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Flt-1/VEGFR1 Antibody (D-2) - Immunohistochemistry - Image 354229
VEGFR1 抗体 (D-2): sc-271789. 福尔马林固定, 石蜡包埋的人胎盘组织的免疫过氧化物酶染色显示蜕膜细胞的胞浆染色.
VEGFR1 抗体 (D-2): sc-271789. 福尔马林固定, 石蜡包埋的人胎盘组织的免疫过氧化物酶染色显示蜕膜细胞的胞浆染色.

Flt-1/VEGFR1 抗体 (D-2): sc-271789

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寫評論提問

说明书
  • Flt-1/VEGFR1 抗体 D-2 是小鼠单克隆 IgG1 κ，Flt-1/VEGFR1抗体, 在27篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Flt-1的氨基酸23-247
  • Flt-1/VEGFR1 抗体 (D-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 VEGFR1
  • 抗Flt-1/VEGFR1抗体（D-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Flt-1/VEGFR1 (D-2): sc-271789的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 Flt-1/VEGFR1 抗体 (D-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Flt-1/VEGFR1 抗体 (D-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 VEGFR / Flt 抗体 包括 Flt-4

Flt-1抗体（D-2）是一种IgG1 κ小鼠单克隆Flt-1抗体（也被指定为FLT抗体、fms相关受体酪氨酸激酶1抗体、FLT1抗体），能够通过WB、IP、IF、IHC(P)和ELISA检测到人类来源的Flt-1蛋白。Flt-1抗体（D-2）既有非偶联型抗Flt-1抗体形式，也有多种偶联型抗Flt-1抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。三种细胞膜受体酪氨酸激酶，即Flt-1（也被指定为VEGF-R1）、Flk-1（也被指定为VEGF-R2）和Flt-4，据推测参与内皮细胞的生长，其特征是细胞外结构域中存在七个免疫球蛋白样序列。这些受体彼此之间表现出高度的序列相关性，与III类受体（包括CSF-1/Fms、PDGR、SLFR/Kit和Flt-3/Flk-2）的相关性较小。已显示该受体类别中的两个成员，即Flt-1和Flk-1，代表血管内皮生长因子（VEGFs）的高亲和力受体。基于与Flt-1和Flk-1的结构相似性，推测Flt-4可能代表VEGF或VEGF相关配体的第三个受体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Flt-1/VEGFR1 抗体 (D-2) 参考文献:

  1. fms 样酪氨酸激酶，血管内皮生长因子的受体。  |  de Vries, C., et al. 1992. Science. 255: 989-91. PMID: 1312256
  2. VEGFR1阳性造血骨髓祖细胞启动转移前微环境。  |  Kaplan, RN., et al. 2005. Nature. 438: 820-7. PMID: 16341007
  3. 从富集的原始造血细胞群体中分离出的受体酪氨酸激酶 cDNA，与 c-kit 具有密切的遗传联系。  |  Matthews, W., et al. 1991. Proc Natl Acad Sci U S A. 88: 9026-30. PMID: 1717995
  4. 与 fms 家族密切相关的新型人类受体型酪氨酸激酶基因（flt）的核苷酸序列和表达。  |  Shibuya, M., et al. 1990. Oncogene. 5: 519-24. PMID: 2158038
  5. VEGFR1信号在视网膜血管生成和微炎症中的作用。  |  Uemura, A., et al. 2021. Prog Retin Eye Res. 84: 100954. PMID: 33640465
  6. HIF-1α抑制剂YC-1通过靶向PlGF/VEGFR1诱导的巨噬细胞极化，抑制三阴性乳腺癌的生长和血管生成。  |  Li, Y., et al. 2023. Biomed Pharmacother. 161: 114423. PMID: 36822023
  7. 一种明确的以蛋白酶为介质的运输途径调节内皮细胞分泌的 sFLT1/VEGFR1。  |  Kinghorn, K., et al. 2024. Angiogenesis. 27: 67-89. PMID: 37695358
  8. 靶向NGF而非VEGFR1或BDNF信号可减轻小鼠子宫内膜异位症相关疼痛。  |  Zaninelli, TH., et al. 2024. J Adv Res.. PMID: 39142441
  9. 高亲和力血管内皮生长因子结合和发育表达表明，Flk-1 是血管生成和血管生成的主要调节因子。  |  Millauer, B., et al. 1993. Cell. 72: 835-46. PMID: 7681362
  10. 胚胎发育和组织修复过程中血管内皮生长因子受体的表达表明它在内皮分化和血管生长中发挥作用。  |  Peters, KG., et al. 1993. Proc Natl Acad Sci U S A. 90: 8915-9. PMID: 7692439
  11. FLT4 基因编码一种与血管内皮生长因子受体有关的跨膜酪氨酸激酶。  |  Galland, F., et al. 1993. Oncogene. 8: 1233-40. PMID: 8386825
  12. NYK/FLK-1：一种从 E10 胚胎神经上皮细胞中分离出来的推定受体蛋白酪氨酸激酶，在发育中的胚胎内皮细胞中表达。  |  Oelrichs, RB., et al. 1993. Oncogene. 8: 11-8. PMID: 8423988

