Date published: 2025-9-7
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c-Fos Antibody (C-10) - Western Blotting - Image 379667
c-Fos 抗体 (C-10): sc-271243. A-431 (A), Y79 (B), SK-BR-3 (C), C2C12 (D) 和 TK-1 (E) 全细胞裂解液中 c-Fos 表达的 Western 印迹分析.
c-Fos 抗体 (C-10): sc-271243. A-431 (A), Y79 (B), SK-BR-3 (C), C2C12 (D) 和 TK-1 (E) 全细胞裂解液中 c-Fos 表达的 Western 印迹分析.

c-Fos 抗体 (C-10): sc-271243

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寫評論提問

说明书
  • c-Fos抗体(C-10)是小鼠单克隆IgG2a κ, 在71篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的c-Fos的内部区域内的氨基酸120-155之间
  • c-Fos 抗体 (C-10) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的c-Fos, Fos B, Fra-1 and Fra-2; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 可用于ChIP的TransCruz试剂(sc-271243 X, 200 µg/0.1 ml)
  • 抗c-Fos抗体（C-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取c-Fos (C-10): sc-271243的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是c-Fos Antibody (C-10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与c-Fos Antibody (C-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加c-Fos抗体，包括Fos B

c-Fos抗体（C-10）是一种IgG2a κ小鼠单克隆c-Fos抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人的c-Fos、Fos B、Fra-1和Fra-2。c-Fos抗体（C-10）既可作为非偶联抗c-Fos抗体形式提供，也可作为多种偶联形式的抗c-Fos抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。c-Fos癌基因最初是在两个独立的小鼠骨肉瘤病毒分离株和一个禽类肾母细胞瘤病毒中检测到的。其细胞同源物c-Fos编码一种核磷蛋白，该蛋白可被多种因子迅速且瞬时诱导，并作为几个基因的转录调节因子。与形成同源二聚体和异源二聚体并与特定DNA反应元件结合的c-Jun蛋白不同，c-Fos蛋白仅作为与Jun基因家族成员结合的异源二聚体时才具有活性。c-Fos的功能同源物包括Fra-1、Fra-2和Fos B基因。此外，选定的ATF/CREB家族成员可以与Fos和Jun形成亮氨酸拉链二聚体。不同的二聚体对AP-1和CRE位点表现出不同的特异性和亲和力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

c-Fos 抗体 (C-10)

sc-271243
200 µg/ml
RMB2377.00

c-Fos (C-10): m-IgG Fc BP-HRP 套装

sc-525793
200 µg Ab; 10 µg BP
RMB2662.00

c-Fos (C-10): m-IgG2a BP-HRP 套装

sc-546417
200 µg Ab; 10 µg BP
RMB2662.00

c-Fos 抗体 (C-10) X

sc-271243 X
200 µg/0.1 ml
RMB2377.00

c-Fos 抗体 (C-10) AC

sc-271243 AC
500 µg/ml, 25% agarose
RMB3129.00

c-Fos 抗体 (C-10) HRP

sc-271243 HRP
200 µg/ml
RMB2377.00

c-Fos 抗体 (C-10) FITC

sc-271243 FITC
200 µg/ml
RMB2482.00

c-Fos 抗体 (C-10) PE

sc-271243 PE
200 µg/ml
RMB2580.00

c-Fos 抗体 (C-10) Alexa Fluor® 488

sc-271243 AF488
200 µg/ml
RMB2685.00

c-Fos 抗体 (C-10) Alexa Fluor® 546

sc-271243 AF546
200 µg/ml
RMB2685.00

c-Fos 抗体 (C-10) Alexa Fluor® 594

sc-271243 AF594
200 µg/ml
RMB2685.00

c-Fos 抗体 (C-10) Alexa Fluor® 647

sc-271243 AF647
200 µg/ml
RMB2685.00

c-Fos 抗体 (C-10) Alexa Fluor® 680

sc-271243 AF680
200 µg/ml
RMB2685.00

c-Fos 抗体 (C-10) Alexa Fluor® 790

sc-271243 AF790
200 µg/ml
RMB2685.00

c-Fos (C-10) 中和勝肽

sc-271243 P
100 µg/0.5 ml
RMB511.00

Because there is no way to know if it works on mouse tissue until we try. To avoid anti-mouse secondary, it would be nice to try anti-Fos conjugated to Alexa 488 or 594. Do you have small samples of these?

