Date published: 2025-9-6
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AMPK alpha 1 Antibody (H-4) - Western Blotting - Image 309650
AMPKα1 (H-4): sc-398861. IB4 (A), MCF7 (B), Jurkat (C), HeLa (D) 和 K-562 (E) 全细胞裂解液中 AMPKα1 表达的 Western 印迹分析.
AMPKα1 (H-4): sc-398861. IB4 (A), MCF7 (B), Jurkat (C), HeLa (D) 和 K-562 (E) 全细胞裂解液中 AMPKα1 表达的 Western 印迹分析.

AMPK alpha 1 抗体 (H-4): sc-398861

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寫評論提問

说明书
  • AMPK alpha 1 抗体 H-4 是小鼠单克隆 IgG2b κ，AMPK alpha 1抗体, 在45篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的AMPKα1的C-terminus的氨基酸509-533之间
  • AMPK alpha 1 抗体 (H-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 AMPKα1
  • 抗AMPK alpha 1抗体（H-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取AMPK alpha 1 (H-4): sc-398861的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 AMPK alpha 1 抗体 (H-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与AMPK alpha 1 抗体 (H-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 AMPK 抗体 包括 AMPK α 1, AMPK α 2, AMPK β1, AMPK β2和AMPK γ 2

AMPKα1抗体（H-4）是一种IgG2b κ小鼠单克隆AMPK alpha 1抗体（也称为AMPK alpha抗体或PRKAA1抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的AMPK alpha 1蛋白。AMPKα1抗体（H-4）既可作为非共轭抗AMPK alpha 1抗体形式提供，也可作为多种共轭形式的抗AMPK alpha 1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。AMPK（5'-AMP激活的蛋白激酶）是一个异源三聚体复合物，包含一个催化α亚基和调节β和γ亚基。它通过关闭消耗ATP的生物合成途径来保护细胞免受导致ATP耗竭的压力。AMPK通过变构调节、促进上游蛋白激酶（称为AMPK激酶）的磷酸化以及抑制去磷酸化的机制被高AMP和低ATP激活。活化的AMPK可以在体内磷酸化和调节羟甲基戊二酰CoA还原酶和乙酰CoA羧化酶，它们分别是固醇合成和脂肪酸合成的关键调节酶。人类AMPKα1和AMPKα2基因分别编码548个氨基酸和552个氨基酸的蛋白质。人类AMPKβ1编码一个271个氨基酸的蛋白质，人类AMPKβ2编码一个272个氨基酸的蛋白质。人类AMPKγ1基因编码一个331个氨基酸的蛋白质。人类AMPKγ2和AMPKγ3分别是569个和492个氨基酸的蛋白质，包含独特的N端结构域，并可能直接参与AMPK复合物内AMP的结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AMPK alpha 1 抗体 (H-4) 参考文献:

  1. AMP 活化蛋白激酶 gamma 亚基同工酶的特征及其在 AMP 结合中的作用。  |  Cheung, PC., et al. 2000. Biochem J. 346 Pt 3: 659-69. PMID: 10698692
  2. 哺乳动物 AMP 激活蛋白激酶亚家族。  |  Stapleton, D., et al. 1996. J Biol Chem. 271: 611-4. PMID: 8557660
  3. AMP-活化蛋白激酶--哺乳动物细胞的燃料调节器？  |  Hardie, DG. and Carling, D. 1997. Eur J Biochem. 246: 259-73. PMID: 9208914
  4. AMP激活的蛋白激酶同工酶家族：亚基结构和染色体位置。  |  Stapleton, D., et al. 1997. FEBS Lett. 409: 452-6. PMID: 9224708
  5. 鉴定在骨骼肌中高表达的新型 AMP 激活蛋白激酶 beta 亚基同工酶。  |  Thornton, C., et al. 1998. J Biol Chem. 273: 12443-50. PMID: 9575201

订购信息

产品名称产品编号规格价格数量收藏夹

AMPK alpha 1 抗体 (H-4)

sc-398861
200 µg/ml
$316.00

AMPK alpha 1 (H-4): m-IgGκ BP-HRP 套装

sc-524509
200 µg Ab, 40 µg BP
$354.00

AMPK alpha 1 (H-4): m-IgG2b BP-HRP 套装

sc-549167
200 µg Ab; 10 µg BP
$354.00

AMPK alpha 1 抗体 (H-4) AC

sc-398861 AC
500 µg/ml, 25% agarose
$416.00

AMPK alpha 1 抗体 (H-4) HRP

sc-398861 HRP
200 µg/ml
$316.00

AMPK alpha 1 抗体 (H-4) FITC

sc-398861 FITC
200 µg/ml
$330.00

AMPK alpha 1 抗体 (H-4) PE

sc-398861 PE
200 µg/ml
$343.00

AMPK alpha 1 抗体 (H-4) Alexa Fluor® 488

sc-398861 AF488
200 µg/ml
$357.00

AMPK alpha 1 抗体 (H-4) Alexa Fluor® 546

sc-398861 AF546
200 µg/ml
$357.00

AMPK alpha 1 抗体 (H-4) Alexa Fluor® 594

sc-398861 AF594
200 µg/ml
$357.00

AMPK alpha 1 抗体 (H-4) Alexa Fluor® 647

sc-398861 AF647
200 µg/ml
$357.00

AMPK alpha 1 抗体 (H-4) Alexa Fluor® 680

sc-398861 AF680
200 µg/ml
$357.00

AMPK alpha 1 抗体 (H-4) Alexa Fluor® 790

sc-398861 AF790
200 µg/ml
$357.00

AMPK alpha 1 (H-4) 中和勝肽

sc-398861 P
100 µg/0.5 ml
$68.00

I don't have any more of my standard secondary antibody. What do you recommend I use as a secondary detection reagent?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 4 out of 5 by from good!Ho ricevuto il campione omaggio un mese fa circa. L'ho testato e funziona bene
Date published: 2021-04-05
Rated 5 out of 5 by from Band is great!At 1:500, this antibody worked for us. The band is great, but may be a little below the anticipated band size. This is probably just because of our gel make up.
Date published: 2018-11-28
Rated 3 out of 5 by from Good workGives a non specific band in the lymphoma cell lines at around 70 and 60 (although faint compared to the specific band)
Date published: 2018-05-16
Rated 3 out of 5 by from It does not work wellIt does not work well in Hela cells and mouse liver samples when I used 1:500 dilution
Date published: 2018-04-03
Rated 5 out of 5 by from Segnale pulitoHo utilizzato il campione di prova a dicembre 2017. Ho diluito l'anticorpo 1:500 in PBS + BSA. Il segnale è molto pulito, poche bande aspecifiche. Lo raccomando!
Date published: 2018-01-18
Rated 4 out of 5 by from WB AMPK α1 (H-4)One strong band at 63 kDa, with a bit of background (two other bands and aspecific bands).
Date published: 2017-10-13
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Great featuresI applied a sample one month ago and so happy that I did Western Blotting (dilution 1:100), the result is satisfied for me, the band is clear, its position is right.
Date published: 2017-09-20
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AMPK alpha 1 抗体 (H-4) is rated 4.5 out of 5 by 13.
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