Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
VCAM-1 Antibody (E-10) - Immunohistochemistry - Image 5074
VCAM-1 抗体 (E-10): sc-13160. 福尔马林固定, 石蜡包埋的人扁桃体的免疫过氧化物酶染色, 显示膜染色.
VCAM-1 抗体 (E-10): sc-13160. 福尔马林固定, 石蜡包埋的人扁桃体的免疫过氧化物酶染色, 显示膜染色.

VCAM-1 抗体 (E-10): sc-13160

3.9(33)
寫評論提問

说明书
  • VCAM-1 抗体 E-10 是小鼠单克隆 IgG1 κ，VCAM-1抗体, 在201篇文献中引用，规格为200 µg/ml
  • 免疫human物种的VCAM-1的氨基酸25-300
  • VCAM-1 抗体 (E-10) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 VCAM-1
  • 抗VCAM-1抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 关于如何获取VCAM-1 (E-10): sc-13160的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 VCAM-1 抗体 (E-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与VCAM-1 抗体 (E-10) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

VCAM 抗体

VCAM-1抗体（E-10）是一种小鼠单克隆IgG1 kappa轻链抗体，针对人类血管细胞粘附分子1（VCAM-1）的25-300个氨基酸。VCAM-1，也称为CD106，是一种跨膜糖蛋白，属于免疫球蛋白超家族，主要在活化的内皮细胞上表达。从结构上看，VCAM-1由七个免疫球蛋白样结构域组成，这些结构域对于与白细胞上的整合素（如VLA-4（α4β1））结合至关重要，有助于其在免疫反应期间粘附并穿过内皮细胞。N-连接糖基化等翻译后修饰对于VCAM-1的正常折叠、稳定性和功能至关重要，影响其与整合素的相互作用以及整个细胞粘附过程。VCAM-1的表达受白细胞介素-1（IL-1）、肿瘤坏死因子-α（TNF-α）和脂多糖（LPS）等炎症细胞因子的上调，从而将VCAM-1与动脉粥样硬化和类风湿性关节炎等多种炎症性疾病联系起来。抗VCAM-1抗体（E-10）可与小鼠、大鼠和人类VCAM-1发生反应，适用于包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）在内的多种应用。VCAM-1 (E-10) 抗体是研究内皮活化、白细胞粘附和炎症过程机理的重要工具。了解 VCAM-1 的结构和翻译后修饰对于制定针对异常免疫细胞迁移和血管功能障碍疾病的疗法至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VCAM-1 抗体 (E-10) 参考文献:

  1. 肿瘤坏死因子与 IL-4 或 IFN-gamma 结合可选择性地增强内皮细胞对 T 细胞的粘附性。血管细胞粘附分子-1 依赖性和非依赖性结合机制的贡献。  |  Thornhill, MH., et al. 1991. J Immunol. 146: 592-8. PMID: 1702807
  2. 血管细胞粘附分子-1（VCAM-1）--对肿瘤发生和转移中其作用的认识日益深入。  |  Schlesinger, M. and Bendas, G. 2015. Int J Cancer. 136: 2504-14. PMID: 24771582
  3. 血管细胞粘附分子-1（VCAM-1）在免疫疾病和癌症中的新作用。  |  Kong, DH., et al. 2018. Int J Mol Sci. 19: PMID: 29614819
  4. 脂质诱导的内皮血管细胞粘附分子1促进非酒精性脂肪性肝炎发病。  |  Furuta, K., et al. 2021. J Clin Invest. 131: PMID: 33476308
  5. 神经细胞粘附分子（NCAM）作为突触重塑的定量标记。  |  Jørgensen, OS. 1995. Neurochem Res. 20: 533-47. PMID: 7643959
  6. NCAM使用的Vase小外显子并不局限于神经外胚层肿瘤。  |  Patel, K., et al. 1993. Int J Cancer. 54: 772-7. PMID: 8325706
  7. 精神分裂症的卡尔曼综合征变异型（KSV）模型。  |  Cowen, MA. and Green, M. 1993. Schizophr Res. 9: 1-10. PMID: 8461265
  8. 内皮-白细胞粘附分子。  |  Bevilacqua, MP. 1993. Annu Rev Immunol. 11: 767-804. PMID: 8476577
  9. 一名威尔姆斯局部肿瘤患者的骨髓微转移。  |  Dominici, C., et al. 1996. Med Pediatr Oncol. 26: 125-8. PMID: 8531850
  10. 粘膜血管地址素与其淋巴细胞受体α4β7结合的结构要求。整合素-Ig 家族相互作用的共同主题。  |  Briskin, MJ., et al. 1996. J Immunol. 156: 719-26. PMID: 8543825
  11. 蛋白酶 3 抗体介导血管细胞粘附分子-1（VCAM-1）的表达。  |  Mayet, WJ., et al. 1996. Clin Exp Immunol. 103: 259-67. PMID: 8565309
  12. Hox 和 Pax 基因产品对 N-CAM 表达的发育控制。  |  Edelman, GM. and Jones, FS. 1995. Philos Trans R Soc Lond B Biol Sci. 349: 305-12. PMID: 8577842

