Date published: 2025-9-11
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VP16 Antibody (1-21) - Western Blotting - Image 5624
VP16 抗体 (1-21): sc-7545. VP16 的 Western 印迹分析. 与 GAL4 融合的 VP16 全长转录激活结构域 (AD) (氨基酸 412-490) (A), 与 GAL4 融合的 C 端删除的 VP16 AD (氨基酸 412-456) (B), 与 GAL4 融合的 N 端删除的 VP16 AD (氨基酸 452-490) (C) 和 GAL4 蛋白 (D).
VP16 Antibody (1-21) - Western Blotting - Image 5624
VP16 抗体 (1-21): sc-7545. VP16 的 Western 印迹分析. 与 GAL4 融合的 VP16 全长转录激活结构域 (AD) (氨基酸 412-490) (A), 与 GAL4 融合的 C 端删除的 VP16 AD (氨基酸 412-456) (B), 与 GAL4 融合的 N 端删除的 VP16 AD (氨基酸 452-490) (C) 和 GAL4 蛋白 (D).
VP16 抗体 (1-21): sc-7545. VP16 的 Western 印迹分析. 与 GAL4 融合的 VP16 全长转录激活结构域 (AD) (氨基酸 412-490) (A), 与 GAL4 融合的 C 端删除的 VP16 AD (氨基酸 412-456) (B), 与 GAL4 融合的 N 端删除的 VP16 AD (氨基酸 452-490) (C) 和 GAL4 蛋白 (D).

VP16 抗体 (1-21): sc-7545

4.8(5)
寫評論提問

说明书
  • VP16 抗体 1-21 是小鼠单克隆 IgG1 κ，VP16抗体, 在112篇文献中引用，规格为200 µg/ml
  • 针对氨基酸456-490的重组VP16
  • VP16 抗体 (1-21) 推荐用于 WB, IP 和 IF，检测 VP16 and VP16 fusion proteins
  • 抗VP16抗体（1-21）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取VP16 (1-21): sc-7545的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是VP16 Antibody (1-21) for WB applications. 的首选辅助检测试剂，这些试剂现与VP16 Antibody (1-21) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

此外 VP 抗体 包括 VP5

VP16抗体（1-21）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测VP16蛋白。抗VP16抗体（1-21）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。VP16蛋白，也称为1型单纯疱疹病毒（HSV-1）的65 kDa包膜蛋白，在病毒生命周期中起着至关重要的作用，它促进启动病毒复制所必需的早期基因的转录。VP16 (1-21) 单克隆抗体与宿主转录因子（如Oct-1和宿主细胞因子1（HCF））相互作用，增强病毒基因的转录活性，从而促进病毒的高效复制和发病。VP16能够调节宿主细胞机制，这凸显了其在病毒学中的重要性，并突出了针对该蛋白进行干预治疗的潜力。双杂交系统是一种广泛使用的检测蛋白质-蛋白质相互作用的方法，利用VP16的独特性质，使研究人员能够探索复杂的蛋白质相互作用及其在各种生物过程中的意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VP16 抗体 (1-21) 参考文献:

  1. 用于在酵母或哺乳动物细胞中表达的 GAL4 融合载体。  |  Sadowski, I., et al. 1992. Gene. 118: 137-41. PMID: 1511877
  2. 双杂交系统：一种识别和克隆与感兴趣的蛋白质相互作用的蛋白质基因的方法。  |  Chien, CT., et al. 1991. Proc Natl Acad Sci U S A. 88: 9578-82. PMID: 1946372
  3. 酵母 STE12 蛋白的信息素依赖性磷酸化与转录激活有关。  |  Song, D., et al. 1991. Genes Dev. 5: 741-50. PMID: 2026326
  4. 激活剂和目标。  |  Ptashne, M. and Gann, AA. 1990. Nature. 346: 329-31. PMID: 2142753
  5. 检测蛋白质-蛋白质相互作用的新型基因系统。  |  Fields, S. and Song, O. 1989. Nature. 340: 245-6. PMID: 2547163
  6. 真菌基因调控机制模型：酿酒酵母的 GAL 基因。  |  Johnston, M. 1987. Microbiol Rev. 51: 458-76. PMID: 2830478
  7. GAL4 的缺失分析确定了两个转录激活区段。  |  Ma, J. and Ptashne, M. 1987. Cell. 48: 847-53. PMID: 3028647
  8. 鸭肠炎病毒 VP16 可拮抗 IFN-β 介导的抗病毒先天免疫力  |  Li, Y., et al. 2020. J Immunol Res. 2020: 9630452. PMID: 32537474
  9. VP16 在阿尔法疱疹病毒生命周期中的作用  |  Fan, D., et al. 2020. Front Microbiol. 11: 1910. PMID: 33013729
  10. 与 VP16 或 VP16 诱导的复合体相关的宿主因素对 HSV-1 溶菌感染有不同的影响。  |  Ding, X., et al. 2022. Rev Med Virol. 32: e2394. PMID: 36069169
  11. VP16-213 和 podophyllotoxin。化学结构与生物活性之间关系的研究。  |  Loike, JD. 1982. Cancer Chemother Pharmacol. 7: 103-11. PMID: 7044591
  12. 双杂交系统：蛋白质-蛋白质相互作用的检测方法。  |  Fields, S. and Sternglanz, R. 1994. Trends Genet. 10: 286-92. PMID: 7940758

