Date published: 2025-9-6
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AMPK alpha 1/2 Antibody (D-6) - Western Blotting - Image 309651
AMPKα1/2 抗体 (D-6): sc-74461. IB4 (A), MCF7 (B), Jurkat (C), HeLa (D) 和 K-562 (E) 全细胞裂解液中 AMPKα1/2 表达的 Western 印迹分析.
AMPKα1/2 抗体 (D-6): sc-74461. IB4 (A), MCF7 (B), Jurkat (C), HeLa (D) 和 K-562 (E) 全细胞裂解液中 AMPKα1/2 表达的 Western 印迹分析.

AMPK alpha 1/2 抗体 (D-6): sc-74461

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说明书
  • AMPK alpha 1/2 抗体 D-6 是小鼠单克隆 IgG2a κ，AMPK alpha 1/2抗体, 在147篇文献中引用，规格为200 µg/ml
  • 针对氨基酸251-550，在AMPKα1的human起源的C-terminus处进行映射
  • AMPK alpha 1/2 抗体 (D-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 AMPKα1 and AMPKα2; 也和以下物种反应，包括： canine
  • 抗AMPK alpha 1/2抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取AMPK alpha 1/2 (D-6): sc-74461的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 AMPK alpha 1/2 抗体 (D-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与AMPK alpha 1/2 抗体 (D-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加AMPK抗体，包括AMPK α 1, AMPK α 2, AMPK β1, AMPK β2和AMPK γ 2

AMPKα1/2抗体（D-6）是一种小鼠单克隆IgG2a kappa轻链抗体，针对位于人源AMPKα1 C端的氨基酸251-550，对小鼠、大鼠和人类均具有反应性。抗AMPKα1/2抗体（D-6）靶向AMP激活蛋白激酶（AMPK）的α亚基（α1和α2），后者是细胞代谢的重要能量传感器和调节器。AMPK是一种异源三聚体复合物，由催化性α亚基和调节性β和γ亚基组成。当细胞内AMP/ATP比值升高时，AMPK就会激活，表明能量供应不足。一旦激活，AMPK就会磷酸化各种下游靶标，抑制消耗ATP的合成代谢途径（如脂肪酸和胆固醇合成），并刺激产生ATP的分解代谢途径（如葡萄糖摄取和脂肪酸氧化）。这种调节对于维持能量平衡至关重要，对糖尿病、肥胖症和癌症等代谢紊乱也有重大意义。AMPK活性失调与胰岛素抵抗和肿瘤生长有关，因此AMPK成为治疗研究的重要焦点。AMPKα1/2 (D-6)抗体适用于蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、酶联免疫吸附测定（ELISA）和免疫组织化学等应用，为研究细胞能量调节、代谢途径和相关疾病的研究人员提供了宝贵的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AMPK alpha 1/2 抗体 (D-6) 参考文献:

  1. AMPKα1-LDHA是一种新的代谢途径，可调节干细胞命运。  |  Theret, M., et al. 2018. Cell Cycle. 17: 403-404. PMID: 29316838
  2. AMPK在调节骨骼肌大小、肥大及再生中的作用。  |  Thomson, DM. 2018. Int J Mol Sci. 19: PMID: 30314396
  3. 内皮 AMPKα1/PRKAA1 会加剧高饱和脂肪酸喂养小鼠的炎症。  |  Yang, Q., et al. 2022. Br J Pharmacol. 179: 1661-1678. PMID: 34796475
  4. Empagliflozin 通过激活 AMPKα1/ULK1/FUNDC1/mitophagy 通路减轻心脏微血管缺血/再灌注。  |  Cai, C., et al. 2022. Redox Biol. 52: 102288. PMID: 35325804
  5. AMP 激活的蛋白激酶 α1磷酸化 PHD2，以维持全身铁平衡。  |  Wang, C., et al. 2022. Clin Transl Med. 12: e854. PMID: 35538889
  6. EID3通过抑制AMPKα1促进胶质瘤细胞增殖和存活。  |  Xiang, Y., et al. 2022. J Korean Neurosurg Soc. 65: 790-800. PMID: 36344477
  7. 下丘脑AMPKα1的小细胞外囊泡靶向作用可促进瘦素受体缺陷小鼠的体重减轻。  |  Milbank, E., et al. 2023. Metabolism. 139: 155350. PMID: 36423694
  8. AMPKα1的持续激活通过促进骨源性IGF-1的分泌来防止小鼠骨骼衰老。  |  Yang, Y., et al. 2023. Cell Prolif. 56: e13476. PMID: 37042047
  9. AMPKα1介导的ZDHHC8磷酸化促进SLC7A11的棕榈酰化，从而增强胶质母细胞瘤对铁死亡的耐受性。  |  Wang, Z., et al. 2024. Cancer Lett. 584: 216619. PMID: 38211651
  10. 尼古兰地可减轻糖尿病性心肌病的心脏微血管铁死亡：线粒体定位的AMPK-Parkin-ACSL4信号通路的作用。  |  Chen, Z., et al. 2024. Pharmacol Res. 200: 107057. PMID: 38218357
  11. YAP上调AMPKα1以诱导癌细胞衰老。  |  Zhan, Y., et al. 2024. Int J Biochem Cell Biol. 170: 106559. PMID: 38499237

