Date published: 2025-9-7
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VIP Antibody (H-6) - Western Blotting - Image 384625
VIP 抗体 (H-6): sc-25347. 福尔马林固定, 石蜡包埋的人结肠组织的免疫过氧化物酶染色, 显示细胞外染色.
VIP 抗体 (H-6): sc-25347. 福尔马林固定, 石蜡包埋的人结肠组织的免疫过氧化物酶染色, 显示细胞外染色.

VIP 抗体 (H-6): sc-25347

4.9(11)
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说明书
  • VIP 抗体 H-6 是小鼠单克隆 IgG2b κ，VIP抗体, 在38篇文献中引用，规格为200 µg/ml
  • 免疫human物种的vasoactive intestinal peptide (VIP)的氨基酸1-95
  • VIP 抗体 (H-6) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 VIP
  • 抗-VIP 抗体 (H-6) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VIP (H-6): sc-25347的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 VIP 抗体 (H-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与VIP 抗体 (H-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

VIP抗体（H-6）是一种IgG2b κ小鼠单克隆VIP抗体（也称为胰高血糖素（血管扩张诱导）家族成员抗体、血管活性肠多肽（VIP）抗体、28个氨基酸胰高血糖素/分泌素超家族配体抗体、G蛋白偶联受体（GPCR）VPAC1/VPAC2配体抗体或PHM27抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人类来源的VIP蛋白。VIP抗体（H-6）既可作为非偶联抗VIP抗体形式，也可作为多种偶联形式的抗VIP抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。胰高血糖素是一种胰腺激素，可作为胰岛素的拮抗剂，刺激糖原向葡萄糖转化，提高血糖水平。胰高血糖素样肽-1（GLP-1）、胰高血糖素样肽-2（GLP-2）、VIP（血管活性肠肽）和PACAP（垂体腺苷酸环化酶激活多肽）是胰高血糖素激素家族的成员。GLP-1在中枢神经系统中起传递作用，抑制摄食和饮水行为，而GLP-2是肠上皮生长的促进剂。VIP引起血管扩张，导致血压降低。PACAP在下丘脑中含量丰富，已证明可增加多种激素的合成，包括生长激素。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VIP 抗体 (H-6) 参考文献:

  1. 胰高血糖素经枯草蛋白样原激素转化酶 PC2 和 PC3 的不同处理，生成胰高血糖素或胰高血糖素样肽。  |  Rouillé, Y., et al. 1995. J Biol Chem. 270: 26488-96. PMID: 7592866
  2. 大鼠胰岛细胞中胰高血糖素, 胰高血糖素样肽 I 和葡萄糖依赖性促胰岛素肽受体的表达和功能活性。  |  Moens, K., et al. 1996. Diabetes. 45: 257-61. PMID: 8549871
  3. 胰高血糖素样肽 1 受体基因发生无效突变的小鼠葡萄糖耐受不良，但饱腹感正常。  |  Scrocchi, LA., et al. 1996. Nat Med. 2: 1254-8. PMID: 8898756
  4. 血管活性肠肽（VIP）可刺激带有 VIP-1 受体的人胰腺腺癌衍生物细胞体外生长。  |  Jiang, S., et al. 1997. Cancer Res. 57: 1475-80. PMID: 9108448
  5. 肝脏中糖原代谢的具体特征。  |  Bollen, M., et al. 1998. Biochem J. 336 (Pt 1): 19-31. PMID: 9806880
  6. 垂体腺苷酸环化酶激活多肽（PACAP）38 和 PACAP27 激活共同但不同的细胞内信号通路，刺激猪体细胞分泌生长激素。  |  Martínez-Fuentes, AJ., et al. 1998. Endocrinology. 139: 5116-24. PMID: 9832451

订购信息

产品名称产品编号规格价格数量收藏夹

VIP 抗体 (H-6)

sc-25347
200 µg/ml
RMB2377.00

VIP (H-6): m-IgGκ BP-HRP 套装

sc-520782
200 µg Ab, 40 µg BP
RMB2662.00

VIP (H-6): m-IgG2b BP-HRP 套装

sc-548853
200 µg Ab; 10 µg BP
RMB2662.00

VIP 抗体 (H-6) AC

sc-25347 AC
500 µg/ml, 25% agarose
RMB3129.00

VIP 抗体 (H-6) HRP

sc-25347 HRP
200 µg/ml
RMB2377.00

VIP 抗体 (H-6) FITC

sc-25347 FITC
200 µg/ml
RMB2482.00

VIP 抗体 (H-6) PE

sc-25347 PE
200 µg/ml
RMB2580.00

VIP 抗体 (H-6) Alexa Fluor® 488

sc-25347 AF488
200 µg/ml
RMB2685.00

VIP 抗体 (H-6) Alexa Fluor® 546

sc-25347 AF546
200 µg/ml
RMB2685.00

VIP 抗体 (H-6) Alexa Fluor® 594

sc-25347 AF594
200 µg/ml
RMB2685.00

VIP 抗体 (H-6) Alexa Fluor® 647

sc-25347 AF647
200 µg/ml
RMB2685.00

VIP 抗体 (H-6) Alexa Fluor® 680

sc-25347 AF680
200 µg/ml
RMB2685.00

VIP 抗体 (H-6) Alexa Fluor® 790

sc-25347 AF790
200 µg/ml
RMB2685.00

Hi, does this antibody work with IHC on free floating sections from mouse?

