Date published: 2025-9-10

021-6093-6350

SCBT Portrait Logo
Seach Input

B7-2 抗体 (GL1): sc-52448

5.0(3)
寫評論提問

说明书
  • B7-2 抗体 GL1 是大鼠单克隆 IgG2a, 在2篇文献中引用,提供 200 µg/ml
  • 针对mouse来源的LPS激活的B细胞
  • 推荐用于 mouse, rat 和 human 来源的B7-2 WB, IP, IF 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 B7-2 (D-6): sc-28347,可查看有标记的 B7-2 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成B7-2 抗体 (GL1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

関連項目

T细胞增殖和淋巴因子产生是由抗原占据TCR引发的,随后由抗原呈递细胞上表达的配体传递共刺激信号。抗原呈递细胞上表达的B7相关细胞表面蛋白B7-1(CD80)和B7-2(CD86)与同源T细胞受体CD28和CTLA-4(细胞毒性T淋巴细胞相关蛋白-4)结合,并引发共刺激信号,以实现最佳的T细胞活化。CTLA-4与CD28共有31%的氨基酸同一性,并且有人提出CD28和CTLA-4在功能上是多余的。SLAM是T细胞上的一种新型受体,当接合时,它以CD28非依赖的方式增强T细胞扩增。B7,也称为BB1,是抗原呈递细胞上表达的CD28和CTLA-4的另一种配体或反受体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

B7-2 抗体 (GL1) 参考文献:

  1. B细胞受体和β2-肾上腺素能受体诱导B细胞中B7-2(CD86)的表达。  |  Kohm, AP., et al. 2002. J Immunol. 168: 6314-22. PMID: 12055247
  2. B7-1和B7-2的不同细胞表面低聚状态:对信号传导的影响。  |  Bhatia, S., et al. 2005. Proc Natl Acad Sci U S A. 102: 15569-74. PMID: 16221763
  3. 泊马度胺通过上调 ICAM-1 和 B7-2 恢复对原发性渗出淋巴瘤的免疫识别。  |  Shrestha, P., et al. 2021. PLoS Pathog. 17: e1009091. PMID: 33411730
  4. 表达B7-1或B7-2共刺激分子的重组牛痘病毒诱导抗肿瘤免疫。  |  Hodge, JW., et al. 1994. Cancer Res. 54: 5552-5. PMID: 7522961
  5. B7-1和B7-2共刺激分子对Th1/Th2发育途径的激活作用不同:在自身免疫性疾病治疗中的应用。  |  Kuchroo, VK., et al. 1995. Cell. 80: 707-18. PMID: 7534215
  6. CD28配体CD80(B7-1)和CD86(B7-2)可诱导CD4+ T细胞长期自发性生长,并在体外诱导类似模式的细胞因子分泌。  |  Levine, BL., et al. 1995. Int Immunol. 7: 891-904. PMID: 7577797
  7. 细胞因子对T辅助细胞表型发育的影响,对人类单核细胞中B7-1和B7-2同型表达的差异调节。  |  Creery, WD., et al. 1996. Eur J Immunol. 26: 1273-7. PMID: 8647204
  8. 对培养的小鼠小胶质细胞中B7-1和B7-2共刺激配体的分析:干扰素-γ和脂多糖上调,白细胞介素-10、前列腺素E2和环磷酸腺苷升高剂下调。  |  Menèndez Iglesias, B., et al. 1997. J Neuroimmunol. 72: 83-93. PMID: 9003248
  9. 人类胃上皮细胞中B7-1和B7-2共刺激分子的表达:在幽门螺杆菌感染期间对CD4+ T细胞活化的潜在作用。  |  Ye, G., et al. 1997. J Clin Invest. 99: 1628-36. PMID: 9120006
  10. 人类炎症性肠病(IBD)中粘膜巨噬细胞亚群上共刺激分子B7.1(CD80)和B7.2(CD86)的差异分布。  |  Rugtveit, J., et al. 1997. Clin Exp Immunol. 110: 104-13. PMID: 9353156

订购信息

产品名称产品编号规格价格数量收藏夹

B7-2 抗体 (GL1)

sc-52448
200 µg/ml
RMB2377.00

B7-2 抗体 (GL1) FITC

sc-52448 FITC
200 µg/ml
RMB2482.00

B7-2 抗体 (GL1) PE

sc-52448 PE
200 µg/ml
RMB2580.00

Is a positive control for the sc-52448 ab available? Just to test if the staining procedure works?

Asked by: Anonymous
Thank you for your question. Recommended positive controls are B7-2 (m): 293T Lysate: sc-118657, RAW 264.7 whole cell lysate: sc-2211 or mouse spleen extract: sc-2391.
Answered by: Tech Support Europe
Date published: 2023-11-24

How can B7-2 (GL1): sc-52448 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: cjMara
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-52448 PE) or fluoroscein (sc-52448 FITC).
Answered by: Technical Support
Date published: 2017-02-25
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_52448, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 111ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Large amount of signal observed in WB in mouse B7Large amount of signal observed in WB in mouse B7-2 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-07-14
Rated 5 out of 5 by from GreatGreat, strong band in WB in RAW 264.7 and I-11.15 whole cell lysates and mouse spleen tissue extract. -SCBT QC
Date published: 2015-02-07
Rated 5 out of 5 by from Nice indirect FCM data of mouse peripheralNice indirect FCM data of mouse peripheral blood leukocytes stained with B7-2 . -SCBT QC
Date published: 2013-11-27
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_zh_CN, sid_52448, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT
B7-2 抗体 (GL1) is rated 5.0 out of 5 by 3.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_zh_CN, sid_52448, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 100ms
  • REVIEWS, PRODUCT