Date published: 2025-9-8

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c-Fos 抗体 (6-2H-2F): sc-447

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说明书
  • c-Fos抗体(6-2H-2F)是小鼠单克隆IgG1 κ, 在70篇文献中引用,规格为200 µg/ml
  • c-Fos的human起源的氨基酸139-211(亮氨酸拉链)之间的表位图谱
  • 抗-c-Fos 抗体 (6-2H-2F) 推荐用于 WB 和 IP,检测mouse, rat 和human 来源的 c-Fos, Fos B, Fra-1 and Fra-2
  • 抗c-Fos抗体(6-2H-2F)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-447 X, 200 µg/0.1 ml)
  • 关于如何获取c-Fos (6-2H-2F): sc-447免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是c-Fos Antibody (6-2H-2F) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与c-Fos Antibody (6-2H-2F) 打包提供(请参阅下面的订购信息)。

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関連項目

c-Fos抗体(6-2H-2F)是一种小鼠单克隆IgG1 kappa轻链抗体,针对人类c-Fos的氨基酸139-211,该区域包含必需的亮氨酸拉链二聚化结构域。c-Fos小鼠单克隆抗体(6- 2H-2F)可识别亮氨酸拉链区域内的表位,并表现出广泛的物种交叉反应性,可通过蛋白质印迹(WB)和免疫沉淀(IP)检测小鼠、大鼠和人类样本中的c-Fos、FosB、Fra-1和Fra-2蛋白。抗-c-Fos 单克隆抗体(6-2H-2F)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 结合物,可满足各种实验需求。c-Fos的结构组织揭示了通过其亮氨酸拉链结构进行的复杂调节,该结构能够与Jun家族蛋白(如c-Jun)形成特定的异二聚体。这些异二聚体复合物构成了激活蛋白-1(AP-1)转录因子,该因子与特定的DNA反应元件结合,从而控制基因表达。c-Fos是一种核磷蛋白和早期基因,对包括生长因子和应激刺激在内的各种细胞信号作出快速、短暂的反应。通过这种动态调节和基因表达调控能力,c-Fos成为细胞增殖、分化、存活等过程的重要协调者。在病理条件下,c-Fos的结构完整性和正常功能尤为重要,因为失调会导致肿瘤发生。通过这些不同的细胞作用,c-Fos (6-2H-2F)抗体为研究人员提供了研究转录调控和细胞信号传导途径的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

c-Fos 抗体 (6-2H-2F)

sc-447
200 µg/ml
RMB2377.00

c-Fos (6-2H-2F): m-IgG1 BP-HRP 套装

sc-531624
200 µg Ab; 20 µg BP
RMB2662.00

c-Fos 抗体 (6-2H-2F) X

sc-447 X
200 µg/0.1 ml
RMB2377.00

c-Fos 抗体 (6-2H-2F) AC

sc-447 AC
500 µg/ml, 25% agarose
RMB3129.00

c-Fos 抗体 (6-2H-2F) HRP

sc-447 HRP
200 µg/ml
RMB2377.00

c-Fos 抗体 (6-2H-2F) FITC

sc-447 FITC
200 µg/ml
RMB2482.00

c-Fos 抗体 (6-2H-2F) PE

sc-447 PE
200 µg/ml
RMB2580.00

c-Fos 抗体 (6-2H-2F) Alexa Fluor® 488

sc-447 AF488
200 µg/ml
RMB2685.00

c-Fos 抗体 (6-2H-2F) Alexa Fluor® 546

sc-447 AF546
200 µg/ml
RMB2685.00

c-Fos 抗体 (6-2H-2F) Alexa Fluor® 594

sc-447 AF594
200 µg/ml
RMB2685.00

c-Fos 抗体 (6-2H-2F) Alexa Fluor® 647

sc-447 AF647
200 µg/ml
RMB2685.00

c-Fos 抗体 (6-2H-2F) Alexa Fluor® 680

sc-447 AF680
200 µg/ml
RMB2685.00

c-Fos 抗体 (6-2H-2F) Alexa Fluor® 790

sc-447 AF790
200 µg/ml
RMB2685.00

Can this antibody be used in fluorescent immunohistochemistry?

Asked by: alexneuro
We do not recommend this antibody for immunofluorescence.
Answered by: Dallas Tech Service
Date published: 2021-04-07

I don't see additional bands in the datasheet, but it also says "recommended for detection of c-Fos, Fos B, Fra-1 and Fra-2". Does this antibody also detect other fos family proteins FosB, Fra-1, or Fra-2 by western blot, lacking specificity?

Asked by: sacrifice
Thank you for your question. This monoclonal antibody recognizes an epitope that is conserved for these members of the Fos gene family of proteins.
Answered by: Technical Support
Date published: 2019-06-16

I have learned that this antibody can be used to detect c-fos in the monkey. However, can it be used on neuronal tissue using immunohistochemistry (e.g. paraffin-embedded sections)? If not, are there any alternatives?

Asked by: myprefrontal
Thank you for your question. We do not currently recommend this antibody, c-Fos (6-2H-2F): sc-447, for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, or for IHC(P). This antibody is recommended for Western blot and immunoprecipitation. We do offer many other antibodies for c-Fos that are suitable for IHC(P), unfortunately, we are not aware of any publications citing use of those antibodies in monkey. Therefore, we can not recommend them for that species.
Answered by: Technical Support
Date published: 2017-11-02

Can c-Fos (6-2H-2F): sc-447 be frozen?

Asked by: jenniferc
No. The antibody solution contains gelatin. When the gelatin is frozen the antibody precipitates out of solution. However, if an antibody’s datasheet says that the antibody should be stored frozen, then the antibody can be frozen, as these antibodies do not contain gelatin.
Answered by: Technical Support 15
Date published: 2021-02-11
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Rated 1 out of 5 by from no goodno bands detected in Hela cells using 12% gel. The dillution was 1/250.
Date published: 2019-04-01
Rated 5 out of 5 by from 功能出色很多同学都推荐使用,之前课题组师姐也用过,都反馈说效果不错,所以先试着用一下,效果好了继续回购,也可以推荐给更多的同学
Date published: 2018-01-11
Rated 5 out of 5 by from Nice bandRat brain is a appropriate positive control used for this antibody detecion.The specific band is at 62kda by diluted primary antibody1:600 and secondary antibody (sc-516102) 1:4000.
Date published: 2017-07-01
Rated 5 out of 5 by from very goodWe tried this antibody detect this human liver tissue from dilution 1:500, the effect is very good.
Date published: 2017-05-13
Rated 5 out of 5 by from Popular choice for c-FosThe (6-2H-2F) clone is a well established monoclonal antibody featured in over 35 journal articles for a variety of applications including WB, IP, EMSA and ChIP.
Date published: 2016-12-29
Rated 5 out of 5 by from Great antibody for WesternGreat antibody for Western, strong band detected. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Used it for WBUsed it for WB, saw a great band at 62kDa. I'd recommend it for c-Fos anytime. -SCBT QC
Date published: 2014-07-10
Rated 5 out of 5 by from Published with it showing nice IF stainingPublished with it showing nice IF staining on human samples. -SCBT Publication Review
Date published: 2014-03-04
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c-Fos 抗体 (6-2H-2F) is rated 4.6 out of 5 by 10.
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