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AFP/Alpha fetoprotein Antibody (C3) - Western Blotting - Image 352745
AFP 抗体 (C3): sc-8399. 人胎儿肝组织提取物中 AFP 表达的 Western 印迹分析.
AFP 抗体 (C3): sc-8399. 人胎儿肝组织提取物中 AFP 表达的 Western 印迹分析.

AFP/Alpha fetoprotein 抗体 (C3): sc-8399

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说明书
  • AFP/Alpha fetoprotein 抗体 C3 是小鼠单克隆 IgG2a κ，AFP/Alpha fetoprotein抗体, 在88篇文献中引用，规格为200 µg/ml
  • 免疫1-609氨基酸，对应于human来源AFP全长
  • AFP/Alpha fetoprotein 抗体 (C3) 推荐用于 WB, IP, IF, FCM 和 ELISA，检测mouse, rat 和human 来源的 AFP
  • 抗AFP/Alpha fetoprotein抗体（C3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取AFP/Alpha fetoprotein (C3): sc-8399的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是AFP/Alpha fetoprotein Antibody (C3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与AFP/Alpha fetoprotein Antibody (C3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

AFP抗体（C3）是一种小鼠单克隆IgG2a kappa轻链抗体，用于检测多种物种（包括小鼠、大鼠和人类）中的甲胎蛋白（AFP），也称为甲型1胎蛋白或甲胎蛋白。抗AFP抗体（C3）可识别全长AFP蛋白，识别氨基酸1-609，在多种应用中均有效，如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）。AFP在胎儿发育过程中起着至关重要的作用，主要在肝脏中表达，在成人组织中通常含量极低。AFP的重要性在于其在肝细胞癌和其他与肝脏相关的癌症中的水平升高，这使得AFP成为早期检测和监测癌症进展的重要肿瘤标志物。此外，AFP能够与铜和镍等必需金属以及脂肪酸结合，并且可能与血清白蛋白或雌激素相互作用，这凸显了其多功能性。AFP表达的调节涉及关键转录因子，如NF-1、HNF-1和C/EBP，其中HNF-1促进AFP表达，NF-1抑制其启动子活性。AFP单克隆抗体（C3）有非结合型和多种结合型，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®变体，可满足各种实验需求。抗AFP抗体（C3）是研究肝发育、癌症生物学和涉及AFP的蛋白质相互作用的研究人员必不可少的工具，可确保研究结果准确可靠。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AFP/Alpha fetoprotein 抗体 (C3) 参考文献:

  1. 甲胎蛋白和白蛋白对大鼠肝癌细胞和胎鼠肝细胞摄取多不饱和脂肪酸的影响  |  Iturralde, M., et al. 1991. Biochim Biophys Acta. 1086: 81-8. PMID: 1720022
  2. 针对人类甲胎蛋白的单克隆抗体。利用与抗原之间不寻常的钙依赖性相互作用进行分析和制备。  |  Stefanová, I., et al. 1988. J Immunol Methods. 111: 67-73. PMID: 2455753
  3. CAAT/增强子结合蛋白, 肝细胞核因子-1和核因子-1家族成员可对大鼠甲胎蛋白启动子和增强子的活性进行不同程度的调节。  |  Bois-Joyeux, B. and Danan, JL. 1994. Biochem J. 301 (Pt 1): 49-55. PMID: 7518671
  4. 在人类甲胎蛋白基因启动子的控制下，利用携带单纯疱疹病毒胸苷激酶基因的逆转录病毒载体对肝癌细胞进行基因治疗。  |  Ido, A., et al. 1995. Cancer Res. 55: 3105-9. PMID: 7541712
  5. [多种转录因子参与甲胎蛋白基因启动子的功能】。］  |  Bois-Joyeux, B., et al. 1995. Bull Cancer. 82: 541-50. PMID: 7549116
  6. 人类甲胎蛋白的铜（II）结合能力  |  Aoyagi, Y., et al. 1978. Cancer Res. 38: 3483-6. PMID: 80265
  7. 甲胎蛋白刺激肿瘤细胞生长  |  Wang, XW. and Xu, B. 1998. Int J Cancer. 75: 596-9. PMID: 9466662
  8. 黄酮类化合物可抑制雌激素与大鼠甲胎蛋白的结合。  |  Baker, ME., et al. 1998. Proc Soc Exp Biol Med. 217: 317-21. PMID: 9492341