订购信息

产品名称产品编号规格价格数量收藏夹

VCAM-1 抗体 (E-10)

sc-13160
200 µg/ml
RMB2377.00

VCAM-1 (E-10): m-IgG Fc BP-HRP 套装

sc-528279
200 µg Ab; 10 µg BP
RMB2662.00

VCAM-1 (E-10): m-IgGκ BP-HRP 套装

sc-520618
200 µg Ab, 40 µg BP
RMB2662.00

VCAM-1 (E-10): m-IgG1 BP-HRP 套装

sc-542849
200 µg Ab; 20 µg BP
RMB2662.00

VCAM-1 抗体 (E-10) AC

sc-13160 AC
500 µg/ml, 25% agarose
RMB3129.00

VCAM-1 抗体 (E-10) HRP

sc-13160 HRP
200 µg/ml
RMB2377.00

VCAM-1 抗体 (E-10) FITC

sc-13160 FITC
200 µg/ml
RMB2482.00

VCAM-1 抗体 (E-10) PE

sc-13160 PE
200 µg/ml
RMB2580.00

VCAM-1 抗体 (E-10) Alexa Fluor® 488

sc-13160 AF488
200 µg/ml
RMB2685.00

VCAM-1 抗体 (E-10) Alexa Fluor® 546

sc-13160 AF546
200 µg/ml
RMB2685.00

VCAM-1 抗体 (E-10) Alexa Fluor® 594

sc-13160 AF594
200 µg/ml
RMB2685.00

VCAM-1 抗体 (E-10) Alexa Fluor® 647

sc-13160 AF647
200 µg/ml
RMB2685.00

VCAM-1 抗体 (E-10) Alexa Fluor® 680

sc-13160 AF680
200 µg/ml
RMB2685.00

VCAM-1 抗体 (E-10) Alexa Fluor® 790

sc-13160 AF790
200 µg/ml
RMB2685.00

VCAM-1 抗体 (E-10) Alexa Fluor® 405

sc-13160 AF405
200 µg/ml
RMB2685.00

Can I use this sc-13160 for blocking VCAM experiments?

Asked by: Emily
Thank you for your question. We do not test our antibodies for their blocking capabilities. They are only tested in regards to detection assays.
Answered by: Tech Support Europe
Date published: 2024-03-28

Hello, what animal was it raised in? Thanks

Asked by: brewing
Thank you for your question. VCAM-1 Antibody (E-10) is a mouse monoclonal IgG1 κ VCAM-1 antibody.
Answered by: Tech Support Europe
Date published: 2024-01-09

What is the binding site? I need extracellular domain or N-terminal binding immunogenecity. do youy have any?

Asked by: BRISHTI
Thank you for your question. VCAM-1 (E-10) binds to the extracellular domain.
Answered by: BlakeJ
Date published: 2023-05-31

Hi, I have been trying to do a WB using VCAM antibody sc 13160 on HUVEC protein samples. The blot didn't show a band at 110Kda (but I had lower non specific bands). I have heat up my samples prior loading and used reducing conditions. Can you help please?

