Date published: 2025-9-7

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AMPKβ2 抗体 (362-7): sc-100358

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说明书
  • AMPKβ2抗体(362-7)是小鼠单克隆IgG2b (kappa轻链),规格为100 µg/ml
  • 免疫human物种的重组AMPKβ2
  • 推荐用于 mouse, rat 和 human 来源的AMPKβ2 WB, IP 和 ELISA检测
  • 目前,我们还没有完成AMPKβ2 抗体 (362-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    AMPKβ2抗体(362-7)是一种小鼠单克隆IgG2b抗体,可通过应用如蛋白质印迹(WB)、免疫沉淀(IP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类样本中的AMPKβ2。AMPKβ2是5'-AMP激活蛋白激酶(AMPK)复合物的重要调节亚基,在细胞能量稳态中起着至关重要的作用。AMPKβ2主要位于细胞质中,对于AMPK的正常运作至关重要,因为AMPKβ2有助于稳定复合物,并促进激活以应对能量压力。AMPKβ2在细胞质中的存在使其能够快速响应细胞能量水平的波动,使细胞能够相应地适应代谢途径。通过促进参与脂质和葡萄糖代谢的关键酶的磷酸化,AMPKβ2有助于调节能量平衡,使AMPKβ2成为代谢紊乱(如肥胖和糖尿病)的重要参与者。AMPKβ2由272个氨基酸组成,与催化α亚基和γ亚基相互作用,形成激酶活性所必需的异源三聚体复合物。AMPKβ2能够调节能量消耗和代谢过程,这凸显了它在维持细胞健康和应对代谢挑战方面的重要性。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    AMPKβ2 抗体 (362-7) 参考文献:

    1. AMP激活蛋白激酶α2-异构体在心脏对无血流缺血的代谢反应中的作用  |  Zarrinpashneh, E., et al. 2006. Am J Physiol Heart Circ Physiol. 291: H2875-83. PMID: 16877552
    2. 通过 ADIPOR1-AMPK 途径控制正常大鼠和糖尿病大鼠肾远端小管中的糖原合酶。  |  Cammisotto, PG., et al. 2008. Am J Physiol Renal Physiol. 294: F881-9. PMID: 18256313
    3. AMPKβ2 亚基的 Sumoylation 可增强 AMP 激活蛋白激酶的活性。  |  Rubio, T., et al. 2013. Mol Biol Cell. 24: 1801-11, S1-4. PMID: 23552691
    4. AMPKβ 亚基:在 AMPK 复合物的形成过程中不仅仅是一个支架。  |  Sanz, P., et al. 2013. FEBS J. 280: 3723-33. PMID: 23721051
    5. 含有β2调节亚基的AMP激活蛋白激酶复合物在脂肪生成过程中上调,并促进脂肪生成。  |  Katwan, OJ., et al. 2019. Biochem J. 476: 1725-1740. PMID: 31189568
    6. 尼可刹米能独立于 AMP 激活含有 β2 亚基的 AMP 激活蛋白激酶复合物。  |  Suzuki, T., et al. 2020. Biochem Biophys Res Commun. 533: 758-763. PMID: 32993962
    7. TNF 诱导的坏死通过 RIPK3 依赖性 AMPK 激活启动早期自噬事件,但抑制晚期自噬。  |  Wu, W., et al. 2021. Autophagy. 17: 3992-4009. PMID: 33779513
    8. 2 型糖尿病患者骨骼肌细胞的特异性差异。  |  Frankenberg, NT., et al. 2022. Cell Mol Life Sci. 79: 256. PMID: 35460430
    9. AMP 激活的蛋白激酶 β1 或 β2 缺失可促进结肠癌细胞的生长和肿瘤发生。  |  Shi, F., et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54: 1140-1147. PMID: 35880569
    10. 抑制神经元 AMPKβ2 同工酶会损害识别记忆和突触可塑性。  |  Swift, NA., et al. 2024. Neurobiol Dis. 201: 106664. PMID: 39278510

    订购信息

    产品名称产品编号规格价格数量收藏夹

    AMPKβ2 抗体 (362-7)

    sc-100358
    100 µg/ml
    $333.00

    For Western Blot, is it recommended to use denatured or non-denatured conditions with AMPKβ2 (362-7): sc-100358 antibody?

    Asked by: jenniferc
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-03-27
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    Rated 4 out of 5 by from ClearClear, specific band in WB in HeLa whole cell lysate. -SCBT QC
    Date published: 2015-05-13
    Rated 4 out of 5 by from Good specificity in WB in human AMPK 2 transfectedGood specificity in WB in human AMPK 2 transfected 293T whole cell lysates. -SCBT QC
    Date published: 2013-01-06
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    AMPKβ2 抗体 (362-7) is rated 4.0 out of 5 by 2.
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