Date published: 2025-9-7
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LEF-1 Antibody (2D12) - Western Blotting - Image 37138
WT1 (F-6): sc-7385. 非转染: sc-117752 (A) 和截短的人 WT1 转染: sc-117178 (B) 293T 全细胞裂解液中 WT1 表达的 Western 印迹分析.
LEF-1 Antibody (2D12) - Western Blotting - Image 37138
WT1 (F-6): sc-7385. 非转染: sc-117752 (A) 和截短的人 WT1 转染: sc-117178 (B) 293T 全细胞裂解液中 WT1 表达的 Western 印迹分析.
WT1 Antibody (F-6) - Western Blotting - Image 870
WT1 抗体 (F-6): sc-7385. 对 K-562 全细胞裂解液中 WT1 的表达进行 Western 印迹分析.
WT1 (F-6): sc-7385. 非转染: sc-117752 (A) 和截短的人 WT1 转染: sc-117178 (B) 293T 全细胞裂解液中 WT1 表达的 Western 印迹分析.

WT1 抗体 (F-6): sc-7385

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寫評論提問

说明书
  • WT1 抗体 F-6 是小鼠单克隆 IgG1，WT1抗体, 在154篇文献中引用，规格为200 µg/ml
  • 与WT1 of human来源的N-terminus附近长度为180个氨基酸的域对应的表位
  • WT1 抗体 (F-6) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 WT1
  • WT1抗体（F-6）可与琼脂糖结合用于IP
  • WT1抗体（F-6）可与Alexa Fluor® 546或Alexa Fluor® 594结合，用于WB（RGB）、IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-7385 X, 200 µg/0.1 ml)
  • 关于如何获取WT1 (F-6): sc-7385的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是WT1 Antibody (F-6) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与WT1 Antibody (F-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

PKC ζ 抗体（H-1）是一种小鼠单克隆IgG2a抗体，靶向大鼠源蛋白激酶C zeta（PKC ζ）C末端573-592位氨基酸之间的表位，也称为PRKCZ或nPKC-ζ。PKC ζ 单克隆抗体（H-1）可与小鼠、大鼠和人类反应，适用于包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）在内的多种应用。PKC ζ是蛋白激酶C家族中一个非典型的成员，在细胞生长、分化、存活、基因表达和膜功能等各种细胞过程中发挥着关键作用。与常规的PKC不同，PKC ζ的激活与钙和二酰基甘油（DAG）无关，在信号传导通路中具有独特的功能。PKC ζ参与NF-κB激活、胰岛素信号传导、细胞极性和PI3K/Akt通路的调节，因此对于理解糖尿病、癌症和免疫失调等疾病具有重要意义。PKC ζ参与细胞存活和增殖，因此作为潜在的治疗靶点非常重要。此外，独特的激活机制使PKC ζ成为信号转导研究中一个引人入胜的课题，有可能揭示细胞通讯通路的新见解。PKC ζ 单克隆抗体（H-1）使研究人员能够有效地研究其在各种生物学环境中的表达和功能，从而有助于开发新的治疗方法。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

WT1 抗体 (F-6) 参考文献:

  1. Wilms 肿瘤患者 WT1 基因中的锌指点突变。  |  Little, MH., et al. 1992. Proc Natl Acad Sci U S A. 89: 4791-5. PMID: 1317572
  2. Wilms 肿瘤抑制因子 WT1 对胰岛素样生长因子 II 基因的抑制。  |  Drummond, IA., et al. 1992. Science. 257: 674-8. PMID: 1323141
  3. Wilms 肿瘤基因产物 WT1 可抑制血小板衍生生长因子 A 链基因的转录。  |  Wang, ZY., et al. 1992. J Biol Chem. 267: 21999-2002. PMID: 1429549
  4. WT1 Wilms 肿瘤基因座编码的锌指蛋白的特征。  |  Morris, JF., et al. 1991. Oncogene. 6: 2339-48. PMID: 1662794
  5. 肿瘤抑制因子和癌基因：WT1 的故事。  |  Yang, L., et al. 2007. Leukemia. 21: 868-76. PMID: 17361230
  6. 人类第 11 号染色体 Wilms 肿瘤基因座上的锌指多肽基因的分离与鉴定。  |  Call, KM., et al. 1990. Cell. 60: 509-20. PMID: 2154335
  7. 通过染色体跳跃鉴定出 Wilms 肿瘤中锌指基因的同基因缺失。  |  Gessler, M., et al. 1990. Nature. 343: 774-8. PMID: 2154702
  8. 将正常人的 11 号染色体导入 Wilms 肿瘤细胞系可控制其致癌表达。  |  Weissman, BE., et al. 1987. Science. 236: 175-80. PMID: 3031816
  9. Wilms 肿瘤的遗传学。  |  Matsunaga, E. 1981. Hum Genet. 57: 231-46. PMID: 6265341

