Date published: 2025-9-8

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Vasa 抗体 (C-2): sc-514249

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说明书
  • Vasa抗体(C-2)是小鼠单克隆IgM (kappa轻链),规格为200 µg/ml
  • 特异性抗原位于Drosophila melanogaster物种的Vasa的N-terminus的氨基酸4-21之间
  • 推荐用于 d 来源的Vasa WB, IP, IF 和 ELISA检测
  • 另提供琼脂糖偶联抗体,规格为琼脂糖装,可用于IP实验;HRP 偶联抗体用于WB, IHC(P)和ELISA实验;以及藻红蛋白、FITC、Alexa Fluor® 488Alexa Fluor® 647 偶联抗体,规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取Vasa (C-2): sc-514249免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Vasa 抗体 (C-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    Vasa抗体(C-2)是一种IgM κ小鼠单克隆Vasa抗体,可通过WB、IP、IF和ELISA检测果蝇来源的Vasa。Vasa抗体(C-2)既有偶联型也有非偶联型抗Vasa抗体形式。VASA是一种含有724个氨基酸的ATP依赖性RNA解旋酶,属于DEAD-box家族。VASA在整个生命周期中特异性表达于生殖细胞,在体细胞中无法检测到。在脊椎动物中,VASA仅限于两性生殖生物。它存在于细胞质中,仅存在于性腺脊区域的迁移性原始生殖细胞中。在睾丸切片上,VASA在精原细胞中的表达最高,在精母细胞中减少,在精子细胞中表达较低,在精子中不表达。在卵巢中,VASA在卵原细胞中的表达最高,但在整个卵子发生过程中持续表达。VASA的N端富含甘氨酸,并含有多个重复的RGG基序,这些基序被认为具有RNA结合功能。具体来说,它调节对分化至关重要的复杂mRNAs的翻译。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Vasa 抗体 (C-2) 参考文献:

    1. 人类 VASA 基因在生殖细胞系中特异性表达。  |  Castrillon, DH., et al. 2000. Proc Natl Acad Sci U S A. 97: 9585-90. PMID: 10920202
    2. 成年牛组织, 卵母细胞和植入前胚胎中生殖细胞标记基因 MATER, ZAR1, GDF9, BMP15 和 VASA 的时空表达。  |  Pennetier, S., et al. 2004. Biol Reprod. 71: 1359-66. PMID: 15189828
    3. 胎儿生殖细胞表达睾丸原位癌标记物的病理生物学意义。  |  Honecker, F., et al. 2004. J Pathol. 203: 849-57. PMID: 15221945
    4. 人类雌性生殖细胞在产前性腺发育过程中的分化和发育:一项免疫组织化学研究。  |  Stoop, H., et al. 2005. Hum Reprod. 20: 1466-76. PMID: 15734757
    5. 双性繁殖和雌性繁殖脊椎动物鲫鱼(Carassius auratus gibelio)精子形成和卵子形成过程中Vasa RNA和蛋白质的差异表达。  |  Xu, H., et al. 2005. Dev Dyn. 233: 872-82. PMID: 15880437
    6. RNA 螺旋酶:分化的调节器。  |  Abdelhaleem, M. 2005. Clin Biochem. 38: 499-503. PMID: 15885226
    7. 弯曲变形:DEAD-box 螺旋酶 Vasa 的 RNA 解旋。  |  Linder, P. and Lasko, P. 2006. Cell. 125: 219-21. PMID: 16630807
    8. 太平洋南美白对虾类血管基因的特征及其在卵子发生过程中的表达。  |  Aflalo, ED., et al. 2007. Mol Reprod Dev. 74: 172-7. PMID: 16955407

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Vasa 抗体 (C-2)

    sc-514249
    200 µg/ml
    RMB2377.00

    Vasa 抗体 (C-2) AC

    sc-514249 AC
    500 µg/ml, 25% agarose
    RMB3129.00

    Vasa 抗体 (C-2) Alexa Fluor® 488

    sc-514249 AF488
    200 µg/ml
    RMB2685.00

    Vasa 抗体 (C-2) Alexa Fluor® 647

    sc-514249 AF647
    200 µg/ml
    RMB2685.00

    Vasa 抗体 (C-2) FITC

    sc-514249 FITC
    200 µg/ml
    RMB2482.00

    Vasa 抗体 (C-2) HRP

    sc-514249 HRP
    200 µg/ml
    RMB2377.00

    Vasa (C-2) 中和勝肽

    sc-514249 P
    100 µg/0.5 ml
    RMB511.00

    Vasa 抗体 (C-2) PE

    sc-514249 PE
    200 µg/ml
    RMB2580.00

    From where can I get the peptide sequence of this antibody?

    Asked by: bobs
    Thank you for your question.specific for an epitope mapping between amino acids 4-21 at the N-terminus of Vasa of Drosophila melanogaster origin.You can check it in uniprot. Thank you. https://www.uniprot.org/uniprotkb/P09052/entry#sequences
    Answered by: Jiajun Z
    Date published: 2024-10-14

    Could I ask free sample for trying ?

    Asked by: ChunMing Lai
    Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
    Answered by: Tech Service
    Date published: 2019-05-31

    What application is the blocking peptide sc-514249 P appropriate for?

    Asked by: AbPolly
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-24
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    Rated 1 out of 5 by from Did not work in IFFailed to yield IF signal in Drosophila testis and ovary.
    Date published: 2018-04-02
    Rated 5 out of 5 by from It' useful.It gives excellent WB signal. No issues.It is suggested that the others to use.
    Date published: 2016-12-08
    Rated 5 out of 5 by from Great Western blot data of Vasa expressionGreat Western blot data of Vasa expression in Schneider s Drosophila Line 2 whole cell lysate. -SCBT QC
    Date published: 2013-06-13
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    Vasa 抗体 (C-2) is rated 3.7 out of 5 by 3.
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