Date published: 2025-9-11
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VP16 Antibody (14-5) - Western Blotting - Image 353095
VP16 抗体 (14-5) HRP: sc-7546 HRP. 与 GAL4 蛋白融合的 VP16 全长转录激活结构域 (AD) (氨基酸 412-490) 的直接 Western 印迹分析.
VP16 Antibody (14-5) - Western Blotting - Image 353095
VP16 抗体 (14-5) HRP: sc-7546 HRP. 与 GAL4 蛋白融合的 VP16 全长转录激活结构域 (AD) (氨基酸 412-490) 的直接 Western 印迹分析.
VP16 Antibody (14-5) - Western Blotting - Image 5618
VP16 抗体 (14-5): sc-7546. VP16 的 Western 印迹分析. 与 GAL4 融合的 VP16 全长转录激活结构域 (AD) (氨基酸 412-490) (A), 与 GAL4 融合的 C 端删除的 VP16 AD (氨基酸 412-456) (B), 与 GAL4 融合的 N 端删除的 VP16 AD (氨基酸 452-490) (C) 和 GAL4 蛋白 (D).
VP16 抗体 (14-5) HRP: sc-7546 HRP. 与 GAL4 蛋白融合的 VP16 全长转录激活结构域 (AD) (氨基酸 412-490) 的直接 Western 印迹分析.

VP16 抗体 (14-5): sc-7546

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说明书
  • VP16抗体(14-5)是小鼠单克隆IgG2a κ, 在29篇文献中引用，规格为200 µg/ml
  • 针对VP16的N-terminal转录激活域的氨基酸410-456
  • VP16 抗体 (14-5) 推荐用于 WB, IP 和 IF，检测 VP16 and VP16 fusion proteins
  • 抗VP16抗体（14-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取VP16 (14-5): sc-7546的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是VP16 Antibody (14-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与VP16 Antibody (14-5) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

此外 VP 抗体 包括 VP5

VP16抗体（14-5）是一种小鼠单克隆IgG2a kappa轻链抗体，可在包括蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）在内的各种应用中检测VP16。抗VP16抗体（14-5）有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足不同实验需求。VP16，也称为单纯疱疹病毒蛋白vmw65，在病毒生命周期中扮演着至关重要的角色，它充当转录激活因子，对于病毒复制所需的早期基因的表达至关重要。VP16的这一功能尤为重要，因为它使病毒能够劫持宿主的细胞机制，从而促进病毒的高效繁殖和发病。了解VP16的相互作用和机制有助于我们找到治疗疱疹病毒感染的潜在靶点。双杂交系统是一种广泛使用的检测蛋白质-蛋白质相互作用的方法，可用于研究VP16与其他蛋白质的相互作用，从而加深我们对VP16在病毒生命周期中的作用以及对宿主细胞功能的影响的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VP16 抗体 (14-5) 参考文献:

  1. 在单纯疱疹病毒感染过程中，染色质修饰辅助激活因子与组蛋白在即刻早期基因启动子上的代表性不足的关联依赖于 VP16。  |  Herrera, FJ. and Triezenberg, SJ. 2004. J Virol. 78: 9689-96. PMID: 15331701
  2. Gal4-VP16 在酵母菌染色质组装系统中指导 ATP 依赖性染色质重组。  |  Robinson, KM. and Schultz, MC. 2005. Biochemistry. 44: 4551-61. PMID: 15766286
  3. VP16 激活域在体内建立了缺乏 CDK8 的活性介质。  |  Uhlmann, T., et al. 2007. J Biol Chem. 282: 2163-73. PMID: 17135252
  4. Mediator 中 VP16 反式激活因子靶标的结构。  |  Milbradt, AG., et al. 2011. Nat Struct Mol Biol. 18: 410-5. PMID: 21378963
  5. VP16 丝氨酸 375 是体内单纯疱疹病毒脱离潜伏期的关键决定因素。  |  Sawtell, NM., et al. 2011. J Neurovirol. 17: 546-51. PMID: 22144074
  6. GAL4-VP16 是一种异常强大的转录激活物。  |  Sadowski, I., et al. 1988. Nature. 335: 563-4. PMID: 3047590
  7. 成人 Ph+ ALL 患者可从中度剂量 VP16 加 CY/TBI 的治疗方案中获益。  |  Morita-Fujita, M., et al. 2022. Hematol Oncol. 40: 1041-1055. PMID: 35790020
  8. 应激诱导转录因子 Slug 可刺激单纯疱疹病毒 1 的复制并转录 VP16 启动子内的顺式调控模块。  |  Santos, VC., et al. 2023. J Virol. 97: e0007323. PMID: 37022165
  9. 神经元表达单纯疱疹病毒-1 VP16 蛋白可诱导伪狂犬病毒摆脱沉默和重新激活。  |  Hsu, Z-S., et al. 2024. J Virol. 98: e0056124. PMID: 38869285
  10. 利用疱疹病毒 VP16 转座子增强哺乳动物细胞中重组蛋白的表达。  |  Hippenmeyer, PJ. and Pegg, LE. 1995. Curr Opin Biotechnol. 6: 548-52. PMID: 7579666

