Date published: 2025-9-6
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VRK1 Antibody (A-11) - Western Blotting - Image 360670
VRK1 抗体 (A-11): sc-271061. Jurkat (A), MCF7 (B), HL-60 (C) 和 HCT-116 (D) 全细胞裂解液中 VRK1 表达的 Western 印迹分析.
VRK1 抗体 (A-11): sc-271061. Jurkat (A), MCF7 (B), HL-60 (C) 和 HCT-116 (D) 全细胞裂解液中 VRK1 表达的 Western 印迹分析.

VRK1 抗体 (A-11): sc-271061

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说明书
  • VRK1 抗体 A-11 是小鼠单克隆 IgG1 κ，VRK1抗体, 在2篇文献中引用，规格为200 µg/ml
  • 针对VRK1的内部区域human起源的肽图
  • VRK1 抗体 (A-11) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 VRK1; 也和以下物种反应，包括： bovine
  • 抗VRK1抗体（A-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VRK1 (A-11): sc-271061的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是VRK1 Antibody (A-11) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与VRK1 Antibody (A-11) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外VRK抗体，包括VRK1, VRK2和VRK3

VRK1抗体（A-11）是一种IgG1κ型小鼠单克隆VRK1抗体（也称为VRK1抗体），可以通过WB、IP、IF、IHC（P）和ELISA等方法检测到人类来源的VRK1蛋白。VRK1抗体（A-11）可以以非共轭型抗VRK1抗体形式获得，也可以以多种共轭型抗VRK1抗体形式获得，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。人类痘苗病毒相关激酶1和2（VRK1,2）是含有NLS的丝氨酸/苏氨酸痘病毒相关激酶，与酪蛋白激酶-1家族成员相似。这些VRK激酶磷酸化与应激反应相关的转录因子，如p53。作为p53的上游调节剂，VRK-1能够磷酸化磷丝蛋白、酪蛋白、组蛋白2b和髓鞘碱性蛋白。VRK1与ATF2在细胞核中共定位，并能形成稳定的复合物。VRK1主要在Thr 73处磷酸化ATF2，稳定ATF2蛋白并增加其细胞内水平。VRK1在Thr18处磷酸化人类p53，并在体外破坏p53-Mdm2相互作用。VRK1在体外磷酸化c-Jun的Ser 63和Ser 73（与c-Jun的N端激酶（JNK）靶向的相同残基），并激活c-Jun依赖的转录。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VRK1 抗体 (A-11) 参考文献:

  1. 人类疫苗相关激酶 1（VRK1）会使 p53 肿瘤抑制蛋白的 mdm-2 结合位点内的苏氨酸-18 磷酸化。  |  Lopez-Borges, S. and Lazo, PA. 2000. Oncogene. 19: 3656-64. PMID: 10951572
  2. 哺乳动物疫苗相关激酶家族三个旁系成员的特征。  |  Nichols, RJ. and Traktman, P. 2004. J Biol Chem. 279: 7934-46. PMID: 14645249
  3. 哺乳动物蛋白激酶新家族的成员补充了B1激酶缺陷的疫苗病毒ts突变体的DNA阴性表型。  |  Boyle, KA. and Traktman, P. 2004. J Virol. 78: 1992-2005. PMID: 14747564
  4. 人类疫苗相关激酶 1（VRK1）通过 Thr-73 和 Ser-62 上的新型磷酸化激活 ATF2 的转录活性，并与 JNK 合作。  |  Sevilla, A., et al. 2004. J Biol Chem. 279: 27458-65. PMID: 15105425
  5. c-Jun 被人类疫苗相关激酶 1 (VRK1) 磷酸化及其与 c-Jun 的 N 端激酶 (JNK) 的合作。  |  Sevilla, A., et al. 2004. Oncogene. 23: 8950-8. PMID: 15378002
  6. 人类疫苗相关激酶 1 诱导的 p53 稳定和积累。  |  Vega, FM., et al. 2004. Mol Cell Biol. 24: 10366-80. PMID: 15542844
  7. 蛋白激酶和磷酸酶：蛋白质磷酸化和信号传递的阴与阳。  |  Hunter, T. 1995. Cell. 80: 225-36. PMID: 7834742
  8. 鉴定与疫苗病毒 B1R 激酶结构相似的两种新型人类假定丝氨酸/苏氨酸激酶 VRK1 和 VRK2。  |  Nezu, J., et al. 1997. Genomics. 45: 327-31. PMID: 9344656

