Date published: 2025-9-6
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beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (E-3) - Immunohistochemistry - Image 391587
β2-AR 抗体 (E-3) HRP: sc-271322 HRP. 福尔马林固定, 石蜡包埋的人胃组织的直接免疫过氧化物酶染色, 显示腺细胞的胞浆染色. 用 0. 25X UltraCruz® 阻断试剂阻断: sc-516214.
beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (E-3) - Immunohistochemistry - Image 391587
β2-AR 抗体 (E-3) HRP: sc-271322 HRP. 福尔马林固定, 石蜡包埋的人胃组织的直接免疫过氧化物酶染色, 显示腺细胞的胞浆染色. 用 0. 25X UltraCruz® 阻断试剂阻断: sc-516214.
beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (E-3) - Western Blotting - Image 379893
β2-AR 抗体 (E-3): sc-271322. HUV-EC-C 全细胞裂解液中 β2-AR 表达的 Western 印迹分析.
beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (E-3) - Western Blotting - Image 144834
β2-AR (E-3): sc-271322. 非转染: sc-117752 (A) 和人 β2-AR 转染: sc-113753 (B) 293T 全细胞裂解液中 β2-AR 表达的 Western 印迹分析.
β2-AR 抗体 (E-3) HRP: sc-271322 HRP. 福尔马林固定, 石蜡包埋的人胃组织的直接免疫过氧化物酶染色, 显示腺细胞的胞浆染色. 用 0. 25X UltraCruz® 阻断试剂阻断: sc-516214.

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3): sc-271322

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说明书
  • beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 E-3 是小鼠单克隆 IgG2b κ，beta 2 Adrenergic Receptor/ADRB2/β2-AR抗体, 在15篇文献中引用，规格为200 µg/ml
  • 免疫human物种的β2-AR的氨基酸338-413
  • beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 β2-AR
  • 抗beta 2 Adrenergic Receptor/ADRB2/β2-AR抗体（E-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取beta 2 Adrenergic Receptor/ADRB2/β2-AR (E-3): sc-271322的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP是beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (E-3) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (E-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加AR抗体，包括α1A-AR, α1D-AR, α2B-AR, β2-AR和β3-AR

β2-AR抗体（E-3）是一种IgG2b κ小鼠单克隆β2-AR抗体（也称为ADRB2抗体、肾上腺素能β2抗体或B2AR抗体），可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学（P）和酶联免疫吸附试验检测人源β2-AR蛋白。β2-AR抗体（E-3）既有非偶联抗β2-AR抗体形式，也有多种偶联形式的抗β2-AR抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肾上腺素能受体（ARs）（术语"肾上腺素能"反映了肾上腺素的另一种名称）包括四种一般类型（α1、α2、β1和β2），它们存在于不同的靶组织中，对不同的激动剂和拮抗剂具有不同的亲和力和反应。已经分离出了所有主要AR亚型的cDNA克隆，并通过这种方法鉴定出了许多密切相关的受体。已显示每种受体都由单条多肽链组成，该链七次横穿质膜，可能在膜内形成一束螺旋。这些跨膜区域是疏水的，并通过细胞外和细胞内的亲水环相互连接。ARs与特定细胞内效应器的偶联是通过多种异源三聚体G蛋白介导的，并受G蛋白偶联受体激酶（GRKs）、cAMP依赖性蛋白激酶A和蛋白激酶C定向磷酸化的调节。β2肾上腺素能受体与儿茶酚胺（肾上腺素、去甲肾上腺素）结合，影响发育、行为、心脏功能、平滑肌张力和代谢。β2-AR信号复合物可包含C L型钙通道Ca(V)1.2、G蛋白、腺苷酸环化酶、cAMP依赖性激酶和PP2A磷酸酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3)

sc-271322
200 µg/ml
RMB2377.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR (E-3): m-IgG2b BP-HRP 套装

sc-551990
200 µg Ab; 10 µg BP
RMB2662.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) AC

sc-271322 AC
500 µg/ml, 25% agarose
RMB3129.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) HRP

sc-271322 HRP
200 µg/ml
RMB2377.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) FITC

sc-271322 FITC
200 µg/ml
RMB2482.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) PE

sc-271322 PE
200 µg/ml
RMB2580.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) Alexa Fluor® 488

sc-271322 AF488
200 µg/ml
RMB2685.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) Alexa Fluor® 546

sc-271322 AF546
200 µg/ml
RMB2685.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) Alexa Fluor® 594

sc-271322 AF594
200 µg/ml
RMB2685.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) Alexa Fluor® 647

sc-271322 AF647
200 µg/ml
RMB2685.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) Alexa Fluor® 680

sc-271322 AF680
200 µg/ml
RMB2685.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) Alexa Fluor® 790

sc-271322 AF790
200 µg/ml
RMB2685.00

I am planning IHC study for β2- and β3-AR in adrenal medulla. WIth 1/100 dilution, how many immunostained slides could be made by 1 vial (200 µg/ml)?(The size of adrenal tissue section is about 1 cm X 1 cm.) Is the volume of product 1 ml?

Asked by: moonlight
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Can I use 27133 antibody for my mice cell line to do western blot? previously I used Pe conjugated B2AR sc81577 for immunohistochemistry in my mice cell line but it gave me a very diffuse signal. NOw I have to do western please suggest me correct product

Asked by: mubin
Please contact Technical Service for product assistance.
Answered by: Tech Sales
Date published: 2021-04-30

I would like to know the quantity of product. I see the concentration (200ug/ml), but not the volume. Thanks.

Asked by: Timmy
Thank you for your question. Each vial contains 200 µg IgG2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-04-05

Can β2-AR (E-3): sc-271322 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, β2-AR (E-3): sc-271322 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2020-06-23
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Rated 3 out of 5 by from Works for WBWorks moderately well for WB although there are a number of non specific bands.
Date published: 2022-10-03
Rated 5 out of 5 by from 很好用条带还可以，用起来没有什么不好的地方，这个确实需要很多字符才可以，我只能多打几个字。反正就是用起来不错
Date published: 2021-12-21
Rated 1 out of 5 by from Did not perform well for WBI obtained a sample of sc-271322 B2-AR (E-3) [Lot#K2216] to test on human myoblasts transfected with a non-silencing control siRNA or siRNAs specific to ADRB2, the gene that encodes the B2-AR. Immunodetection via western blotting did not produce a clear B2-AR-specific band. In contrast, detection of the same samples using sc-81577 B2-AR (R11E1) worked much more robustly.
Date published: 2017-08-31
Rated 5 out of 5 by from correct bandWB analysis of human liver tissue and got correct band at 1:800 dilution.
Date published: 2017-02-25
Rated 5 out of 5 by from Works WellWe used β2-AR antibody from the first day of our experiment, we bought two antibodies but different batches this time, the quality is stable.
Date published: 2017-02-07
Rated 5 out of 5 by from Satisfactory Western blot data of 2Satisfactory Western blot data of 2-AR expression in non-transfected and human 2-AR transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-12-17
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beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (E-3) is rated 4.0 out of 5 by 6.
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