Asked by: Dakota
Thank you for your question. For assistance please contact Technical service . Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-07-28

I understand the antibody is supplied at a concentration of 200ug/ml but I don't see information on the VOLUME supplied per tube - is it 50ul?

Asked by: ebittman
Thank you for your question. As indicated on the product datasheet, this antibody is supplied as 200 µg in 1 ml PBS with 0.1 % gelatin and <0.1 % sodium azide.
Answered by: Tech Support Europe
Date published: 2021-09-13

Did you test this antibody in flowcytometry ?

Asked by: Or1234
Thank you for your question. This antibody has not yet been tested for Flow cytometry so that is not a recommended application at this time. The conjugated form is recommended and warrantied for Flow cytometry use, although we are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2020-02-10

Please confirm that this is raised against 1-95 of preproVIP and provide the sequence.  VIP is 28 aa on the end of preproVIP and corresponds to 125-152 of that molecule. This is raised against 1-95 and and  NOT 125-152 of preproVIP, correct?

Asked by: Susan81
Thank you for your question. Yes, this antibody was raised against amino acids 1-95 of VIP of human origin (accession#P01282).
Answered by: Tech Service
Date published: 2019-08-13

Can this antibody be used in mouse corneal immunofluorescence staining?

Asked by: fff993541
Thank you for your question. We have not tested this antibody in mouse samples, and so this is not a recommended species at this time.
Answered by: Technical Support
Date published: 2019-02-13

does it work for fluorescence immunohistochemistry ?

Asked by: abii
Thanks for your question. Yes, the antibody is recommended for detection of VIP of human origin by WB, IP, IF, IHC(P) and ELISA. Please contact Technical service for further support.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Was this antibody ever tested for mouse VIP? Also, the website says that this antibody is against aa 1-95 ?? as VIP is a 28aa peptide what was VIP conjugated to?

Asked by: ValerieC
Thank you for the question. This antibody is recommended for human tissue samples and it did not show good reactivity on mouse samples in our tests. The WB image was done with the VIP fused to a protein tag and the mol. weight indicated in this image does not refelect the mol. weight. of the endogenously expressed VIP protein. This monoclonal antibody was raised against an antigen consisting of amino acids 1-95 of VIP of human origin with protein accession number P01282, as shown also on our corresponding product website MDTRNKAQLLVLLTLLSVLFSQTSAWPLYRAPSALRLGDRIPFEGANEPDQVSLKEDIDMLQNALAENDTPYYDVSRNARHADGVFTSDFSKLLG
Answered by: Technical Support Europe
Date published: 2018-01-10

Can VIP (H-6): sc-25347 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: cjMara
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. VIP (H-6): sc-25347 monoclonal antibody is currently available conjugated to PE, FITC, Alexa Fluor 488, and Alexa Fluor 647.
Answered by: Technical Support 15
Date published: 2017-01-22
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Rated 5 out of 5 by from Good antibody for IFGood conjugated antibody for IF staining on mouse colon tissues (frozen sections).
Date published: 2024-11-07
Rated 5 out of 5 by from Ottimo anticorpoAbbiamo utilizzato questo anticorpo su sezioni in paraffina, senza smascheramento e con un'incubazione di un'ora a RT, il risultato è stato molto buono e con un'elevata specificità.
Date published: 2023-02-02
Rated 5 out of 5 by from It looked greatI ordered this a month ago and it worked great! I will purchase again
Date published: 2018-05-04
Rated 5 out of 5 by from Very GoodGreat for immunofluorescence in formalin-fixed, paraffin-embedded human skin tissue
Date published: 2018-01-31
Rated 5 out of 5 by from GoodThe analysis results of WB were very stong and clear, it worked well for WB
Date published: 2017-06-08
Rated 5 out of 5 by from Feel like a VIPVIP (H-6) is well cited for IF and IHC(P). Gives a strong staining in human colon tissue showing extracellular localization.
Date published: 2017-01-11
Rated 4 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of human recombinant VIP fusion protein. -SCBT QC
Date published: 2015-07-08
Rated 5 out of 5 by from Worked for western blot using crude extractsWorked for western blot using crude extracts from rat retinas. -SCBT Publication Review
Date published: 2015-06-02
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VIP 抗体 (H-6) is rated 4.9 out of 5 by 11.
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