Asked by: Flo75
Thank you for your question. Please get in touch with our technical service department so we can assist you with your experiment.
Answered by: Technical Support Europe
Date published: 2020-10-12

Can VCAM-1(E-10): sc-13160 monoclonal antibody be used to stain formalin-fixed, paraffin embedded tissue sections? What antigen retrieval buffer do you recommend?

Asked by: tomerines
Thank you for your question. Yes, VCAM-1 Antibody (E-10): sc-13160, can be used to stain formalin-fixed paraffin-embedded tissue sections. This antibody is recommended for IHC(P) in our recommended applications. We recommend using sodium citrate buffer (pH 6.0) plus heat treatment for antigen retrieval. A link to our recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-11-08

Vorrei saper quali sono le cellule da poter utilizzare come controllo di questo anticorpo

Asked by: Federica
Grazie mille per la domanda, due possibili lisati utilizzabili come controllo sono Control 293T Lysate: sc-117752 e VCAM-1 (h): Lisato 293T: sc-178116. Per favore contatti il nostro servizio tecnico via telefono, email o live chat per ulteriori informazioni
Answered by: Technical Support Europe
Date published: 2017-09-15

What secondary is best to use with this antibody?

Asked by: AbPolly
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with our VCAM-1 sc-13160, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 12
Date published: 2017-01-04
  • y_2025, m_9, d_3, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_7
  • loc_zh_CN, sid_13160, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 105ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Bad work in WB against Human cellsI tried this antibody in HUVEC treated or not with TNF-alpha and it did not work or very bad work, even at 1:1000 or 1:500 dilution.
Date published: 2024-03-29
Rated 5 out of 5 by from works greatThis antibody works excellent in western blot. we highly recommend for other users for similar purposes.
Date published: 2024-01-30
Rated 1 out of 5 by from Did not work for me in WBI tried this antibody in HUVECs cells at 1:1000 dilution but it did not work. I will try at 1:500
Date published: 2023-03-08
Rated 5 out of 5 by from This antibody works well in HUVEC.In Western blot using TNF-alpha treated HUVEC cell lysate, this antibody worked well. However, non-specific binding was observed in mouse-derived cells. So I recommend this product to detect human origin VCAM-1.
Date published: 2022-10-13
Rated 1 out of 5 by from Did not work for me in FCM applicationI bought this antibody because I needed to see the increase of VCAM-1 expression in TNFalpha activated HUVEC cells. However, though I tested the antibody at several concentrations (starting at 1.50 to 1.1000), and different secondary antibodies, I did not see any signal. The cells were correctly activated as I could see a huge increase in ICAM-1 membrane expression.
Date published: 2022-01-06
Rated 2 out of 5 by from western blotsc-13160を用いて、western blotを検討しましたが、特異的なバンドを検出することは出来ませんでした。
Date published: 2021-04-01
Rated 5 out of 5 by from Great Specific VCAM1 Antibody.I bought this antibody after using a VCAM1 antibody from another company that was very hit and miss. This antibody worked fantastically at the recommended concentration and was sensitive. I found that western blotting for VCAM-1 requires reducing conditions, so those who are finding multiple bands or poor resolution may wish to try this. The picture attached shows VCAM-1 in response to increasing TNFa concentrations in Human Microvascular Endothelial cell lysates. I run far too much purified VCAM1 in the first lane, hence the signal below.
Date published: 2020-08-27
Rated 5 out of 5 by from Very good antibodyWe bought and used this antibody in WB on total lysate from human endothelial cells EA.hy926
Date published: 2019-01-31
  • y_2025, m_9, d_3, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_33
  • loc_zh_CN, sid_13160, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
VCAM-1 抗体 (E-10) is rated 3.9 out of 5 by 33.
  • y_2025, m_9, d_3, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_33
  • loc_zh_CN, sid_13160, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 104ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。