订购信息

产品名称产品编号规格价格数量收藏夹

WT1 抗体 (F-6)

sc-7385
200 µg/ml
RMB2377.00

WT1 (F-6): m-IgG1 BP-HRP 套装

sc-551910
200 µg Ab; 20 µg BP
RMB2662.00

WT1 抗体 (F-6) X

sc-7385 X
200 µg/0.1 ml
RMB2377.00

WT1 抗体 (F-6) AC

sc-7385 AC
500 µg/ml, 25% agarose
RMB3129.00

WT1 抗体 (F-6) Alexa Fluor® 546

sc-7385 AF546
200 µg/ml
RMB2685.00

WT1 抗体 (F-6) Alexa Fluor® 594

sc-7385 AF594
200 µg/ml
RMB2685.00

WT1 抗体 (F-6) Alexa Fluor® 680

sc-7385 AF680
200 µg/ml
RMB2685.00

WT1 抗体 (F-6) Alexa Fluor® 790

sc-7385 AF790
200 µg/ml
RMB2685.00

Is this antibody suitable for IHC-Fr?

Asked by: zju318
Thanks for your questions. Anti-WT1 Antibody (F-6) is recommended for detection of WT1 of mouse, rat and human origin by IF on frozen tissue. 感谢您的提问。 Anti-WT1 Antibody (F-6) 推荐检检测人源，大鼠和小鼠来源的冰冻切片的 WT1。
Answered by: Yanru Tao
Date published: 2022-09-05

Hello, I purchased this product (WT1 Antibody (F-6): sc-7385 with Alex FLour 680). I found that it can be used for Flow, but i couldn't find any publications for that. Can you please send me any documents that shows it works in using flow cytometry?

Asked by: Kiarash
Thank you for your question, and for sharing your feedback with us! We are currently aware of one product citation using this monoclonal antibody for Flow Cytometry. Please refer to PMID: 19092120 for details. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2020-05-08

请问WT1（F-6）是不是识别N端 1-180aa抗原？

Asked by: qxw9908
感谢您的提问。这边只说明是靠近N端，关于更详细的抗原信息，欢迎通过：电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-01-02

Is this antibody appropriate for use in IHC with paraffin-embedded sections?

Asked by: AbPolly
Thank you for your question. While this antibody has not been validated for use with paraffin-embedded samples by our labs, there are publications siting this antibody for use in IHC(P). Please contact our Technical Service Department for further details and recommendations.
Answered by: Technical Support 12
Date published: 2017-01-04
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Rated 1 out of 5 by from Did not work for WBDid not work well for WB of neonatal mouse lung proteins.
Date published: 2024-12-13
Rated 1 out of 5 by from 免疫組織化学染色でうまく機能しない精巣のパラフィン切片を使って免疫蛍光染色を行いましたが、 セルトリ細胞の核が全く染色されませんでした。
Date published: 2020-12-23
Rated 2 out of 5 by from WB on mouse kidneyTested on Kidney extract (40ug) I have observed 3 different bands in WB, no one really corresponding to the expected band (52kDa).
Date published: 2020-08-10
Rated 5 out of 5 by from Dr SadekAb showed perfect expression with 1:200 dilution and 2 hrs incubation at 37℃ and overnight at 4℃
Date published: 2018-11-21
Rated 5 out of 5 by from Grreat resultsBought this many times already. Great product. Would buy again and again.
Date published: 2018-04-22
Rated 5 out of 5 by from clear bandWe use this antibody with WT1 in 1:1000 dilution is fit for western.
Date published: 2017-08-16
Rated 5 out of 5 by from A wonderful antibody!I bought this antibody two weeks ago for the western blot and immunofluorescence on the mouse cells and tissues, it works so great. It is definitly an ideal antibody for the WT1 study.
Date published: 2017-06-11
Rated 5 out of 5 by from very nice resultWorks very nicely for IF on mouse samples. We published with it and will use again!
Date published: 2017-05-27
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WT1抗体 (F-6) is rated 4.3 out of 5 by 20.
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