Date published: 2025-9-6
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beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (R11E1) - Immunohistochemistry - Image 284807
b2-AR (R11E1): sc-81577. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色显示腺细胞亚群的胞浆染色.
beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (R11E1) - Immunohistochemistry - Image 284807
b2-AR (R11E1): sc-81577. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色显示腺细胞亚群的胞浆染色.
beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (R11E1) - Western Blotting - Image 38372
β2-AR (R11E1): sc-81577. 非转染: sc-117752 (A) 和人 β2-AR 转染: sc-112395 (B) 293T 全细胞裂解液中 β2-AR 表达的 Western 印迹分析.
b2-AR (R11E1): sc-81577. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色显示腺细胞亚群的胞浆染色.

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1): sc-81577

3.8(8)
寫評論提問

说明书
  • beta 2 Adrenergic Receptor/ADRB2/β2-AR抗体(R11E1)是小鼠单克隆IgG1 κ, 在17篇文献中引用，规格为200 µg/ml
  • 针对human起源的纯化完整β2-肾上腺素能受体
  • beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) 推荐用于WB, IP, IF, IHC(P) 和 FCM，检测human 来源的 β2-AR
  • 抗beta 2 Adrenergic Receptor/ADRB2/β2-AR抗体（R11E1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取beta 2 Adrenergic Receptor/ADRB2/β2-AR (R11E1): sc-81577的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 AR 抗体包括 α1A-AR, α1D-AR, α2B-AR, β2-AR 和 β3-AR

β2-AR抗体（R11E1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FC M）。β2-AR抗体（R11E1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足不同实验需求。β2-AR在调节儿茶酚胺（如肾上腺素和去甲肾上腺素）的生理效应方面发挥着关键作用，而儿茶酚胺对于调节包括心脏功能、平滑肌松弛和新陈代谢在内的多种过程至关重要。β2-AR主要位于各种细胞类型的质膜上，是一种G蛋白偶联受体，通过异三聚体G蛋白传递信号，激活腺苷酸环化酶，从而增加环磷酸腺苷水平。这种信号传导途径对于调节心率和收缩力以及支气管平滑肌的松弛至关重要，因此β2-AR成为治疗哮喘和心力衰竭等疾病的重要靶点。β2-AR的结构复杂，由七个跨膜螺旋构成，能够与特定的配体结合并激活，并通过翻译后修饰以及与各种细胞内信号传导伙伴的相互作用进行微调，确保对激素刺激做出精确的生理反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) 参考文献:

  1. 从配体结合到基因表达：G 蛋白偶联受体调控的新见解。  |  Collins, S., et al. 1992. Trends Biochem Sci. 17: 37-9. PMID: 1316650
  2. 研究七跨膜节受体的模型系统。  |  Dohlman, HG., et al. 1991. Annu Rev Biochem. 60: 653-88. PMID: 1652922
  3. 通过调节受体基因表达来调节肾上腺素能受体的反应性。  |  Collins, S., et al. 1991. Annu Rev Physiol. 53: 497-508. PMID: 2042970
  4. 药物受体的新生物学。  |  Lefkowitz, RJ., et al. 1989. Biochem Pharmacol. 38: 2941-8. PMID: 2551299
  5. β2-AR信号控制曲妥珠单抗抗药性依赖性途径  |  Liu, D., et al. 2016. Oncogene. 35: 47-58. PMID: 25798840
  6. β2-AR刺激驱动人类棕色脂肪细胞产热  |  Blondin, DP., et al. 2020. Cell Metab. 32: 287-300.e7. PMID: 32755608
  7. 慢性应激通过β2-AR/CREB1信号通路促进糖酵解，从而促进结直肠癌的发展。  |  Guan, Y., et al. 2023. Int J Biol Sci. 19: 2006-2019. PMID: 37151872
  8. 巨噬细胞β2-AR激活通过cAMP/PKA/CREB途径上调Trem1，从而加剧伤口愈合过程中的炎症。  |  Wei, H., et al. 2024. Int Immunopharmacol. 128: 111463. PMID: 38190789
  9. 奥洛德特罗通过调节β2-AR/cAMP/PKA信号通路促进棕色脂肪细胞产热  |  Wang, L., et al. 2024. Biochem Biophys Res Commun. 703: 149689. PMID: 38382361
  10. 研究 G 蛋白偶联受体激酶的一种方法：体外纯化膜测定揭示了不同受体的特异性和 G beta gamma 亚基的调控。  |  Pei, G., et al. 1994. Proc Natl Acad Sci U S A. 91: 3633-6. PMID: 8170959
  11. β-肾上腺素能受体激酶的功能活性靶向结构域：G β gamma 介导的 II 型腺苷酸环化酶刺激抑制剂。  |  Inglese, J., et al. 1994. Proc Natl Acad Sci U S A. 91: 3637-41. PMID: 8170960
  12. 关于 beta 2-肾上腺素受体分子结构和调节的最新观点。  |  Collins, S. 1993. Life Sci. 52: 2083-91. PMID: 8389951

