Date published: 2025-9-7
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BUB1 Antibody (B-3) - Western Blotting - Image 398983
BUB1 抗体 (B-3): sc-365685. U-2 OS (A), HEL 92. 1. 7 (B), K-562 (C), HeLa (D) 和 F9 (E) 全细胞裂解液中 BUB1 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
BUB1 抗体 (B-3): sc-365685. U-2 OS (A), HEL 92. 1. 7 (B), K-562 (C), HeLa (D) 和 F9 (E) 全细胞裂解液中 BUB1 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

BUB1 抗体 (B-3): sc-365685

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说明书
  • BUB1 抗体 B-3 是小鼠单克隆 IgG3 κ，BUB1抗体, 在10篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列786-1085位于human物种的BUB1的C-terminus
  • 推荐用于 mouse, rat 和 human 来源的BUB1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取BUB1 (B-3): sc-365685的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP和m-IgGκ BP-HRP是BUB1抗体（B-3）在WB and IHC(P)应用中的首选二次检测试剂。这些试剂现在与BUB1 抗体（B-3）捆绑销售（见下文订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 BUB 抗体 包括 BUB3

BUB1抗体（B-3）是一种IgG3 κ小鼠单克隆BUB1抗体（也称为BUB1抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的BUB1蛋白。BUB1抗体（B-3）以非偶联抗BUB1抗体的形式提供。人类细胞包含两种相关的蛋白激酶，BUB1和BUBR1，它们似乎是从单一的祖先BUB1基因进化而来的。这两种激酶都集中在着丝粒表面附近，在那里它们监测着丝粒-微管相互作用。BUB1和BUBR1与着丝粒结合，并被推测为有丝分裂检查点的组成部分，该检查点监测着丝粒活动以确定染色体是否已在纺锤体赤道处实现对齐。BUBR1对于正常的有丝分裂进程至关重要，因为它防止细胞过早进入后期。BUB3是有丝分裂纺锤体组装复合体的保守组成部分，也涉及早期胚胎发育过程中必需的纺锤体检查点通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BUB1 抗体 (B-3) 参考文献:

  1. 人类 BUBR1 是一种有丝分裂检查点激酶，可监测 CENP-E 在着丝点上的功能，并与细胞周期体/APC 结合。  |  Chan, GK., et al. 1999. J Cell Biol. 146: 941-54. PMID: 10477750
  2. BUBR1 磷酸化在有丝分裂检查点激活过程中受到调控。  |  Li, W., et al. 1999. Cell Growth Differ. 10: 769-75. PMID: 10593653
  3. 小鼠 Bub3 基因中断揭示了胚胎早期发育过程中有丝分裂纺锤体检查点的重要功能。  |  Kalitsis, P., et al. 2000. Genes Dev. 14: 2277-82. PMID: 10995385
  4. CENP-E 是体外有丝分裂检查点的重要组成部分。  |  Abrieu, A., et al. 2000. Cell. 102: 817-26. PMID: 11030625
  5. 在暴露于剪切应力的人类内皮细胞中发现一种新基因--SSK1。  |  Donadelli, R., et al. 1998. Biochem Biophys Res Commun. 246: 881-7. PMID: 9618306
  6. hBUB1 和 hBUBR1 激酶在原相期依次组装到动点上，而 hBUBR1 则在有丝分裂期集中在动点板上。  |  Jablonski, SA., et al. 1998. Chromosoma. 107: 386-96. PMID: 9914370

订购信息

产品名称产品编号规格价格数量收藏夹

BUB1 抗体 (B-3)

sc-365685
200 µg/ml
RMB2377.00

BUB1 (B-3): m-IgGκ BP-HRP 套装

sc-522328
200 µg Ab, 40 µg BP
RMB2662.00

BUB1 (B-3): m-IgG3 BP-HRP 套装

sc-550344
200 µg Ab; 40 µg BP
RMB2662.00

Why does your datasheet say that the molecular weight of BUB1 is 150kDa but the Western blots show the protein being about 100kDa. What size band should I be looking for (Human protein)?

Asked by: tasutria
Thank you for your question. The listed molecular weight on the datasheet refers to a publication that cited the size for BUB1 as 150 kDa (PubMedID: 1142067), however published weights vary as low as 110 kDa for this protein (PubMedID: 10704439). The predicted weight based on sequence for BUB1 is ~122 kDa, and it undergoes post-translational modification including multiple phosphorylation sites, which changes it's observed weight on the gel. Our data falls within the observed range of 110-150 kDa. If you have any further questions please contact our Technical Services Department.
Answered by: Technical Service
Date published: 2017-03-01

I am using BUB1 (B-3): sc-365685 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from The antibody doesn't recognise BUB1.I did a siRNA experiment to check if the band that the antibody showed was Bub1 or not and the band didn't disappear. I double checked this with another antibody and the experiment worked properly so it has to be the antibody.
Date published: 2019-04-04
Rated 5 out of 5 by from Great antibody for IF!We received a sample and tested it in human colon carcinoma cells. It works really well in IF. Strong kinetochore signal only with PFA fixation!
Date published: 2017-11-09
Rated 5 out of 5 by from 该抗体对试验帮助很好The specificity of this antibody is very well and good at my research。
Date published: 2017-09-20
Rated 5 out of 5 by from Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-23
Rated 4 out of 5 by from Strong bands seen in WB using HeLa and KStrong bands seen in WB using HeLa and K-562 whole cell lysates. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-01-24
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BUB1 抗体 (B-3) is rated 4.2 out of 5 by 6.
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