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cathepsin D 抗体 (49): sc-136282

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说明书
  • cathepsin D 抗体 49 是小鼠单克隆 IgG2a, 在4篇文献中引用,提供 50 µg/0.5 ml
  • 推荐用于 human 来源的cathepsin D WBIP检测; 不建议用于IP
  • 看 cathepsin D (D-7): sc-377299,可查看有标记的 cathepsin D 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成cathepsin D 抗体 (49)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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组织蛋白酶家族的蛋白水解酶包含几种不同类型的蛋白酶。半胱氨酸蛋白酶类包括组织蛋白酶B、L、H、K、S和O。天冬氨酸蛋白酶类由组织蛋白酶D和E组成。组织蛋白酶G属于丝氨酸蛋白酶类。大多数组织蛋白酶是溶酶体酶,每种酶都参与细胞代谢,参与肽合成和蛋白质降解等各种事件。组织蛋白酶还可以切割一些蛋白质前体,从而释放调节肽。组织蛋白酶D基因的启动子区包含五个Sp1结合位点和四个AP-2结合位点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cathepsin D 抗体 (49) 参考文献:

  1. 炎症性肠病巨噬细胞中,Cathepsin D上调。  |  Hausmann, M., et al. 2004. Clin Exp Immunol. 136: 157-67. PMID: 15030527
  2. 肿瘤疾病恶性进展中的组织蛋白酶D(综述)。  |  Leto, G., et al. 1992. Anticancer Res. 12: 235-40. PMID: 1567172
  3. 组织蛋白酶D的双重作用:配体和蛋白酶。  |  Fusek, M. and Vetvicka, V. 2005. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 149: 43-50. PMID: 16170387
  4. 乳腺癌中的组织蛋白酶D和预后。  |  Tandon, AK., et al. 1990. N Engl J Med. 322: 297-302. PMID: 2296271
  5. 恶性黑色素瘤中组织蛋白酶D的过度表达。  |  Zhu, L., et al. 2013. Fukuoka Igaku Zasshi. 104: 370-5. PMID: 24511668
  6. 足细胞中的蛋白酶D在蛋白尿和慢性肾脏病的发病机理中起着重要作用。  |  Yamamoto-Nonaka, K., et al. 2016. J Am Soc Nephrol. 27: 2685-700. PMID: 26823550
  7. 姜黄素对代谢综合征患者血清组织蛋白酶D的影响。  |  Shirmohammadi, L., et al. 2020. Cardiovasc Hematol Disord Drug Targets. 20: 116-121. PMID: 31538907
  8. 心肌组织蛋白酶D在猝死中的下调。  |  Kakimoto, Y., et al. 2020. PLoS One. 15: e0230375. PMID: 32176724
  9. 鉴定BAG2和Cathepsin D作为帕金森氏病的血浆生物标志物。  |  Kang, J., et al. 2021. Clin Transl Sci. 14: 606-616. PMID: 33202088
  10. 巨噬细胞内体中的膜相关性组织蛋白酶D。  |  Diment, S., et al. 1988. J Biol Chem. 263: 6901-7. PMID: 3360812

订购信息

产品名称产品编号规格价格数量收藏夹

cathepsin D 抗体 (49)

sc-136282
50 µg/0.5 ml
CNY2422.00

How much sample do you recommend loading on the gel for Western blot detection using cathepsin D (49): sc-136282?

Asked by: Trav11
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by LM from Great antibody for Western Blot with a strongGreat antibody for Western Blot with a strong band at the correct molecular weight in Hep G2 whole cell lysate. -SCBT QC
Date published: 2014-07-17
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cathepsin D 抗体 (49) is rated 5.0 out of 5 by 1.
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