订购信息

产品名称产品编号规格价格数量收藏夹

Flt-1/VEGFR1 抗体 (D-2)

sc-271789
200 µg/ml
RMB2377.00

Flt-1/VEGFR1 (D-2): m-IgG Fc BP-HRP 套装

sc-525822
200 µg Ab; 10 µg BP
RMB2662.00

Flt-1/VEGFR1 抗体 (D-2) AC

sc-271789 AC
500 µg/ml, 25% agarose
RMB3129.00

Flt-1/VEGFR1 抗体 (D-2) HRP

sc-271789 HRP
200 µg/ml
RMB2377.00

Flt-1/VEGFR1 抗体 (D-2) FITC

sc-271789 FITC
200 µg/ml
RMB2482.00

Flt-1/VEGFR1 抗体 (D-2) PE

sc-271789 PE
200 µg/ml
RMB2580.00

Flt-1/VEGFR1 抗体 (D-2) Alexa Fluor® 488

sc-271789 AF488
200 µg/ml
RMB2685.00

Flt-1/VEGFR1 抗体 (D-2) Alexa Fluor® 546

sc-271789 AF546
200 µg/ml
RMB2685.00

Flt-1/VEGFR1 抗体 (D-2) Alexa Fluor® 594

sc-271789 AF594
200 µg/ml
RMB2685.00

Flt-1/VEGFR1 抗体 (D-2) Alexa Fluor® 647

sc-271789 AF647
200 µg/ml
RMB2685.00

Flt-1/VEGFR1 抗体 (D-2) Alexa Fluor® 680

sc-271789 AF680
200 µg/ml
RMB2685.00

Flt-1/VEGFR1 抗体 (D-2) Alexa Fluor® 790

sc-271789 AF790
200 µg/ml
RMB2685.00

Can sc-271789 also detect soluble FLT-1 and do you have links to western blot pictures where it has been used. Thanks

Asked by: Toluwalase
Thank you for your question. According to UniProt, soluble Flt-1 (isoforms 2, 3 and 4) are missing C-terminal portions of this protein. This monoclonal antibody recognizes an epitope that is N-terminal and present in these isoforms, so this antibody should be able to recognize soluble Flt-1. However, we have not specifically tested this antibody for detection of the soluble isoforms, and we can not provide such data on our website at this time. You may find some of the product citations using this antibody helpful to see if other researchers have published this type of data with this antibody.
Answered by: Technical Support
Date published: 2019-07-10

Can Flt-1 (D-2): sc-271789 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Flt-1 (D-2): sc-271789 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from it gave some non-specific bandsWe used for WB 1:600 and gave some non-specific bands
Date published: 2021-10-29
Rated 1 out of 5 by from does not workwe tested the antibody with different concentrations using different cell lysate but we couldn't get a well defined band of the protein
Date published: 2021-07-13
Rated 1 out of 5 by from unfortunately does not works in IHCParaffin embedded human jejunal sections (n=6; 5 µm) were rehydrated, antigen retrieval was performed heating sections 25 minutes at 90 °C in a water bath in the presence of 10 mmol / l sodium citrate buffer pH 6.0. Sections were treated with an endogenous peroxidase blocking kit. Immunostaining was performed using a commercial kit following the manufacturer's instructions. Ab Dilution: 0.02 µg/ µl In the jejunum (full thickness) of control samples, the antibody does not work. No signal found in any gastrointestinal layer of the n=6 tested samples. Probably a different antigen retrieval method should be used to expose the epitope.
Date published: 2020-07-01
Rated 1 out of 5 by from Did not work for WB analysisTried at a 1:500 dilution in human and canine bladder cancer cells but detected no signal.
Date published: 2018-06-19
Rated 5 out of 5 by from Gute Ergebnisse IHC-ParaffinHabe den AK 1:100 in Paraffin getestet mit guten Ergebnissen.
Date published: 2018-02-14
Rated 5 out of 5 by from works for WBused for blotting flt-1 transfected cells. worked well!
Date published: 2017-10-03
Rated 1 out of 5 by from IHC Assay Review for Flt-1 mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: No staining observed.
Date published: 2017-08-31
Rated 5 out of 5 by from positive bandThis antibody is very sensitivity for Flt-1 detecion ,we use Hela cell lysates only loading 30ug, but signal appears very strong and specific.
Date published: 2017-08-12
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Flt-1/VEGFR1 抗体 (D-2) is rated 3.7 out of 5 by 14.
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