Date published: 2025-9-6
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VCAM-1 Antibody (M/K-2) - Western Blotting - Image 153709
VCAM-1 抗体 (M/K-2): sc-18864. 在非还原条件下, 对 Sol8 全细胞裂解液中 VCAM-1 的表达进行 Western 印迹分析.
VCAM-1 Antibody (M/K-2) - Western Blotting - Image 153709
VCAM-1 抗体 (M/K-2): sc-18864. 在非还原条件下, 对 Sol8 全细胞裂解液中 VCAM-1 的表达进行 Western 印迹分析.
VCAM-1 Antibody (M/K-2) - Immunohistochemistry - Image 284874
VCAM-1 抗体 (M/K-2): sc-18864. 福尔马林固定, 石蜡包埋的人肾上腺组织的免疫过氧化物酶染色显示腺细胞亚群的胞浆染色.
VCAM-1 Antibody (M/K-2) - Western Blotting - Image 397542
VCAM-1 抗体 (M/K-2) HRP: sc-18864 HRP. 在非还原条件下对 Sol8 全细胞裂解液中 VCAM-1 的表达进行直接 Western 印迹分析.
VCAM-1 抗体 (M/K-2): sc-18864. 在非还原条件下, 对 Sol8 全细胞裂解液中 VCAM-1 的表达进行 Western 印迹分析.

VCAM-1 抗体 (M/K-2): sc-18864

4.3(8)
寫評論提問

说明书
  • VCAM-1 抗体 M/K-2 是大鼠单克隆 IgG1, 在14篇文献中引用，提供 200 µg/ml
  • 针对mouse骨髓基质细胞
  • 抗-VCAM-1 抗体 (M/K-2) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat, human 和porcine 来源的 VCAM-1
  • 抗VCAM-1抗体（M/K-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还提供无叠氮化物阻断剂，sc-18864 L，200 µg/0.1 ml
  • 关于如何获取VCAM-1 (M/K-2): sc-18864的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成VCAM-1 抗体 (M/K-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

VCAM 抗体

VCAM-1抗体（M/K-2）是一种鼠单克隆IgG1抗体，针对小鼠骨髓基质细胞产生，可与小鼠、大鼠、猪和人VCAM-1发生反应。血管细胞粘附分子1（VCAM-1），也称为CD106，是一种属于免疫球蛋白超家族的第一类跨膜糖蛋白。从结构上看，VCAM-1由七个类免疫球蛋白细胞外结构域、一个跨膜区段和一个短的细胞质尾部组成；这种结构至关重要，因为类免疫球蛋白结构域有助于与白细胞上的整合素结合，尤其是VLA-4（α4β1整合素）。 VCAM-1结合介导白细胞与血管内皮细胞的粘附，这是免疫监视和炎症反应的关键步骤。炎症细胞因子（如白细胞介素-1、肿瘤坏死因子-α和脂多糖）可上调内皮细胞上的VCAM-1表达。了解VCAM-1的结构特征非常重要，因为其变化会影响整合素的相互作用，从而影响白细胞的粘附和迁移，而白细胞的粘附和迁移在动脉粥样硬化和自身免疫性疾病等病症中至关重要。抗VCAM-1抗体（M/K-2）适用于包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）在内的多种应用。VCAM-1（M/K-2）单克隆抗体有助于研究内皮细胞活化、白细胞粘附和血管炎症的机理。对VCAM-1的研究有助于制定针对免疫细胞异常迁移和血管功能障碍等疾病的疗法。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

VCAM-1 抗体 (M/K-2)

sc-18864
200 µg/ml
RMB2377.00

VCAM-1 抗体 (M/K-2) L

sc-18864 L
200 µg/0.1 ml
RMB2377.00

VCAM-1 抗体 (M/K-2) AC

sc-18864 AC
500 µg/ml, 25% agarose
RMB3129.00

VCAM-1 抗体 (M/K-2) HRP

sc-18864 HRP
200 µg/ml
RMB2377.00

VCAM-1 抗体 (M/K-2) FITC

sc-18864 FITC
200 µg/ml
RMB2482.00

VCAM-1 抗体 (M/K-2) PE

sc-18864 PE
200 µg/ml
RMB2580.00

VCAM-1 抗体 (M/K-2) Alexa Fluor® 488

sc-18864 AF488
200 µg/ml
RMB2685.00

VCAM-1 抗体 (M/K-2) Alexa Fluor® 546

sc-18864 AF546
200 µg/ml
RMB2685.00

VCAM-1 抗体 (M/K-2) Alexa Fluor® 594

sc-18864 AF594
200 µg/ml
RMB2685.00

VCAM-1 抗体 (M/K-2) Alexa Fluor® 647

sc-18864 AF647
200 µg/ml
RMB2685.00

VCAM-1 抗体 (M/K-2) Alexa Fluor® 680

sc-18864 AF680
200 µg/ml
RMB2685.00

VCAM-1 抗体 (M/K-2) Alexa Fluor® 790

sc-18864 AF790
200 µg/ml
RMB2685.00

Do you have fluorophore conjugated versions of this antibody for flow cytometry? Thanks!

Asked by: Anonymous
Thank you for your question. We offer Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647. For a price quote and ship date please email [email protected].
Answered by: BlakeJ
Date published: 2024-09-20

Hallo, welcher Sekundärantikörper eignet sich denn am besten für den Primärantikörper VCAM-1 (M/K-2): sc-18864? Vielen Dank!

Asked by: VLV22
Vielen Dank für Ihre Frage. Derzeit bieten wir leider keine anti Ratte Sekundärantikörper an.
Answered by: Tech Support Europe
Date published: 2022-07-01

Hi, I noticed the VCAM1 antibodies(VCAM-1 Antibody (M/K-2): sc-18864) are in 0.1% gelatin. Do you have a carrier free version of this antibody? If not, what is the molecular weight of the gelatin? Thanks, Jia

Asked by: Jia Hao
Thank you for your question. We are able to offer this antibody carrier free as a special order. We also offer the L product (sc-18864 L) which is provided in sterile PBS (gelatin and azide-free). The molecular weight of the gelatin is 150-160 kDa. Please contact our Special Orders Department to inquire about availability and to receive a quote. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Technical Support
Date published: 2019-08-20

Can VCAM-1 (M/K-2): sc-18864 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, VCAM-1 (M/K-2): sc-18864 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody!This antibody works extremely well for WB on mouse cells.
Date published: 2019-08-19
Rated 5 out of 5 by from Highly recommended for IHC(P)Produced beautiful cytoplasmic immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue
Date published: 2017-02-08
Rated 4 out of 5 by from Robust band seen in WB using Sol8 whole cellRobust band seen in WB using Sol8 whole cell lysate. -SCBT QC
Date published: 2015-05-05
Rated 4 out of 5 by from Using in WB and IF with excellent resultsUsing in WB and IF with excellent results.
Date published: 2015-04-24
Rated 4 out of 5 by from Publishable data in FCM using human HUVECsPublishable data in FCM using human HUVECs. -SCBT Publication Review
Date published: 2015-04-06
Rated 4 out of 5 by from Publishable data in ICC using human THBMECPublishable data in ICC using human THBMEC cell lines. -SCBT Publication Review
Date published: 2015-03-23
Rated 4 out of 5 by from Produced wonderful immunoperoxidase cytoplasmicProduced wonderful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-02-13
Rated 4 out of 5 by from Publishable data in WB using human HSE cell linesPublishable data in WB using human HSE cell lines. -SCBT Publication Review
Date published: 2015-02-07
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VCAM-1 抗体 (M/K-2) is rated 4.3 out of 5 by 8.
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