订购信息

产品名称产品编号规格价格数量收藏夹

VRK1 抗体 (A-11)

sc-271061
200 µg/ml
RMB2377.00

VRK1 (A-11): m-IgG Fc BP-HRP 套装

sc-529110
200 µg Ab; 10 µg BP
RMB2662.00

VRK1 (A-11): m-IgGκ BP-HRP 套装

sc-521835
200 µg Ab, 40 µg BP
RMB2662.00

VRK1 (A-11): m-IgG1 BP-HRP 套装

sc-543325
200 µg Ab; 20 µg BP
RMB2662.00

VRK1 抗体 (A-11) AC

sc-271061 AC
500 µg/ml, 25% agarose
RMB3129.00

VRK1 抗体 (A-11) HRP

sc-271061 HRP
200 µg/ml
RMB2377.00

VRK1 抗体 (A-11) FITC

sc-271061 FITC
200 µg/ml
RMB2482.00

VRK1 抗体 (A-11) PE

sc-271061 PE
200 µg/ml
RMB2580.00

VRK1 抗体 (A-11) Alexa Fluor® 488

sc-271061 AF488
200 µg/ml
RMB2685.00

VRK1 抗体 (A-11) Alexa Fluor® 546

sc-271061 AF546
200 µg/ml
RMB2685.00

VRK1 抗体 (A-11) Alexa Fluor® 594

sc-271061 AF594
200 µg/ml
RMB2685.00

VRK1 抗体 (A-11) Alexa Fluor® 647

sc-271061 AF647
200 µg/ml
RMB2685.00

VRK1 抗体 (A-11) Alexa Fluor® 680

sc-271061 AF680
200 µg/ml
RMB2685.00

VRK1 抗体 (A-11) Alexa Fluor® 790

sc-271061 AF790
200 µg/ml
RMB2685.00

VRK1 (A-11) 中和勝肽

sc-271061 P
100 µg/0.5 ml
RMB511.00

Do you have a picture/data of use of this antibody Western blot in mouse, or mouse tissues?

Asked by: Effie
Thank you for your question. We do not have data for this antibody with mouse samples. This antibody is not recommended for use with mouse samples due to the low homology between the epitope sequence and the target sequence in mouse.
Answered by: Blake J
Date published: 2021-07-19

I used the antibody Sc271061 for Hela cells overexpressing Kinase Dead - VRK1 and the antibody could not recognize the overexpressed protein. Do you know if the antibody was produced against the kinase domain?

Asked by: Robertarrs
Thank you for your question. sc-271061:VRK1 (A-11) was raised against a specific epitope mapping between amino acids 268-297 within an internal region of VRK1 of human origin. If the issue with the experiment persists please contact our technical service for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What application is the blocking peptide sc-271061 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from excellent for WB but use sensitive substrateI got this 2 weeks ago. It worked very well. Also 3years ago, I published a paper using this antibody. (Oncotarget, 2015, Vaccinia-related kinase 1 promotes hepatocellular carcinoma by controlling the levels of cell cycle regulators associated with G1/S transition)
Date published: 2019-02-26
Rated 4 out of 5 by from Works good with some optimizationI recieved a sample and I was able to have good results after some optimization in time of incubation (mininum overnight) and with a high concentration (dilution 1:250). I always have a non specific band upper VRK1. But its reliable antibody (I tested the specifity with Overexpression and Knockout).
Date published: 2018-01-19
Rated 5 out of 5 by from Beautiful stainingThis antibody shows great localization of VRK1 to the nuclear in both IF and IHC.
Date published: 2017-02-02
Rated 5 out of 5 by from Excellent antibody for Western blottingThis product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with lysates from diverse human cancer cell lines. In immunoblots with a mix of HeLa and Jurkat cell lysates, this antibody very strongly detected a ~46 kDa protein, which corresponds to the predicted size of VRK1.
Date published: 2016-10-13
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclearProduced beautiful immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-02
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VRK1 抗体 (A-11) is rated 4.8 out of 5 by 5.
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