Date published: 2025-9-8
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B7-2 Antibody (D-6) - Western Blotting - Image 380841
B7-2 抗体 (D-6): sc-28347. 在 Raji (A), Ramos (B), WEHI-231 (C), RAW 264. 7 (D) 和 WR19L (E) 全细胞裂解液中 B7-2 表达的 Western 印迹分析.
B7-2 抗体 (D-6): sc-28347. 在 Raji (A), Ramos (B), WEHI-231 (C), RAW 264. 7 (D) 和 WR19L (E) 全细胞裂解液中 B7-2 表达的 Western 印迹分析.

B7-2 抗体 (D-6): sc-28347

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说明书
  • B7-2 抗体 D-6 是小鼠单克隆 IgG1 κ，B7-2抗体, 在64篇文献中引用，规格为200 µg/ml
  • 免疫human物种的B7-2的氨基酸24-223
  • B7-2 抗体 (D-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 B7-2
  • 抗B7-2抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取B7-2 (D-6): sc-28347的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 B7-2 抗体 (D-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与B7-2 抗体 (D-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

B7-2抗体（D-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的B7-2蛋白。B7-2 (D-6) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。B7-2 也称为 CD86，通过提供激活 T 细胞所需的必要共刺激信号，在免疫反应中发挥关键作用。B7-2主要位于抗原呈递细胞上，与T细胞受体（如CD28和CTLA-4）相互作用，促进T细胞活化和增殖。这种相互作用确保T细胞受到适当的刺激，从而对病原体作出反应。B7-2调节免疫耐受并影响免疫反应结果，因此成为癌症和自身免疫性疾病治疗干预的重要靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

B7-2 抗体 (D-6) 参考文献:

  1. 汞诱导的自身免疫需要B7-1和B7-2共刺激分子。  |  Bagenstose, LM., et al. 2002. Clin Exp Immunol. 127: 12-9. PMID: 11882027
  2. B7-1（CD80）和B7-2（CD 86）在人体管状上皮细胞中的体内和体外表达。  |  Niemann-Masanek, U., et al. 2002. Nephron. 92: 542-56. PMID: 12372936
  3. T 淋巴细胞的成本刺激：CD28, CTLA-4 和 B7/BB1 在白细胞介素-2 产物和免疫疗法中的作用。  |  Schwartz, RH. 1992. Cell. 71: 1065-8. PMID: 1335362
  4. 人类 B 淋巴细胞活化抗原 B7 的鼠同源物的结构, 表达和 T 细胞激动活性。  |  Freeman, GJ., et al. 1991. J Exp Med. 174: 625-31. PMID: 1714935
  5. 弓形虫通过激活JNK信号转导诱导B7-2表达。  |  Morgado, P., et al. 2011. Infect Immun. 79: 4401-12. PMID: 21911468
  6. B7 和 CD28 受体家族在自身免疫和免疫逃避中的潜在作用。  |  Harlan, DM., et al. 1995. Clin Immunol Immunopathol. 75: 99-111. PMID: 7535672
  7. 鉴定与 CD28 和 CTLA4 的功能性相互作用有关的 CD80 (B7-1) V 结构域残基。  |  Fargeas, CA., et al. 1995. J Exp Med. 182: 667-75. PMID: 7544393
  8. CD80 的两个细胞外免疫球蛋白样结构域都含有对结合 T 细胞表面受体 CTLA-4 和 CD28 至关重要的残基。  |  Peach, RJ., et al. 1995. J Biol Chem. 270: 21181-7. PMID: 7545666
  9. 一种参与 T 细胞活化的新型受体。  |  Cocks, BG., et al. 1995. Nature. 376: 260-3. PMID: 7617038
  10. CTLA4 介导人类 T 细胞的抗原特异性凋亡。  |  Gribben, JG., et al. 1995. Proc Natl Acad Sci U S A. 92: 811-5. PMID: 7846057
  11. B7-1和B7-2在诱导针对不同小鼠肿瘤的保护性和治疗性抗肿瘤免疫方面具有异质效应。  |  Martin-Fontecha, A., et al. 1996. Eur J Immunol. 26: 1851-9. PMID: 8765031
  12. B7-1和B7-2在免疫球蛋白类转换和生发中心形成中具有重叠的关键作用。  |  Borriello, F., et al. 1997. Immunity. 6: 303-13. PMID: 9075931

