beta-catenin 抗体 (G-10): sc-376959

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Thank you very much for your inquiry. The human β-catenin full-length protein consists of 781 amino acids with an expected molecular weight around 92kDa to be observed in a Western Blot. The protein migration in the gel will be affected by different post-translational modifications, including phosphorylation, so that the actual band size observed may differ from the predicted size. Also a doublet or multiple bands might be observed occasionally depending on the Sample material and conditions used to run the actual gel. The shown doublet band might represent a phosphorylation but this was not further analyzed. In order to do this one could use a combined IP/WB approach with phospho-specific antibodies against β-catenin p-lated Tyr 654 or Tyr 86 or Ser 33/37 and of course against p-lated amino acids such as e.g. P-Tyr PY99 sc-7020. Please contact our technical support for additional information.