订购信息

产品名称产品编号规格价格数量收藏夹

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1)

sc-81577
200 µg/ml
RMB2377.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR (R11E1): m-IgG Fc BP-HRP 套装

sc-528702
200 µg Ab; 10 µg BP
RMB2662.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR (R11E1): m-IgGκ BP-HRP 套装

sc-521170
200 µg Ab, 40 µg BP
RMB2662.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR (R11E1): m-IgG1 BP-HRP 套装

sc-543073
200 µg Ab; 20 µg BP
RMB2662.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) AC

sc-81577 AC
500 µg/ml, 25% agarose
RMB3129.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) HRP

sc-81577 HRP
200 µg/ml
RMB2377.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) FITC

sc-81577 FITC
200 µg/ml
RMB2482.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) PE

sc-81577 PE
200 µg/ml
RMB2580.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) Alexa Fluor® 488

sc-81577 AF488
200 µg/ml
RMB2685.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) Alexa Fluor® 546

sc-81577 AF546
200 µg/ml
RMB2685.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) Alexa Fluor® 594

sc-81577 AF594
200 µg/ml
RMB2685.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) Alexa Fluor® 647

sc-81577 AF647
200 µg/ml
RMB2685.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) Alexa Fluor® 680

sc-81577 AF680
200 µg/ml
RMB2685.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) Alexa Fluor® 790

sc-81577 AF790
200 µg/ml
RMB2685.00

beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) AC sc-81577 AC 500 µg/ml, 25% agarose 请问这款 81577AC ➕25%agarose是怎么使用的，是直接可以ip吗。里面的agarose什么作用

Asked by: drzzzz
Thanks for your question. Here is link for IP protocol: https://www.scbt.com/zh/resources/protocols/immunoprecipitation-western-blots. 感谢您的提问。免疫沉淀的实验链接详见：https://www.scbt.com/zh/resources/protocols/immunoprecipitation-western-blots。
Answered by: Yanru Tao
Date published: 2022-09-21

I would like to know if the B2-AR (R11E1): sc-81577 antibody contains the entire heavy chain + kappa light chain. I need the Fc region for proximity ligation assays. Thank you.

Asked by: KNMM
Thank you for your question. All our monoclonal antibodies are complete antibodies. They all contain two heavy chains and two light chains. We specify which type of light chain the antibody contains (kappa or lambda) for each antibody. This antibody has kappa light chains.
Answered by: Tech Service
Date published: 2019-06-24

Hi, What is the binding region of this antibody? Does it bind to extracellular part of the receptor? Thank you

Asked by: Kazzz
Thank you for your question. This antibody is specific for Lamin A/C. It was raised against purified, intact β2-AR of human origin, the exact epitope has not been mapped. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-04-30

貴社のβ2-AR Antibody (R11E1)でリンパ球を染め,FMC解析をする場合、生細胞そのままで染色できますか？

Asked by: Yuetsu
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

Can β2-AR (R11E1): sc-81577 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, β2-AR (R11E1): sc-81577 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from Does not IP endogeous or overexpressibg B2ARThis antibody did not immunoprecipitate the receptor In HEK293 cells that transiently overexpress it or in others that are know to endogenously express it to high levels.
Date published: 2018-09-17
Rated 5 out of 5 by from Good detectionI received this antibody as a free sample to test it in A204 cells
Date published: 2018-03-23
Rated 5 out of 5 by from Worked great for WBI obtained a sample of sc-81577 B2-AR (R11E1) [Lot#G1117] to test on human myoblasts transfected with a non-silencing control siRNA or siRNAs specific to ADRB2, the gene that encodes the B2-AR. Immunodetection via western blotting produced a clear B2-AR-specific band. In contrast, detection of the same samples using sc-271322 B2-AR (E-3) worked much less robustly.
Date published: 2017-08-31
Rated 5 out of 5 by from Excellent for IHC!This antibody is great for IHC on paraffin embedded tissue sections, strong and clean staining.
Date published: 2017-01-26
Rated 4 out of 5 by from Publishable data obtained by WB and coPublishable data obtained by WB and co-IP using human HEK293 cells. -SCBT Publication Review
Date published: 2015-05-12
Rated 4 out of 5 by from Paraformaldehyde fixed rabbit NPE cells analyzedParaformaldehyde fixed rabbit NPE cells analyzed with product sc-81577 in IF. -SCBT Publication Review
Date published: 2015-04-22
Rated 3 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the 2-AR protein via Western Blot analysis. -SCBT QC
Date published: 2015-04-11
Rated 3 out of 5 by from Produced nice immunoperoxidase cytoplasmicProduced nice immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-01-23
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beta 2 Adrenergic Receptor/ADRB2/β2-AR 抗体 (R11E1) is rated 3.8 out of 5 by 8.
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