Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
AFP/Alpha fetoprotein Antibody (39) - Western Blotting - Image 352746
AFP 抗体 (39): sc-130302. HeLa (A), A549 (B) 和 Jurkat (C) 全细胞裂解液中 AFP 表达的 Western 印迹分析.
AFP 抗体 (39): sc-130302. HeLa (A), A549 (B) 和 Jurkat (C) 全细胞裂解液中 AFP 表达的 Western 印迹分析.

AFP/Alpha fetoprotein 抗体 (39): sc-130302

4.8(8)
寫評論提問

说明书
  • AFP/Alpha fetoprotein抗体(39)是小鼠单克隆IgG1 κ, 在18篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组AFP
  • 抗-AFP/Alpha fetoprotein 抗体 (39) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 AFP
  • 抗AFP/Alpha fetoprotein抗体（39）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取AFP/Alpha fetoprotein (39): sc-130302的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 AFP/Alpha fetoprotein 抗体 (39) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与AFP/Alpha fetoprotein 抗体 (39) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

AFP/甲胎蛋白抗体（39）是一种IgG1 κ小鼠单克隆AFP抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的AFP。AFP/甲胎蛋白抗体（39）既可以是非偶联的抗AFP抗体形式，也可以是多种偶联形式的抗AFP抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。α-胎蛋白（AFP）在胎儿发育过程中的肝脏中以不同水平表达，在正常成人组织中仅微量存在。在肝细胞癌以及患有肝癌的成人的血浆和腹水中可以检测到异常高浓度的AFP。高浓度的AFP与肿瘤细胞的生长相关，表明AFP可以作为肿瘤标志物。AFP可以结合铜、镍和脂肪酸，并且在某些情况下可以结合血清白蛋白或雌激素。已经证明，AFP启动子是NF-1（核因子-1）、HNF-1（肝细胞核因子-1）和C/EBP转录因子的靶标。虽然HNF-1与AFP启动子的结合导致AFP表达，但NF-1的结合导致AFP启动子活性降低。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

AFP/Alpha fetoprotein 抗体 (39)

sc-130302
200 µg/ml
RMB2377.00

AFP/Alpha fetoprotein (39): m-IgG Fc BP-HRP 套装

sc-525738
200 µg Ab; 10 µg BP
RMB2662.00

AFP/Alpha fetoprotein 抗体 (39) AC

sc-130302 AC
500 µg/ml, 25% agarose
RMB3129.00

AFP/Alpha fetoprotein 抗体 (39) HRP

sc-130302 HRP
200 µg/ml
RMB2377.00

AFP/Alpha fetoprotein 抗体 (39) FITC

sc-130302 FITC
200 µg/ml
RMB2482.00

AFP/Alpha fetoprotein 抗体 (39) PE

sc-130302 PE
200 µg/ml
RMB2580.00

AFP/Alpha fetoprotein 抗体 (39) Alexa Fluor® 488

sc-130302 AF488
200 µg/ml
RMB2685.00

AFP/Alpha fetoprotein 抗体 (39) Alexa Fluor® 546

sc-130302 AF546
200 µg/ml
RMB2685.00

AFP/Alpha fetoprotein 抗体 (39) Alexa Fluor® 594

sc-130302 AF594
200 µg/ml
RMB2685.00

AFP/Alpha fetoprotein 抗体 (39) Alexa Fluor® 647

sc-130302 AF647
200 µg/ml
RMB2685.00

AFP/Alpha fetoprotein 抗体 (39) Alexa Fluor® 680

sc-130302 AF680
200 µg/ml
RMB2685.00

AFP/Alpha fetoprotein 抗体 (39) Alexa Fluor® 790

sc-130302 AF790
200 µg/ml
RMB2685.00

I am using AFP (39): sc-130302 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_130302, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from IHC(paraffin) mouse liverEDTA antigen retrieval, 1:200 dilution works great on mouse liver tumor tissue.
Date published: 2018-01-15
Rated 5 out of 5 by from IHC(paraffin)EDTA(pH 8.0) and heat (95C, 20 min) works fine on paraffin embedded liver tumor tissue.
Date published: 2017-12-25
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of AFP expression in HeLa, A549 and Jurkat whole cell lysates.
Date published: 2017-02-01
Rated 5 out of 5 by from WB Published with this antibody using ratWB Published with this antibody using rat astroglial cells for immunoblot analysis -SCBT Publication Review
Date published: 2015-05-17
Rated 5 out of 5 by from Publishable Western blot results using baboonPublishable Western blot results using baboon hepatocytes. -SCBT Publication Review
Date published: 2015-05-04
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-02-02
Rated 4 out of 5 by from Favorable band in WB using human AFP transfectedFavorable band in WB using human AFP transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-01-06
Rated 4 out of 5 by from Very large robust signal with some backgroundVery large robust signal with some background obtained using Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-01-03
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_130302, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
AFP/Alpha fetoprotein 抗体 (39) is rated 4.8 out of 5 by 8.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_130302, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 111ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。