订购信息

产品名称产品编号规格价格数量收藏夹

AFP/Alpha fetoprotein 抗体 (C3)

sc-8399
200 µg/ml
RMB2377.00

AFP/Alpha fetoprotein (C3): m-IgG Fc BP-HRP 套装

sc-525452
200 µg Ab; 10 µg BP
RMB2662.00

AFP/Alpha fetoprotein (C3): m-IgG2a BP-HRP 套装

sc-546264
200 µg Ab; 10 µg BP
RMB2662.00

AFP/Alpha fetoprotein 抗体 (C3) AC

sc-8399 AC
500 µg/ml, 25% agarose
RMB3129.00

AFP/Alpha fetoprotein 抗体 (C3) HRP

sc-8399 HRP
200 µg/ml
RMB2377.00

AFP/Alpha fetoprotein 抗体 (C3) FITC

sc-8399 FITC
200 µg/ml
RMB2482.00

AFP/Alpha fetoprotein 抗体 (C3) PE

sc-8399 PE
200 µg/ml
RMB2580.00

AFP/Alpha fetoprotein 抗体 (C3) Alexa Fluor® 488

sc-8399 AF488
200 µg/ml
RMB2685.00

AFP/Alpha fetoprotein 抗体 (C3) Alexa Fluor® 546

sc-8399 AF546
200 µg/ml
RMB2685.00

AFP/Alpha fetoprotein 抗体 (C3) Alexa Fluor® 594

sc-8399 AF594
200 µg/ml
RMB2685.00

AFP/Alpha fetoprotein 抗体 (C3) Alexa Fluor® 647

sc-8399 AF647
200 µg/ml
RMB2685.00

AFP/Alpha fetoprotein 抗体 (C3) Alexa Fluor® 680

sc-8399 AF680
200 µg/ml
RMB2685.00

AFP/Alpha fetoprotein 抗体 (C3) Alexa Fluor® 790

sc-8399 AF790
200 µg/ml
RMB2685.00

Dear Sir. I already have AFP Antibody (C3): sc-8399. now I want to stain IF in induce hepatocyte. please guide me what product I must buy more to do that purpose. thank you very much!

Asked by: Vanhanhnguyen
Thank you for your question. This antibody is recommended for IF in mouse, rat and human samples. There are many product citations using this antibody for IF staining. We have not specifically tested this antibody in induced hepatocytes, but if AFP is expressed in those samples, then this antibody should be suitable for detection.
Answered by: Technical Support
Date published: 2020-05-07

Can this antibody be used for staining on mouse tissue?

Asked by: Anna99
Yes, by using a directly conjugated primary, you can stain on mouse tissue. This AFP antibody is available conjugated to HRP (sc-8399 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-8399 PE), fluorescein (sc-8399 FITC), Alexa Fluor® 488 (sc-8399 AF488) or Alexa Fluor® 647 (sc-8399 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Support 7
Date published: 2017-01-12

What is your suggested secondary for this mouse monoclonal antibody for use in WB?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-12
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Rated 5 out of 5 by from Segnale buono e specificoAnticorpo testato su linee cellulari HepG2, HeLa e DU145 in immunofluorescenza. Come atteso, le HepG2 sono positive e le altre due linee negative. Segnale buono, no background.
Date published: 2024-04-22
Rated 5 out of 5 by from Great antibody.I got this sample antibody one month ago. It works very well!
Date published: 2019-04-23
Rated 4 out of 5 by from Work well.The antibody work in IF at 1:100 for mouse primary liver cancer cells and hepa1-6 cells, but the FITC isn't especially positive through stained.Maybe the reasons cause by the low concentration of it. Other colleagues should try to increase the concentration if applying IF assay for hepatocarcinoma.(PS: It is known to all that hepa1-6 can express the AFP protein form ATCC)
Date published: 2017-10-24
Rated 4 out of 5 by from Works fine in WBThis AFP antibody works fine in WB with HepG2 cells.
Date published: 2017-10-12
Rated 5 out of 5 by from Best AFP antibodyJust a great product for multiple applications !!!
Date published: 2017-01-18
Rated 5 out of 5 by from Great antibodyThis antibody showed good results for IF staining.
Date published: 2017-01-11
Rated 1 out of 5 by from Not so wellI used it in IF at a dilution of 1:100,didn't see any positive signal.
Date published: 2016-12-22
Rated 5 out of 5 by from Publishable Western blot results using hepatomaPublishable Western blot results using hepatoma cells. -SCBT Publication Review
Date published: 2015-05-29
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AFP/Alpha fetoprotein 抗体 (C3) is rated 4.5 out of 5 by 14.
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