Date published: 2025-9-7
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V-ATPase D Antibody (D-4) - Western Blotting - Image 374700
V-ATPase D 抗体 (D-4): sc-166218. SH-SY5Y (A) 和 C6 (B) 全细胞裂解液及小鼠脑组织提取物 (C) 中 V-ATPase D 表达的 Western 印迹分析.
V-ATPase D 抗体 (D-4): sc-166218. SH-SY5Y (A) 和 C6 (B) 全细胞裂解液及小鼠脑组织提取物 (C) 中 V-ATPase D 表达的 Western 印迹分析.

V-ATPase D 抗体 (D-4): sc-166218

4.8(4)
寫評論提問

说明书
  • V-ATPase D抗体(D-4)是小鼠单克隆IgG1 κ, 在8篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长V-ATPase D的氨基酸1-243
  • 推荐用于 mouse, rat 和 human 来源的V-ATPase D WB, IP, IF 和 ELISA检测
  • 1 BP-HRP">m-IgG1 BP-HRP是V-ATPase D Antibody (D-4) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与V-ATPase D Antibody (D-4) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

附加V-ATPase抗体，包括V-ATPase A1, V-ATPase α 1, V-ATPase B1, V-ATPase B2, V-ATPase C1, V-ATPase C2, V-ATPase D, V-ATPase D1, V-ATPase D2, V-ATPase G1, V-ATPase G2和V-ATPase H

液泡型H+-ATP酶（V-ATPase）是一种负责真核细胞内细胞器酸化的多亚基酶。V-ATPases逆电化学梯度泵送质子，而F-ATPases则逆转这一过程，从而合成ATP。负责ATP水解的外围V1域和负责质子转位的整体V0域组成了V-ATPase。九个亚基（A-H）组成了V1域，五个亚基（a、d、c、c'和c"）组成了V0域。与F-ATPase一样，V-ATPase最有可能通过旋转机制进行操作。V-ATPase C是一种普遍表达的辅助亚基。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

V-ATPase D 抗体 (D-4) 参考文献:

  1. 小鼠V-ATP酶d亚基异构体的表达和功能。  |  Nishi, T., et al. 2003. J Biol Chem. 278: 46396-402. PMID: 12963731
  2. 巨噬细胞特异性V-ATP酶亚基ATP6V0D2通过促进自噬体-溶酶体融合来限制炎性体的激活和细菌感染。  |  Xia, Y., et al. 2019. Autophagy. 15: 960-975. PMID: 30681394
  3. 人类阿尔茨海默氏症大脑中磷酸化tau蛋白的相互作用。  |  Drummond, E., et al. 2020. Brain. 143: 2803-2817. PMID: 32812023
  4. 五味子醇 A 通过异构靶细胞 V-ATPase 的 ATP6V0d1 亚基保护 AGE 诱导的神经细胞死亡。  |  Zhou, X., et al. 2022. Acta Pharm Sin B. 12: 3843-3860. PMID: 36213534
  5. ATP6V0D1 通过阻断 STAT3 介导的溶酶体 pH 平衡促进碱中毒。  |  Chen, F., et al. 2023. Cell Rep. 42: 111911. PMID: 36640329
  6. 一种新型体细胞突变涉及ATP6V0D1在胰岛素前体加工中的作用。  |  Avari, P., et al. 2023. J Endocr Soc. 7: bvac196. PMID: 36694809
  7. 碱中毒的机制。  |  Chen, F., et al. 2023. Front Cell Dev Biol. 11: 1213995. PMID: 37601110
  8. 肌动蛋白通过增加IRS-1的表达逆转胰岛素抵抗并改善心肌病，该作用在小鼠脂肪缺乏引起的脂质营养不良模型中由溶酶体H+-ATP酶V0d1亚基引起。  |  Yuan, W., et al. 2024. Theranostics. 14: 2246-2264. PMID: 38505620
  9. 碱性磷酸酶诱导通过ATP6V0D1介导的ABCB1抑制来对抗紫杉醇耐药性卵巢癌细胞。  |  Chen, F., et al. 2024. Mol Carcinog. 63: 1515-1527. PMID: 38751020
  10. 体育锻炼通过抑制阿尔茨海默病中的小胶质细胞Tmem9-ATP6V0D1，减少补体介导的突触丢失并防止认知障碍。  |  Li, S., et al. 2025. Aging Cell. e14496. PMID: 39871402

订购信息

产品名称产品编号规格价格数量收藏夹

V-ATPase D 抗体 (D-4)

sc-166218
200 µg/ml
RMB2377.00

V-ATPase D (D-4): m-IgG1 BP-HRP 套装

sc-542659
200 µg Ab; 20 µg BP
RMB2662.00

What is the recommended fixation method for immunofluorescence staining with V-ATPase D (D-4): sc-166218 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Positive detection of endogenous proteinPositive detection of endogenous protein expression levels of the V-ATPase D protein in mouse and rat brain tissue extracts and SK-N-SH whole cell lysate. -SCBT QC
Date published: 2015-05-11
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from Publishable results obtained by WB usingPublishable results obtained by WB using human Huh7 cells. -SCBT Publication Review
Date published: 2015-01-29
Rated 5 out of 5 by from Product scProduct sc-166218 has been reported to be suitable for use in WB using mouse samples. -SCBT Publication Review
Date published: 2015-01-16
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V-ATPase D 抗体 (D-4) is rated 4.8 out of 5 by 4.
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