Asked by: Svet
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

How can I get a free sample? I want to test it before making purchases. Thanks!

Asked by: kisong
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-04-29

Is there a mouse-on-mouse issue, when used on mouse tissue?

Asked by: Berry2019
Thank you for your question. There are numerous publications citing use of this mouse monoclonal c-Fos antibody on mouse tissue. Typically, unless your tissues are rich with endogenous IgG there should not be a lot of non-specific binding using a mouse monoclonal antibody on mouse tissue. However, we do offer this antibody directly conjugated, so you can avoid having to use any anti-mouse IgG secondary antibody for detection, and have the most specific result.
Answered by: Technical Support
Date published: 2019-03-15

Hello, I would like to know the price of this product (Is c-Fos (C-10): sc-271243 monoclonal antibody). Thank you!

Asked by: Aline F Melo
Thank you for your question. Please visit our website for up-to-date pricing information: https://www.scbt.com/scbt/product/c-fos-antibody-c-10 ?requestFrom=search
Answered by: Technical Support
Date published: 2019-01-06

What is the expiration date of this ab kept at 4C?

Asked by: nf85
All of our products are warrantied for one year from the date purchased. We do not provide expiration dates for our antibodies. If properly stored at 4C it can continue to be viable for several years if not longer.
Answered by: Technical Support
Date published: 2018-12-21

这个抗体已经买了一年半了，现在还能用吗？

Asked by: wwwwwwwww
感谢您的提问。抱歉这个没法确认，您可以试验试试。更多问题，欢迎通过：电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. Sorry that we can not sure, maybe you can do a test. For more questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-11-01

Is c-Fos (C-10): sc-271243 monoclonal antibody appropriate for use in IHC with paraffin-embedded sections?

Asked by: jenniferc
Thank you for your question. Yes, c-Fos (C-10): sc-271243 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-10
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Rated 5 out of 5 by from Works correctly.We have tested the antibody for IF at a 1/500 concentration and Alexa secondary antibodies at 1/500. In our hands, it works correctly. Images of mice injected with a hypertonic saline solution intraperitoneally.
Date published: 2025-03-05
Rated 4 out of 5 by from mouse monoclonal anti-c-Fosmouse monoclonal anti-c-Fos (sc-271243) Santa Cruz Biotechnology; diluted 1:1000
Date published: 2021-05-11
Rated 4 out of 5 by from Worked for mouse brain tissueI tried c-Fos ab C-10, D-1, E-8 at the same time in PFA-fixed flozen mouse brain and found only C-10 worked well. I applied 1:500 ~ 1:8000 dilution in 4°C, over night for floated 40um thick-sections. 1:8000 dilution was better for me.
Date published: 2021-02-18
Rated 1 out of 5 by from It is not useful for immunohistochemitryWe processed sections of the same subject with different dilutions and with a polyclonal antibody that has resulted very good for our purposes. The monoclonal c-Fos (C-10) did not give a good immunostaining. Moreover, it uncovered structures that look like blood vessels and obstruct a good appreciation of the c-Fos positive cells. It is important to indicate that such vessels were not vissible with our reference antibody.
Date published: 2018-04-27
Rated 5 out of 5 by from Worked great for IFI was looking for a robust c-Fos antibody for immuno-staining in mouse brain tissue. This one worked like a champ. I used 1:300 in PBST, RT o.n. for 100um slice. For secondary, I used Invitrogen isotype specific Alexa 647 A-21241 1:300 in PBST, RT o.n. and other secondary abs yielded nonspecific signals.
Date published: 2018-03-21
Rated 5 out of 5 by from Best SCBT alternative to sc-52We tried every alternative and this is by far the best. Great staining at 1:500.
Date published: 2018-03-14
Rated 5 out of 5 by from excellent almost same as sc-52it worked great on brain sections at 1:500 dilution, I feel better than the previous rabbit poly clonal
Date published: 2017-05-18
Rated 5 out of 5 by from goodsIts specificity for epitope mapping between amino acids 120-155 in the internal region of c-Fos of human origin is very clear and very useful
Date published: 2017-01-17
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c-Fos 抗体 (C-10) is rated 4.3 out of 5 by 14.
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