Date published: 2025-9-7
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BUB1 Antibody (14H5) - Western Blotting - Image 13599
BUB1 抗体 (14H5): sc-47743. K-562 全细胞裂解液中 BUB1 表达的 Western 印迹分析.
BUB1 Antibody (14H5) - Western Blotting - Image 13599
BUB1 抗体 (14H5): sc-47743. K-562 全细胞裂解液中 BUB1 表达的 Western 印迹分析.
BUB1 Antibody (14H5) - Western Blotting - Image 12962
BUB1 抗体 (14H5): sc-47743. HeLa (A) 和 Nocodazole 处理过的 HeLa (B) 全细胞裂解液中 BUB1 表达的 Western 印迹分析.
BUB1 Antibody (14H5) - Western Blotting - Image 31853
BUB1 抗体 (14H5): sc-47743. 非转染: sc-117752 (A) 和人 BUB1 转染: sc-114679 (B) 293T 全细胞裂解液中 BUB1 表达的 Western 印迹分析.
BUB1 抗体 (14H5): sc-47743. K-562 全细胞裂解液中 BUB1 表达的 Western 印迹分析.

BUB1 抗体 (14H5): sc-47743

4.5(4)
寫評論提問

说明书
  • BUB1抗体(14H5)是小鼠单克隆IgG1 κ, 在3篇文献中引用，规格为200 µg/ml
  • 全长重组蛋白human Bub1
  • 推荐用于 human 来源的BUB1 WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是BUB1 Antibody (14H5) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与BUB1 Antibody (14H5) 打包提供（请参阅下面的订购信息）。

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関連項目

额外BUB抗体，包括BUB3

人类细胞中含有两种相关的蛋白激酶，BUB1和BUBR1，它们似乎源自单一的祖先BUB1基因。两种激酶都集中在着丝粒表面附近，监测着丝粒与微管的相互作用。BUB1和BUBR1结合到着丝粒上，被推测是有丝分裂检查点的组成部分，它监测着丝粒的活动，以确定染色体是否已经在纺锤体轴线上达到对齐。BUBR1对正常有丝分裂进程至关重要，因为它可以防止细胞过早进入有丝分裂后期。BUB3是有丝分裂组装复合物的一个保守组分，也参与了早期胚胎发育过程中的关键纺锤体检查点途径。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

BUB1 抗体 (14H5)

sc-47743
200 µg/ml
RMB2377.00

BUB1 (14H5): m-IgG Fc BP-HRP 套装

sc-536805
200 µg Ab; 10 µg BP
RMB2662.00

BUB1 (14H5): m-IgGκ BP-HRP 套装

sc-533970
200 µg Ab; 40 µg BP
RMB2662.00

What is the recommended starting dilution for Western blot for sc-47743: BUB1 (14H5) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from Light bands seen in WB using HeLa and NocodazoleLight bands seen in WB using HeLa and Nocodazole treated HeLa whole cell lysates. -SCBT QC
Date published: 2015-06-11
Rated 5 out of 5 by from Nice band seen in WB using KNice band seen in WB using K-562 whole cell lysate. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from WB publishable data with human HEpWB publishable data with human HEp-2 and KB - SCBT Publication Review
Date published: 2013-04-27
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BUB1 抗体 (14H5) is rated 4.5 out of 5 by 4.
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