Date published: 2025-9-7

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WDR1 抗体 (B-10): sc-393159

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说明书
  • WDR1 抗体 B-10 是小鼠单克隆 IgG2a κ,WDR1抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列425-606位于human物种的WDR1的C-terminus
  • WDR1 抗体 (B-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 WDR1
  • 抗WDR1抗体(B-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取WDR1 (B-10): sc-393159免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 WDR1 抗体 (B-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与WDR1 抗体 (B-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

WDR1抗体(B-10)是一种IgG2a κ小鼠单克隆WDR1抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的WDR1蛋白。WDR1抗体(B-10)既可作为非偶联抗WDR1抗体形式提供,也可作为多种偶联形式的抗WDR1抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。WD重复序列是在各种蛋白质中发现的模体,其特点是由40-60个氨基酸组成的保守核心,通常形成三级螺旋桨结构。含有WD重复序列的蛋白质参与广泛的细胞功能,它们通常涉及信号转导、细胞凋亡、转录调控、细胞周期控制等调节机制。WD重复序列作为蛋白质-蛋白质相互作用的位点,有些似乎介导蛋白质复合物的组装。WDR1(WD重复结构域1)含有11个WD重复序列,也被称为AIP1或NORI-1,是一种由606个氨基酸组成的蛋白质,定位于细胞骨架,是WD重复AIP1家族的成员。WDR1以两种可选择性剪接的异构体形式存在,与ADF/cofilin家族蛋白质结合,诱导肌动蛋白丝的解聚。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

WDR1 抗体 (B-10) 参考文献:

  1. PC12 大鼠嗜铬细胞瘤细胞中 WD40 重复 1 蛋白的亚细胞定位。  |  Shin, DH., et al. 2004. Neurosci Lett. 367: 399-403. PMID: 15337274
  2. AIP1/WDR1 支持有丝分裂的细胞整圆。  |  Fujibuchi, T., et al. 2005. Biochem Biophys Res Commun. 327: 268-75. PMID: 15629458
  3. Monad是一种WD40重复蛋白,能促进TNF-α诱导的细胞凋亡。  |  Saeki, M., et al. 2006. Biochem Biophys Res Commun. 342: 568-72. PMID: 16487927
  4. cofilin 伙伴 Aip1/Wdr1 的突变会导致自身炎症性疾病和大血小板减少症。  |  Kile, BT., et al. 2007. Blood. 110: 2371-80. PMID: 17515402
  5. 肌动蛋白相互作用蛋白 1 在哺乳动物细胞的细胞分裂和趋化迁移中的关键作用。  |  Kato, A., et al. 2008. Biochem J. 414: 261-70. PMID: 18494608
  6. 鸣禽基底乳头中存在 WDR1。  |  Adler, HJ., et al. 2008. Hear Res. 240: 102-11. PMID: 18514449
  7. WDR1 在正常和噪声损伤小鸡前庭中的表达。  |  Suh, MW., et al. 2007. J Vestib Res. 17: 163-70. PMID: 18525142
  8. cofilin, coronin 和 Aip1 对肌动蛋白的分解是以爆发的形式进行的,并受到倒钩末端封端器的抑制。  |  Kueh, HY., et al. 2008. J Cell Biol. 182: 341-53. PMID: 18663144
  9. AIP1-缔合作用对肌动蛋白的破坏速度比 Arp2/3 复合物在片状结构中的成核速度快一个数量级。  |  Tsuji, T., et al. 2009. PLoS One. 4: e4921. PMID: 19290054
  10. 正常和噪音受损的Sprague-Dawley大鼠耳蜗中的WDR1表达。  |  Song, JJ., et al. 2013. J Comp Neurol. 521: 1470-81. PMID: 22821633
  11. WDR1 通过 ADF/cofilin 介导的肌动蛋白动力学促进细胞生长和迁移并促成非小细胞肺癌的恶性表型  |  Yuan, B., et al. 2018. Int J Biol Sci. 14: 1067-1080. PMID: 29989053
  12. 敲除 Wdr1 会导致成年小鼠心脏肥大和心脏功能受损。  |  Huang, X., et al. 2019. Gene. 697: 40-47. PMID: 30794912

订购信息

产品名称产品编号规格价格数量收藏夹

WDR1 抗体 (B-10)

sc-393159
200 µg/ml
RMB2377.00

WDR1 (B-10): m-IgG Fc BP-HRP 套装

sc-530375
200 µg Ab; 10 µg BP
RMB2662.00

WDR1 (B-10): m-IgGκ BP-HRP 套装

sc-523829
200 µg Ab, 40 µg BP
RMB2662.00

WDR1 (B-10): m-IgG2a BP-HRP 套装

sc-547489
200 µg Ab; 10 µg BP
RMB2662.00

WDR1 抗体 (B-10) AC

sc-393159 AC
500 µg/ml, 25% agarose
RMB3129.00

WDR1 抗体 (B-10) HRP

sc-393159 HRP
200 µg/ml
RMB2377.00

WDR1 抗体 (B-10) FITC

sc-393159 FITC
200 µg/ml
RMB2482.00

WDR1 抗体 (B-10) PE

sc-393159 PE
200 µg/ml
RMB2580.00

WDR1 抗体 (B-10) Alexa Fluor® 488

sc-393159 AF488
200 µg/ml
RMB2685.00

WDR1 抗体 (B-10) Alexa Fluor® 546

sc-393159 AF546
200 µg/ml
RMB2685.00

WDR1 抗体 (B-10) Alexa Fluor® 594

sc-393159 AF594
200 µg/ml
RMB2685.00

WDR1 抗体 (B-10) Alexa Fluor® 647

sc-393159 AF647
200 µg/ml
RMB2685.00

WDR1 抗体 (B-10) Alexa Fluor® 680

sc-393159 AF680
200 µg/ml
RMB2685.00

WDR1 抗体 (B-10) Alexa Fluor® 790

sc-393159 AF790
200 µg/ml
RMB2685.00

I am using WDR1 (B-10): sc-393159 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_7, h_4CST
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Rated 5 out of 5 by from Good clean antibodyI received this antibody as a free sample and fortunately it worked on the first try. I used it at 1:500 as the sample given only had 50 ul. The recommended 1:100 would be 100 ul for 10 ml. Using their anti-Igkappa-HRP, it gave clean bands. See figure legend for details.
Date published: 2017-10-16
Rated 4 out of 5 by from It is a very good antibody!The quality is very good, but I was dissapointed to find out that I did not receive the quantity written on the vial. On the vial it is written 10micrograms in 50 microL, but I only received 20microL. I was not enough for all my samples. Another colleague of mine also received 30 microL instead od 50microL.
Date published: 2017-09-18
Rated 5 out of 5 by from STRONG BAND In WESTERNI got the sample antibody and tried it in both HT-29 and HT-22 cell lysates. Both show strong bands.
Date published: 2016-11-14
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic stainingBeautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skin tissue. -SCBT QC
Date published: 2015-02-08
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WDR1 抗体 (B-10) is rated 4.8 out of 5 by 4.
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