订购信息

产品名称产品编号规格价格数量收藏夹

AMPK alpha 1/2 抗体 (D-6)

sc-74461
200 µg/ml
RMB2377.00

AMPK alpha 1/2 (D-6): m-IgG Fc BP-HRP 套装

sc-525713
200 µg Ab; 10 µg BP
RMB2662.00

AMPK alpha 1/2 抗体 (D-6) AC

sc-74461 AC
500 µg/ml, 25% agarose
RMB3129.00

AMPK alpha 1/2 抗体 (D-6) HRP

sc-74461 HRP
200 µg/ml
RMB2377.00

AMPK alpha 1/2 抗体 (D-6) FITC

sc-74461 FITC
200 µg/ml
RMB2482.00

AMPK alpha 1/2 抗体 (D-6) PE

sc-74461 PE
200 µg/ml
RMB2580.00

AMPK alpha 1/2 抗体 (D-6) Alexa Fluor® 488

sc-74461 AF488
200 µg/ml
RMB2685.00

AMPK alpha 1/2 抗体 (D-6) Alexa Fluor® 546

sc-74461 AF546
200 µg/ml
RMB2685.00

AMPK alpha 1/2 抗体 (D-6) Alexa Fluor® 594

sc-74461 AF594
200 µg/ml
RMB2685.00

AMPK alpha 1/2 抗体 (D-6) Alexa Fluor® 647

sc-74461 AF647
200 µg/ml
RMB2685.00

AMPK alpha 1/2 抗体 (D-6) Alexa Fluor® 680

sc-74461 AF680
200 µg/ml
RMB2685.00

AMPK alpha 1/2 抗体 (D-6) Alexa Fluor® 790

sc-74461 AF790
200 µg/ml
RMB2685.00

Is it possible to obtain the human phopho-AMPK alpha antibody?

Asked by: omics
Thank you for your question. We do not currently have phopho-AMPK alpha antibody available.
Answered by: Zhanglong
Date published: 2023-11-23

Does this antibody recognize both AMPK1 and AMPK2? Thanks!

Asked by: mito2020
Thanks for your questions.Anti-AMPK alpha 1/2 Antibody (D-6):sc-74461 is recommended for detection of AMPKα1 and AMPKα2 of mouse, rat and human origin.
Answered by: Technical Support 9
Date published: 2020-06-14

When staining cells or tissue, should nuclear or cytoplasmic staining be expected?

Asked by: AbPolly
Thank you for your inquiry. For AMPK alpha 1 and AMPK alpha 2, both nuclear and cytoplasmic localizations have been observed. For your specific sample type, it is best to refer to literature.
Answered by: Technical Support 12
Date published: 2017-01-04
  • y_2025, m_9, d_6, h_5CST
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Rated 5 out of 5 by from GoodGreat antibody for WB, works in mouse RAW 264.7 cells
Date published: 2025-03-18
Rated 5 out of 5 by from Great features for western blotThe antibody can give a nice clean band. It is a good antibody.
Date published: 2024-07-01
Rated 4 out of 5 by from Phosphorylation of LKB1 by PDK1 Inhibits Cell ProlThe master kinase LKB1 is a key regulator of se veral cellular processes, including cell proliferation, cell polarity and cellular metabolism. It phosphorylates and activates several downstream kinases, including AMP-dependent kinase, AMPK. Activation of AMPK by low energy supply and phosphorylation of LKB1 results in an inhibition of mTOR, thus decreasing energy-consuming processes, in particular translation and, thus, cell growth. LKB1 itself is a constitutively active kinase, which is regulated by posttranslational modifications and direct binding to phospholipids of the plasma membrane. Here, we report that LKB1 binds to Phosphoinositide-dependent kinase (PDK1) by a conserved binding motif. Furthermore, a PDK1-consensus motif is located within the kinase domain of LKB1 and LKB1 gets phosphorylated by PDK1 in vitro. In Drosophila, knockin of phosphorylation-deficient LKB1 results in normal survival of the flies, but an increased activation of LKB1, whereas a phospho-mimetic LKB1 variant displays decreased AMPK activation. As a functional consequence, cell growth as well as organism size is decreased in phosphorylation-deficient LKB1. Molecular dynamics simulations of PDK1-mediated LKB1 phosphorylation revealed changes in the ATP binding pocket, suggesting a conformational change upon phosphorylation, which in turn can alter LKB1’s kinase activity. Thus, phosphorylation of LKB1 by PDK1 results in an inhibition of LKB1, decreased activation of AMPK and enhanced cell growth.
Date published: 2023-03-15
Rated 5 out of 5 by from goodthis antibody works well in skeletal muscle of mice.
Date published: 2022-10-01
Rated 4 out of 5 by from Good antibodyMultiple bands were detected but still expected size band was detected
Date published: 2021-12-03
Rated 5 out of 5 by from ottimo funzionamentoSono molto soddisfatto per l'acquisto di questo anticorpo
Date published: 2021-10-04
Rated 5 out of 5 by from OTTIMO FUNZIONAMENTOSONO RIMASTO MOLTO SODDISFATTO DALL'ACQUISTO DI QUESTO ANTICORPO
Date published: 2021-07-07
Rated 5 out of 5 by from 功能出色原来使用过Santa Cruz的抗体，在使用该产品时，WB有良好的条带，使用效果比较好，下次还会购买此产品。
Date published: 2020-11-26
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AMPK alpha 1/2 抗体 (D-6) is rated 4.5 out of 5 by 57.
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