sc-523201
200 µg Ab, 40 µg BP
RMB2662.00

cathepsin D 抗体 (C-5) AC

sc-377124 AC
500 µg/ml, 25% agarose
RMB3129.00

cathepsin D 抗体 (C-5) HRP

sc-377124 HRP
200 µg/ml
RMB2377.00

cathepsin D 抗体 (C-5) FITC

sc-377124 FITC
200 µg/ml
RMB2482.00

cathepsin D 抗体 (C-5) PE

sc-377124 PE
200 µg/ml
RMB2580.00

cathepsin D 抗体 (C-5) Alexa Fluor® 488

sc-377124 AF488
200 µg/ml
RMB2685.00

cathepsin D 抗体 (C-5) Alexa Fluor® 546

sc-377124 AF546
200 µg/ml
RMB2685.00

cathepsin D 抗体 (C-5) Alexa Fluor® 594

sc-377124 AF594
200 µg/ml
RMB2685.00

cathepsin D 抗体 (C-5) Alexa Fluor® 647

sc-377124 AF647
200 µg/ml
RMB2685.00

cathepsin D 抗体 (C-5) Alexa Fluor® 680

sc-377124 AF680
200 µg/ml
RMB2685.00

cathepsin D 抗体 (C-5) Alexa Fluor® 790

sc-377124 AF790
200 µg/ml
RMB2685.00

cathepsin D (C-5) 中和勝肽

sc-377124 P
100 µg/0.5 ml
RMB511.00

Can I use sc-377124 with my zebrafish samples?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 1
Date published: 2017-01-26
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Rated 4 out of 5 by from Western BlotWork very nicely for WB in MCF-7 cells treated for 24 hours with estrogen. It needs to be prepared at least 1:500.
Date published: 2023-02-28
Rated 3 out of 5 by from Good but not greatI have tested the sc-377124 antibody in human brain samples. It detects several bands, a strong and clear band at 52KDa, but it was difficult to identify the band at 35KDa, there was a very weak and dirty signal.
Date published: 2022-11-03
Rated 5 out of 5 by from monoclonal antibodes to V-ATPase, CTSC, CTSD, etc.These antibodies work great in WB analysis of mouse liver tissues.
Date published: 2022-01-26
Rated 1 out of 5 by from Couldn't detect anythingI was trying to use your Cathepsin-D (sc-377124/Lot:A0714) in Western Blotting of whole mice heart tissue in 1:1000 dilution. Unfortunately, I could not detect any signal, even though we purchased your more specific m-IgGκ BP-HRP0 (sc-516102/Lot:G1019) secondary antibody (1:10.000 dilution).
Date published: 2020-05-12
Rated 5 out of 5 by from Monoclonal antibody against Cathepsin DThis monoclonal antibody (SC377124) might be (one of) the best available antibody against Cathepsin D. It recognizes the 30-32 kDa heavy chain, it's the mature form of the protein.
Date published: 2019-01-07
Rated 5 out of 5 by from Good Ab for WBI used this Ab for WB on mice liver tissues at 1:50 and it works very well. Bands very clean!
Date published: 2018-04-26
Rated 4 out of 5 by from Good results in WBThis antibody was used for detect the expression of CTSD in Human brain samples.
Date published: 2017-12-15
Rated 4 out of 5 by from work wellDetect 2 bands ~52 and ~35KDa in primary human fibroblasts, blocking 5% milk, dilution 1:400, secondary: m-IgGk BP-HRP: sc-516102 dilution ~1:3000- 1:4000
Date published: 2017-10-24
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cathepsin D 抗体 (C-5) is rated 4.3 out of 5 by 13.
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