Date published: 2025-9-7
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XBP1 Antibody (F-4) - Western Blotting - Image 393455
XBP-1 抗体 (F-4) Alexa Fluor® 647: sc-8015 AF647. 小鼠重组 XBP-1 融合蛋白的 Western 印迹分析.
XBP1 Antibody (F-4) - Western Blotting - Image 393455
XBP-1 抗体 (F-4) Alexa Fluor® 647: sc-8015 AF647. 小鼠重组 XBP-1 融合蛋白的 Western 印迹分析.
XBP1 Antibody (F-4) - Western Blotting - Image 1258
XBP-1 抗体 (F-4): sc-8015. His 标记小鼠重组 XBP-1 蛋白的 Western 印迹分析.
XBP-1 抗体 (F-4) Alexa Fluor® 647: sc-8015 AF647. 小鼠重组 XBP-1 融合蛋白的 Western 印迹分析.

XBP1 抗体 (F-4): sc-8015

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寫評論提問

说明书
  • XBP1 抗体 F-4 是小鼠单克隆 IgG1 κ，XBP1抗体, 在62篇文献中引用，规格为200 µg/ml
  • 免疫mouse来源XBP-1位于C-terminus 的76-263氨基酸
  • XBP1 抗体 (F-4) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 XBP-1
  • 抗XBP1抗体（F-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-8015 X, 200 µg/0.1 ml)
  • 关于如何获取XBP1 (F-4): sc-8015的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是XBP1 Antibody (F-4) for WB applications. 的首选辅助检测试剂，这些试剂现与XBP1 Antibody (F-4) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

XBP抗体

XBP-1抗体（F-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类的XBP-1。XBP-1抗体（F-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。XBP-1，在小鼠和人类中也被称为税反应元件结合蛋白5（TREB5），在大鼠中被称为肝细胞癌相关转录因子（HTF），是基本区/亮氨酸拉链（bZIP）转录因子家族的重要成员。XBP-1在细胞应激反应中发挥着至关重要的作用，特别是在未折叠蛋白反应（UPR）中，该反应对于在应激条件下维持细胞稳态至关重要。XBP-1主要位于细胞核中，在那里XBP-1调节与蛋白质折叠和降解相关的基因的表达，从而确保正常的细胞功能和存活。XBP-1在发育中的骨骼中的表达特别高，表明它参与了成骨细胞的分化和功能。XBP-1已被证明可与多种蛋白质相互作用，包括其他转录因子和UPR的组成部分，这凸显了其在细胞信号通路中的重要性。XBP-1与cAMP反应元件（CRE）和TPA反应元件（TREs）的结合进一步强调了其在激活关键基因转录中的作用，这使得XBP-1成为癌症和代谢性疾病等领域研究的重要目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

XBP1 抗体 (F-4) 参考文献:

  1. XBP-1 在未折叠蛋白反应中调控内质网常驻伴侣基因子集。  |  Lee, AH., et al. 2003. Mol Cell Biol. 23: 7448-59. PMID: 14559994
  2. 原发性乳腺癌中人类 X-box 结合蛋白 1（hXBP-1）基因的上调和过表达。  |  Fujimoto, T., et al. 2003. Breast Cancer. 10: 301-6. PMID: 14634507
  3. XBP-1 通过调控大规模染色质的展开来提高 ERalpha 的转录活性。  |  Fang, Y., et al. 2004. Biochem Biophys Res Commun. 323: 269-74. PMID: 15351732
  4. 多发性骨髓瘤反应性 CTL 靶向正调控域 I 结合因子 1 和 X 盒结合蛋白 1 转录因子  |  Lotz, C., et al. 2005. J Immunol. 175: 1301-9. PMID: 16002735
  5. 一种 HLA-DR α 启动子 DNA 结合蛋白广泛表达，并与人类的 22 号和 5 号染色体相连。  |  Liou, HC., et al. 1991. Immunogenetics. 34: 286-92. PMID: 1718857
  6. 人类 X-box 结合蛋白 1 是人类 II 类主要组织相容性基因转录所必需的，并与 c-fos 形成异源二聚体。  |  Ono, SJ., et al. 1991. Proc Natl Acad Sci U S A. 88: 4309-12. PMID: 1903538
  7. 与 HLA DR alpha 启动子结合的亮氨酸拉链类蛋白质的新成员。  |  Liou, HC., et al. 1990. Science. 247: 1581-4. PMID: 2321018
  8. IRE1α/XBP-1在囊性纤维化呼吸道炎症中的作用。  |  Ribeiro, CM. and Lubamba, BA. 2017. Int J Mol Sci. 18: PMID: 28075361
  9. 下调的XBP-1通过NLRP3/Caspase-1/GSDMD轴线修复脑缺血/再灌注损伤引起的热解作用。  |  Zhang, Y., et al. 2022. Mediators Inflamm. 2022: 8007078. PMID: 35497095
  10. 鉴定与 HTLV-1 增强子结合的 CREB/ATF 家族蛋白 TREB5 的转录激活结构域。  |  Matsuzaki, Y., et al. 1995. J Biochem. 117: 303-8. PMID: 7608116
  11. 原位杂交研究表明，基本区亮氨酸拉链蛋白 hXBP-1 在小鼠胚胎发育过程中的外分泌腺和骨骼发育中发挥作用。  |  Clauss, IM., et al. 1993. Dev Dyn. 197: 146-56. PMID: 7693055
  12. 碱性结构域/亮氨酸拉链蛋白 hXBP-1 优先结合并转录激活含有 ACGT 核心的 CRE 样序列。  |  Clauss, IM., et al. 1996. Nucleic Acids Res. 24: 1855-64. PMID: 8657566

