Date published: 2025-9-7

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WT1 抗体 (H-1): sc-393498

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说明书
  • WT1抗体(H-1)是小鼠单克隆IgM κ, 在23篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的WT1的内部区域内的氨基酸123-164之间
  • 推荐用于 mouse, rat 和 human 来源的WT1 WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应,包括: 和 bovine and porcine
  • 关于如何获取WT1 (H-1): sc-393498免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成WT1 抗体 (H-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

WT1抗体(H-1)是一种IgM κ小鼠单克隆WT1抗体,可通过WB、IP、IF、IHC(P)和ELISA检测到来源于小鼠、大鼠和人类的WT1。WT1抗体(H-1)提供非偶联的抗体形式。Wilms瘤(WT)是一种影响肾脏的胚胎恶性肿瘤,影响比例为万分之一的婴儿,并且像视网膜母细胞瘤一样,既有散发形式也有遗传形式。Wilms瘤基因座已被定位在11p13染色体上,作为一种抑癌基因,编码一种具有四个锌指和一个富含谷氨酰胺-脯氨酸的氨基末端的DNA结合蛋白。Wilms瘤蛋白(WT1)与DNA序列GCGGGGGCG结合,这是早期生长反应(Egr)家族的Zn2+指转录激活因子常见的识别元件。然而,与Egr转录因子相反,WT1在与合成启动子结构的瞬时转染测定中表现为转录抑制因子。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

WT1 抗体 (H-1)

sc-393498
200 µg/ml
RMB2377.00

WT1 (H-1) 中和勝肽

sc-393498 P
100 µg/0.5 ml
RMB511.00

I would like to use the WT-1 (H-1) antibody to stain for podocytes in kidney organoids. Do you have any secondary antibody to recommend for fluorescence microscopy?

Asked by: ValeM
Thank you for your question. Here is a link to our secondary antibodies: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2019-05-21

Hi, can I ask for the antibody WT1 (H1) insert. Actually I'm looking for WT1 which detect carboxyl Terminus or C-Terminus. Is this is the correct one? to rule out DSRCT (Desmoplastic Small Round Cell Tumours). Can I have the sample for evaluation? TQ

Asked by: RUZA
Thank you for your question. This mouse monoclonal IgM antibody is specific for an epitope mapping between amino acids 123-164 within an internal region of WT1 of human origin. It does not specifically recognize the C-terminus. We do not currently offer any WT1 antibodies with C-terminal epitopes. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-03-03

I am using WT1 (H-1): sc-393498 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6.0) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 15
Date published: 2017-01-20
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Rated 4 out of 5 by from Works with problemsIt works, but there is always a prominent second band. The second band is very close to the band for WT1 and is thicker and more intense than the WT1 band.
Date published: 2022-08-02
Rated 1 out of 5 by from Non funziona su tessuti FFPE di gattoHo ordinato un sample di questo anticorpo per marcare tessuti di gatto fissati in formalina e inclusi in paraffina. Purtroppo non ho riscontrato nessun segnale. Campioni usati: rene fetale con glomeruli immaturi, mesotelioma, nefroblastoma
Date published: 2021-12-28
Rated 1 out of 5 by from 免疫組織化学でうまく機能しない精巣のパラフィン切片を使って免疫蛍光染色を行いましたが、 セルトリ細胞の核が全く染色されませんでした。
Date published: 2020-12-23
Rated 5 out of 5 by from Dr SadekAb work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Date published: 2018-11-21
Rated 1 out of 5 by from No IHC or IF staining at all.We acquire a sample and perform IHC and IF on 4um mouse kidney section with M.O.M. kit. None of them works. Podocytes does not have nuclear staining of WT-1, nor has cytoplasmic staining. It`s just completely negative. I really hope the santa Cruz can resume C-19 (sc-192) since it works perfectly on frozen sections with 4% PFA fixation of 20min.
Date published: 2018-08-23
Rated 1 out of 5 by from WT1 antibody Sc-393498Purchased this antibody recently. Tried it on WB and IP-MS experiments. Didn't work in either. :((
Date published: 2018-05-07
Rated 1 out of 5 by from 染的都是背景I applied a sample sack in Sep. and then I did IF in both testis and heart. Results showed that the nonspecific staining as it stained in cytoplasm and myocardiocytes where supposed not be appear.
Date published: 2017-10-12
Rated 5 out of 5 by from Good, BUTAntibody is perfect, but it maybe unsuitable to replace C-19, because my aim is detect EWS-WT1. The service of SCBT is very nice!
Date published: 2017-07-31
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WT1 抗体 (H-1) is rated 3.1 out of 5 by 14.
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