订购信息

产品名称产品编号规格价格数量收藏夹

B7-2 抗体 (D-6)

sc-28347
200 µg/ml
RMB2377.00

B7-2 (D-6): m-IgG Fc BP-HRP 套装

sc-528430
200 µg Ab; 10 µg BP
RMB2662.00

B7-2 (D-6): m-IgGκ BP-HRP 套装

sc-520805
200 µg Ab, 40 µg BP
RMB2662.00

B7-2 (D-6): m-IgG1 BP-HRP 套装

sc-542930
200 µg Ab; 20 µg BP
RMB2662.00

B7-2 抗体 (D-6) AC

sc-28347 AC
500 µg/ml, 25% agarose
RMB3129.00

B7-2 抗体 (D-6) HRP

sc-28347 HRP
200 µg/ml
RMB2377.00

B7-2 抗体 (D-6) FITC

sc-28347 FITC
200 µg/ml
RMB2482.00

B7-2 抗体 (D-6) PE

sc-28347 PE
200 µg/ml
RMB2580.00

B7-2 抗体 (D-6) Alexa Fluor® 488

sc-28347 AF488
200 µg/ml
RMB2685.00

B7-2 抗体 (D-6) Alexa Fluor® 546

sc-28347 AF546
200 µg/ml
RMB2685.00

B7-2 抗体 (D-6) Alexa Fluor® 594

sc-28347 AF594
200 µg/ml
RMB2685.00

B7-2 抗体 (D-6) Alexa Fluor® 647

sc-28347 AF647
200 µg/ml
RMB2685.00

B7-2 抗体 (D-6) Alexa Fluor® 680

sc-28347 AF680
200 µg/ml
RMB2685.00

B7-2 抗体 (D-6) Alexa Fluor® 790

sc-28347 AF790
200 µg/ml
RMB2685.00

您好! B7-2抗体 (D-6)是否可以用于小鼠肝脏石蜡切片的免疫荧光染色？

Asked by: Lydia77
感谢您的提问。抗-B7-2 抗体 (D-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 B7-2。支持做小鼠来源的石蜡切片的样本。但对于具体样本是否表达该蛋白，您需要自己查询。您可以拨打电话：021-60936350，发送邮件到[email protected]，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。 Thank you for your question. Anti-B7-2 Antibody (D-6) is recommended for detection of B7-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.It can be used to the sample of paraffin section from mouse. However, you need to query whether specific samples express this protein. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Jiajun Z
Date published: 2022-10-08

可以做western blot吗？一直做不出来。。。

Asked by: 南通大学微微
Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2020-06-03

您好! B7-2抗体 (D-6)是否可以用于石蜡切片的其他脏器组织？比如：肾脏？有没有相关的参考文献?期待您的回复！

Asked by: 南通大学微微
Thank you for your question.We do warranty B7-2(D-6) for IHC-P application.We also can send you a 10ug sample.Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody. 感谢您的提问。我们推荐 B7-2(D-6)通过IHC 实验方法检测石蜡包埋样本，并且可以提供质保。同时，我们可以赠送10ug试用装，检测您的实验样本。试用装申请，请联系:亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-10-22

Hello, Could I possibly receive 10 ug of free sample of B7-2 Antibody (D-6): sc-28347? Thank you very much in advance

Asked by: MDS1989
Thank you for your sample request. Please contact our local distributor in Denmark, AH Diagnostics. They would be glad to send you any samples you wish to try. Their contact information is: AH diagnostics as Phone: +45-8745-9010 Email: [email protected]
Answered by: Tech Service
Date published: 2017-06-08

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-28347 PE), fluorescein (sc-28347 FITC), Alexa Fluor® 488 (sc-28347 AF488) or Alexa Fluor® 647 (sc-28347 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Service 11
Date published: 2017-01-10
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Rated 2 out of 5 by from Not suitable to detect macrophages in FFPEIn my opinion this antibody doesn't work on FFPE, but is ok in immunocytochemistry on cell preparation.
Date published: 2023-12-12
Rated 5 out of 5 by from B7-2 (D-6) is very good for western-blotI used B7-2 (D-6) (sc-28347) to evaluate the phenotype of human monocyte/macrophage by western-blot. According to my results, this antibody is very good, the efficiency was high, specificity was good, and the bands were very clear and strong. Very good experience at Santa Cruz Biotechnology!
Date published: 2022-09-02
Rated 5 out of 5 by from B7-2 NOS2 IFB7-2 NOS2抗体试染C57小鼠， 1：50-1：100浓度， 免疫荧光，很好用，4℃保存很方便…………
Date published: 2022-07-04
Rated 4 out of 5 by from Buon funzionamento!L'Ab ha funzionato bene su più di un lisato da PBMC caricando 60 microgram e incubando l'Ab overnight a 4°C con una concentrazione di 1/100.
Date published: 2020-12-18
Rated 4 out of 5 by from Good signal in IHC-PSpecific signal on paraffin-embedded sections, working dilution (
Date published: 2019-03-29
Rated 5 out of 5 by from Good antibodyThis antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 5 out of 5 by from clear bandwe used this sample to do the western blot ,the sample is the Mouse monoclonal antibody .we used this sample to dectect the B7-2 of RAW246.7 cell.we detected that the band of B7-2 was approximately 66 kDa .
Date published: 2017-10-08
Rated 5 out of 5 by from Great ResultsI used this antibody with MSCs to do western Blot , at a concentration 1:500 it gives nice clear bands
Date published: 2017-09-29
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B7-2 抗体 (D-6) is rated 4.6 out of 5 by 21.
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