Date published: 2025-9-7
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YAP Antibody (H-9) - Western Blotting - Image 380826
YAP1 抗体 (H-9): sc-271134. Hep G2 (A), U-2 OS (B), NIH/3T3 (C), C6 (D) 和 AT3B-1 (E) 全细胞裂解液中 YAP 表达的 Western 印迹分析.
YAP1 抗体 (H-9): sc-271134. Hep G2 (A), U-2 OS (B), NIH/3T3 (C), C6 (D) 和 AT3B-1 (E) 全细胞裂解液中 YAP 表达的 Western 印迹分析.

YAP 抗体 (H-9): sc-271134

4.7(19)
寫評論提問

说明书
  • YAP抗体(H-9)是小鼠单克隆IgG1 κ, 在70篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的YAP的C-terminus附近的氨基酸379-407之间
  • 抗-YAP 抗体 (H-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 YAP
  • YAP抗体（H-9）可与Alexa Fluor® 546或Alexa Fluor® 594结合，用于WB（RGB）、IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-271134 X, 200 µg/0.1 ml)
  • 关于如何获取YAP (H-9): sc-271134的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 YAP 抗体 (H-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与YAP 抗体 (H-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

YAP1抗体（H-9）是一种IgG1 κ小鼠单克隆YAP抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的YAP。YAP1抗体（H-9）以偶联和非偶联的抗YAP抗体形式提供。Yes相关蛋白，也称为YAP，是一种14-3-3结合分子，最初因其能与Yes的SH3结构域结合而被识别。YAP与14-3-3的结合需要在YAP 14-3-3结合基序中的同源丝氨酸残基（Ser 112）进行磷酸化。高度保守且普遍表达的14-3-3蛋白通过结合参与信号转导的细胞内磷蛋白来调节分化、细胞周期进程和凋亡。YAP可能以由14-3-3蛋白调节的方式将质膜和细胞骨架的事件与细胞核中的转录抑制联系起来。YAP与具有PDZ结合基序的转录共激活因子TAZ的WW结构域具有同源性，其通过结合存在于转录因子中的PPXY基序而作为转录共激活因子发挥作用。YAP在肺、胎盘、前列腺、卵巢和睾丸中高表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

YAP 抗体 (H-9)

sc-271134
200 µg/ml
RMB2377.00

YAP (H-9): m-IgG Fc BP-HRP 套装

sc-525787
200 µg Ab; 10 µg BP
RMB2662.00

YAP 抗体 (H-9) X

sc-271134 X
200 µg/0.1 ml
RMB2377.00

YAP 抗体 (H-9) Alexa Fluor® 546

sc-271134 AF546
200 µg/ml
RMB2685.00

YAP 抗体 (H-9) Alexa Fluor® 594

sc-271134 AF594
200 µg/ml
RMB2685.00

YAP 抗体 (H-9) Alexa Fluor® 680

sc-271134 AF680
200 µg/ml
RMB2685.00

YAP 抗体 (H-9) Alexa Fluor® 790

sc-271134 AF790
200 µg/ml
RMB2685.00

YAP (H-9) 中和勝肽

sc-271134 P
100 µg/0.5 ml
RMB511.00

why using this antibody staining YAP in MCF-7 is located in cytoplasm

Asked by: likeminem
Thank you for your question. Many proteins localize to more than one location in a cell, so localization often varies depending on the cells. YAP has been shown to translocate to the cytoplasm due to phosphorylation and cell density.
Answered by: Tech Service
Date published: 2019-11-06

which yap antibody from your company that can detect phosphorylated yap?

Asked by: siti
Thank you for your question. We do not have a phospo-YAP antibody that specifically detects only phosphorylated YAP. All the antibodies are capable of detecting phosphorylated YAP as well as non-phosphorylated YAP, depending on the epitope and the phosphorylation site.
Answered by: Tech Service
Date published: 2019-09-12

您好，(sc-271134) YAP Antibody (H-9) 免疫染色的示意图显示在HELA细胞的细胞质，另一支抗体(sc-101199) YAP antibody (63.7) 免疫染色示意图显示，YAP在HELA细胞的细胞核，请问这两只抗体有什么差别？

Asked by: Anonymous
感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。 Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 9
Date published: 2019-05-05

Dear company, Could you please help me to consult the price and inventory of the product YAP Antibody (H-9): sc-271134.Many thanks. Yours, Grace

Asked by: Grace Li
Thank you for your question. Please contact one of our authorized distributors for information about current product availability and shipping lead times.
Answered by: Technical Support
Date published: 2019-04-13

What secondary reagent should I use for IHC if I am working with mice tissue samples?

Asked by: Trav11
For cases like this, we recommend using our mouse monoclonal primary antibodies that are directly conjugated. The YAP Antibody (H-9): sc-271134 is NOT currently available directly conjugated, but we do offer an alternative, YAP Antibody (G-6): sc-376830, that is directly conjugated. Currently, sc-376830 is available conjugated to HRP (sc-376830 HRP), phycoerythrin (sc-376830 PE), fluorescein (sc-376830 FITC), Alexa Fluor® 488 (sc-376830 AF488) or Alexa Fluor® 647 (sc-376830 AF647).
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 5 out of 5 by from mouse anti-YAP works well for ICCAnti-YAP works well for ICC on murine brown adipocytes.
Date published: 2023-09-22
Rated 5 out of 5 by from Great antibody!It works well in immunofluorescence. 1:100 dilution
Date published: 2021-10-21
Rated 3 out of 5 by from Bright cytosolic staining, limited nuclear staininMDCKs fixed in 4% paraformaldehyde, stained with primary at 1:100 3 hr at 37C. Secondary goat anti-mouse (Dylight 488) overnight at 4C
Date published: 2018-11-27
Rated 4 out of 5 by from The fluorescence is not so nice in Western Blot.This antibody worked in the Western blot, but the fluorescence is not so nice. It seems to recognize only the cytosolic YAP.
Date published: 2018-05-17
Rated 5 out of 5 by from Good qualityI used this antibody for IHC and the result is good.
Date published: 2018-04-25
Rated 5 out of 5 by from Great featuresI used this antibody on nuclear extraction of pancreatic cancer cell line and have good bands.
Date published: 2018-03-14
Rated 3 out of 5 by from YAP 抗体 (H-9) X ChIP实验我们用这个抗体做ChIP实验，但是没有得到阳性结果，有可能该蛋白并不结合我们的目标区域，也可能是抗体问题，目前无法确认。
Date published: 2017-12-11
Rated 5 out of 5 by from Clean band at expected molecular weight (~65kDa)Sample: MDA-MB-231 wcl (30ug) Blocking: 1h in blocking buffer (0.05% tween-20 in TBS with 5% skin milk). Primary antibody: 1:200 in blocking buffer @ 4oC, overnight. Secondary antibody: 1:3000 in blocking buffer. Exposure time: 30 seconds.
Date published: 2017-11-18
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YAP抗体 (H-9) is rated 4.7 out of 5 by 19.
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