订购信息

产品名称产品编号规格价格数量收藏夹

VP16 抗体 (14-5)

sc-7546
200 µg/ml
RMB2377.00

VP16 (14-5): m-IgG Fc BP-HRP 套装

sc-528183
200 µg Ab; 10 µg BP
RMB2662.00

VP16 (14-5): m-IgGκ BP-HRP 套装

sc-520504
200 µg Ab, 40 µg BP
RMB2662.00

VP16 (14-5): m-IgG2a BP-HRP 套装

sc-547039
200 µg Ab; 10 µg BP
RMB2662.00

VP16 抗体 (14-5) AC

sc-7546 AC
500 µg/ml, 25% agarose
RMB3129.00

VP16 抗体 (14-5) HRP

sc-7546 HRP
200 µg/ml
RMB2377.00

VP16 抗体 (14-5) FITC

sc-7546 FITC
200 µg/ml
RMB2482.00

VP16 抗体 (14-5) PE

sc-7546 PE
200 µg/ml
RMB2580.00

VP16 抗体 (14-5) Alexa Fluor® 488

sc-7546 AF488
200 µg/ml
RMB2685.00

VP16 抗体 (14-5) Alexa Fluor® 546

sc-7546 AF546
200 µg/ml
RMB2685.00

VP16 抗体 (14-5) Alexa Fluor® 594

sc-7546 AF594
200 µg/ml
RMB2685.00

VP16 抗体 (14-5) Alexa Fluor® 647

sc-7546 AF647
200 µg/ml
RMB2685.00

VP16 抗体 (14-5) Alexa Fluor® 680

sc-7546 AF680
200 µg/ml
RMB2685.00

VP16 抗体 (14-5) Alexa Fluor® 790

sc-7546 AF790
200 µg/ml
RMB2685.00

VP16 抗体 (14-5) B

sc-7546 B
200 µg/ml
RMB2407.00

Hi, Which VP16 fusion proteins (e.g. rtTA3, rtTA-V10, rtTA-V16) can be detected by sc-7545 or sc-7546?

Asked by: kk421
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-01-11

Hi, may I request for the full sequence of the anti-VP16 antibody to verify its binding sequence and downstream mutation potential?

Asked by: Sheena_sing
Thank you for your question. It would be helpful if you could call us directly at 800- 457-3801 option 2, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-04

Will this antibody detect dCas9-VPR (Cas9 activator)?

Asked by: Kumi
Thank you for your question. This mouse monoclonal antibody is recommended for detection of VP16 and VP16 fusion proteins. We have not tested the antibody for detection of dCas9-VPR. If you have the protein sequence for dCas9-VPR, please contact our Technical Service Department and we can assist you with performing sequence analysis using this antibody's antigen to determine potential cross-reactivity with your protein of interest.
Answered by: Technical Support
Date published: 2019-02-25

Hi, I'm looking for an antisera against VP16. Could you please send me a sample of yours for me to test? I'm looking for one against VP-16 expressed transiently in Tobacco ( N. Benthi). I want to test that the antisera will detect this first.

Asked by: YingS
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

Can this antibody be used with cat samples?

Asked by: AbPolly
This antibody can be used with any sample to detect VP16 fusion proteins. For endogenous VP16, please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 3
Date published: 2017-01-12

If I am using sc-7546 for WB, what do you recommend for detection?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-12
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Rated 5 out of 5 by from Strong western dataVery good antibody for western blot; strong signal with low background
Date published: 2017-01-17
Rated 5 out of 5 by from Excellent for fusion protein detection.If you're working with a VP16 fusion protein, this antibody will detect it. It delivers strong WB results, but will work great in your IP and IF as well. 12 citations support it!
Date published: 2016-12-23
Rated 4 out of 5 by from WB data obtained was good but we have seenWB data obtained was good but we have seen better with other VP16 antibodies from SCBT.
Date published: 2015-09-28
Rated 5 out of 5 by from Publishable WB data with transfected Hela and 293TPublishable WB data with transfected Hela and 293T, SW-480, and COS7 cells - SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for CoIP on Cos7 and Vero cells - SCBT Publication Review
Date published: 2015-03-11
Rated 5 out of 5 by from Successful application of this antibody inSuccessful application of this antibody in IF and WB using monkey scFvs-VP16 transfected COS7 cells, and scFvs mammalian cells -SCBT Publication Review
Date published: 2015-03-10
Rated 5 out of 5 by from ChIP publishable data with HeLaChIP publishable data with HeLa, NCI-H226, HCT116, HCT116 DNMT3B KO and DNMT1/DNMT3B DKO cells - SCBT Publication Review
Date published: 2014-03-12
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VP16 抗体 (14-5) is rated 4.9 out of 5 by 7.
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