订购信息

产品名称产品编号规格价格数量收藏夹

VP16 抗体 (1-21)

sc-7545
200 µg/ml
RMB2377.00

VP16 (1-21): m-IgG Fc BP-HRP 套装

sc-526503
200 µg Ab; 10 µg BP
RMB2662.00

VP16 (1-21): m-IgG1 BP-HRP 套装

sc-531876
200 µg Ab; 20 µg BP
RMB2662.00

VP16 抗体 (1-21) AC

sc-7545 AC
500 µg/ml, 25% agarose
RMB3129.00

VP16 抗体 (1-21) HRP

sc-7545 HRP
200 µg/ml
RMB2377.00

VP16 抗体 (1-21) FITC

sc-7545 FITC
200 µg/ml
RMB2482.00

VP16 抗体 (1-21) PE

sc-7545 PE
200 µg/ml
RMB2580.00

VP16 抗体 (1-21) Alexa Fluor® 488

sc-7545 AF488
200 µg/ml
RMB2685.00

VP16 抗体 (1-21) Alexa Fluor® 546

sc-7545 AF546
200 µg/ml
RMB2685.00

VP16 抗体 (1-21) Alexa Fluor® 594

sc-7545 AF594
200 µg/ml
RMB2685.00

VP16 抗体 (1-21) Alexa Fluor® 647

sc-7545 AF647
200 µg/ml
RMB2685.00

VP16 抗体 (1-21) Alexa Fluor® 680

sc-7545 AF680
200 µg/ml
RMB2685.00

VP16 抗体 (1-21) Alexa Fluor® 790

sc-7545 AF790
200 µg/ml
RMB2685.00

VP16 抗体 (1-21) TRITC

sc-7545 TRITC
200 µg/ml
RMB2407.00

I am interested in working with this VP16 antibody for western blotting. I would like to know if in order to prepare the antibody, this is resuspended in milk or bsa, as it is not specified in the datasheet or the web page.

Asked by: Anonymous
Thank you for your question. We recommend using UltraCruz® Blocking Reagent: sc-516214 https://www.scbt.com/p/ultracruz-blocking-reagent for blocking purposes. This reagent is also what is used for our tests wen establishing antibody stainings.
Answered by: Tech Support Europe
Date published: 2025-02-10

这里有2个不同分子量的 一般是检测哪个？

Asked by: Jummy
Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Yanru Tao
Date published: 2024-08-26

Is the antibody sc-7545 able to recognize VP64 protein (4 times epitope 437-447 of VP16 protein)?

Asked by: enzoelena
Thank you for your question. VP16 Antibody (1-21): sc-7545 was raised against amino acids 456-490 of VP16 and will recognize proteins containing this exact amino acid range. If your particular protein or peptide only contains amino acid range 437-447 it will not be recognized by this antibody.
Answered by: Tech Support Europe
Date published: 2023-07-17

Hi. Can I request a sample for sc-7545? I have a VP16 fused GAL4 protein and I want to check its expression by western blot and IF. This antibody is suitable for my purpose but firstly I need to check if it is working with my fused protein. Thank you,

Asked by: Huseyin
Thank you for your question. Please contact our Technical Service Department (or your local Distributor) directly to arrange for a 10 µg sample of VP16 (1-21): sc-7545.
Answered by: Tech Support Europe
Date published: 2022-01-11

What is the recommended secondary reagent to use with VP16 Antibody (1-21): sc-7545 for IF?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins (m-IgGκ BP) conjugated to either FITC, PE or a CruzFluor as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 4 out of 5 by from good for WB of HSV1-infected cellsworked well (1:1000 dilution) for detection of HSV1 VP16 in infected 293T cell lysates by western blot.
Date published: 2017-11-30
Rated 5 out of 5 by from Excellent for Western blotsGives strong Western blot signals with very little/no background. It helped us see that one of four VP16 fusion proteins was not expressing well after transfection of Drosophila cells.
Date published: 2017-05-23
Rated 5 out of 5 by from Outstanding western dataVery strong western blot signal; little to no background
Date published: 2017-01-04
Rated 5 out of 5 by from Great for detecting VP16!A proven detector of VP16 and VP16 fusion proteins. It works well in all standard applications and will provide reliable results with strong and specific signal. This antibody has been published with nearly 70 times.
Date published: 2017-01-03
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VP16 抗体 (1-21) is rated 4.8 out of 5 by 5.
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