订购信息

产品名称产品编号规格价格数量收藏夹

XBP1 抗体 (F-4)

sc-8015
200 µg/ml
RMB2377.00

XBP1 (F-4): m-IgG Fc BP-HRP 套装

sc-526506
200 µg Ab; 10 µg BP
RMB2662.00

XBP1 (F-4): m-IgG1 BP-HRP 套装

sc-531879
200 µg Ab; 20 µg BP
RMB2662.00

XBP1 抗体 (F-4) X

sc-8015 X
200 µg/0.1 ml
RMB2377.00

XBP1 抗体 (F-4) AC

sc-8015 AC
500 µg/ml, 25% agarose
RMB3129.00

XBP1 抗体 (F-4) HRP

sc-8015 HRP
200 µg/ml
RMB2377.00

XBP1 抗体 (F-4) FITC

sc-8015 FITC
200 µg/ml
RMB2482.00

XBP1 抗体 (F-4) PE

sc-8015 PE
200 µg/ml
RMB2580.00

XBP1 抗体 (F-4) Alexa Fluor® 488

sc-8015 AF488
200 µg/ml
RMB2685.00

XBP1 抗体 (F-4) Alexa Fluor® 546

sc-8015 AF546
200 µg/ml
RMB2685.00

XBP1 抗体 (F-4) Alexa Fluor® 594

sc-8015 AF594
200 µg/ml
RMB2685.00

XBP1 抗体 (F-4) Alexa Fluor® 647

sc-8015 AF647
200 µg/ml
RMB2685.00

XBP1 抗体 (F-4) Alexa Fluor® 680

sc-8015 AF680
200 µg/ml
RMB2685.00

XBP1 抗体 (F-4) Alexa Fluor® 790

sc-8015 AF790
200 µg/ml
RMB2685.00

Hi there, I am hoping to find out if this antibody detects both XBP1u and XBP1s, or only XBP1s? Thanks in advance.

Asked by: xkon7228
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-10-09

Dear Customer Service, I got interested in this antibody because it was referenced in a Caenorhabditis elegans-specific database. Is there any data supporting a crossreaction with the C. elegans XBP-1 protein?

Asked by: c elegans
Thank you for your question. Anti-XBP-1 Antibody (F-4) is only recommended for detection of XBP-1 of mouse, rat and human origin by WB, IP, IF and ELISA. We have not tested it with C. elegans samples and cannot say whether the antibody detect its target protein of that origin, unfortunately. There is very little homology between the sequence of C. elegans protein and mouse protein, so cross-reactivity is very unlikely.
Answered by: Tech Support Europe
Date published: 2021-11-11

Hi, I am looking for XBP1 antibody for Chip assay with mouse cells. XBP1 antibody (M186) was discontinued. Do you have any data demonstrating XBP-1 Antibody (F-4) sc-8015 works well for Chip assay with mouse cells? Any guarantee?Thank you.

Asked by: Toru
Thank you for your question. This antibody has not yet been tested in a ChIP application, and there are no references that we are aware of at this time for ChIP. It is, however, warrantied for ChIP use.
Answered by: Tech Service
Date published: 2019-06-10

Will this recognize human XBP-1?

Asked by: jmb1
Thank you for your question. The sc-8015 is for detection of XBP-1 of human, mouse and rat origin.
Answered by: Technical Support
Date published: 2019-02-10

When should the TransCruz reagent sc-8015 X be used instead of the standard form sc-8015?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 9
Date published: 2017-01-17
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Rated 1 out of 5 by from Non funziona per IHCI was interested in immunohistochemical revelation of this protein, but unfortunately (as stated in the datasheet), I can’t achive a signal on FFPE paraffined section of reactive tonsil (physical antigen retrieval CITRATE pH6.0 or EDTA pH8.0 using microwave/water bath or chimical antigen retrival using protease)
Date published: 2021-06-14
Rated 4 out of 5 by from Works as portrayedThe antibody showed a band at 55 Kda and also a small band at 43. Had to incubate the primary at room temperature overnight to get a band
Date published: 2018-10-09
Rated 3 out of 5 by from It is not so goodI didn't find the band which I was want to, and the background is not clear.
Date published: 2018-02-14
Rated 3 out of 5 by from goodThis XBP-1 antibody works for western but has very strong nonspecific bands
Date published: 2018-02-02
Rated 2 out of 5 by from Unspecific bands on mouse heart lysatesUnfortunately I obtained many unspecific bands on mouse heart lysates, therefor it is difficult to determine the right bands.
Date published: 2017-11-23
Rated 1 out of 5 by from Skeletal muscle immunostainingThis antibody did not work for the immunostaining of frozen rat skeletal muscle sections.
Date published: 2017-11-22
Rated 3 out of 5 by from the band is not very clearI used this antibody with a dilution of 1:200. However, the band was not very clear.
Date published: 2017-10-23
Rated 5 out of 5 by from 功能不错我一个月前申请了改抗体的试用装，很高兴能提供试用反馈，这个抗体还不错，洗脱过的膜上也杂处条带了,虽然不是很清晰，但是没有杂带，特异性不错。
Date published: 2017-09-27
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XBP1 抗体 (F-4) is rated 3.8 out of 5 by 27.
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