Date published: 2025-9-7
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uPA/urokinase/PLAU 抗体 (H77A10): sc-59727

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说明书
  • uPA/urokinase/PLAU 抗体 H77A10 是小鼠单克隆 IgG1 κ，uPA/urokinase/PLAU抗体, 在15篇文献中引用，规格为200 µg/ml
  • 针对与mouse来源的全长野生型（糖基化）uPA对应的重组蛋白
  • uPA/urokinase/PLAU 抗体 (H77A10) 推荐用于 WB, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 uPA
  • 抗uPA/urokinase/PLAU抗体（H77A10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取uPA/urokinase/PLAU (H77A10): sc-59727的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 uPA/urokinase/PLAU 抗体 (H77A10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与uPA/urokinase/PLAU 抗体 (H77A10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

uPA抗体（H77A10）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）在小鼠、大鼠和人类样本中特异性检测尿激酶型纤溶酶原激活剂（uPA），也称为PLAU或尿激酶。u PA单克隆抗体（H77A10）是针对一种重组蛋白制备的，该重组蛋白与全长、糖基化的小鼠uPA相对应。uPA是一种丝氨酸蛋白酶，对于将纤溶酶原转化为纤溶酶至关重要，在纤维蛋白溶解和细胞外基质降解中发挥着重要作用。uPA主要位于细胞表面，与其受体uPAR结合，后者定位其蛋白水解活性并促进细胞迁移和组织重塑等过程。uPA在细胞表面的定位使其能够进行定向蛋白水解，这对于伤口愈合、炎症和肿瘤转移至关重要。通过与uPAR相互作用，uPA能够影响细胞信号通路，从而调节细胞的粘附、增殖和存活。uPA的活性受到丝氨酸蛋白酶抑制剂PAI-1和PAI-2的严格调控，这两种抑制剂能够抑制uPA并控制其在生理和病理过程中的作用。抗uPA抗体（H77A10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，为不同的实验应用提供了多功能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

uPA/urokinase/PLAU 抗体 (H77A10) 参考文献:

  1. 组织纤溶酶原激活剂与人类内皮细胞的结合。B 链作为配体的重要性。  |  Cheng, XF., et al. 1992. Biochem J. 287 (Pt 2): 407-13. PMID: 1332673
  2. 人类尿激酶-肌钙蛋白原激活剂基因及其启动子。  |  Riccio, A., et al. 1985. Nucleic Acids Res. 13: 2759-71. PMID: 2987867
  3. 人类组织纤溶酶原激活剂基因。  |  Degen, SJ., et al. 1986. J Biol Chem. 261: 6972-85. PMID: 3009482
  4. 组织型纤溶酶原激活剂：一种新的纤维蛋白溶解剂。  |  Milligan, KS. 1987. Heart Lung. 16: 69-74. PMID: 3098703
  5. 组织纤溶酶原激活剂。  |  Loscalzo, J. and Braunwald, E. 1988. N Engl J Med. 319: 925-31. PMID: 3138537
  6. 器官型细胞培养系统中角质形成细胞分化过程中尿激酶型纤溶酶原激活剂（uPA）, 其受体（uPA-R）和 2 型抑制剂（PAI-2）的差异表达。  |  Schaefer, BM., et al. 1995. Exp Cell Res. 220: 415-23. PMID: 7556451
  7. 纤维蛋白溶解对安可瑞疗法的反应：纤维蛋白原和纤维蛋白降解产物的特征。  |  Prentice, CR., et al. 1993. Br J Haematol. 83: 276-81. PMID: 8457476

订购信息

产品名称产品编号规格价格数量收藏夹

uPA/urokinase/PLAU 抗体 (H77A10)

sc-59727
200 µg/ml
RMB2377.00

uPA/urokinase/PLAU (H77A10): m-IgG Fc BP-HRP 套装

sc-526940
200 µg Ab; 10 µg BP
RMB2662.00

uPA/urokinase/PLAU (H77A10): m-IgG1 BP-HRP 套装

sc-532313
200 µg Ab; 20 µg BP
RMB2662.00

uPA/urokinase/PLAU 抗体 (H77A10) AC

sc-59727 AC
500 µg/ml, 25% agarose
RMB3129.00

uPA/urokinase/PLAU 抗体 (H77A10) HRP

sc-59727 HRP
200 µg/ml
RMB2377.00

uPA/urokinase/PLAU 抗体 (H77A10) FITC

sc-59727 FITC
200 µg/ml
RMB2482.00

uPA/urokinase/PLAU 抗体 (H77A10) PE

sc-59727 PE
200 µg/ml
RMB2580.00

uPA/urokinase/PLAU 抗体 (H77A10) Alexa Fluor® 488

sc-59727 AF488
200 µg/ml
RMB2685.00

uPA/urokinase/PLAU 抗体 (H77A10) Alexa Fluor® 546

sc-59727 AF546
200 µg/ml
RMB2685.00

uPA/urokinase/PLAU 抗体 (H77A10) Alexa Fluor® 594

sc-59727 AF594
200 µg/ml
RMB2685.00

uPA/urokinase/PLAU 抗体 (H77A10) Alexa Fluor® 647

sc-59727 AF647
200 µg/ml
RMB2685.00

uPA/urokinase/PLAU 抗体 (H77A10) Alexa Fluor® 680

sc-59727 AF680
200 µg/ml
RMB2685.00

uPA/urokinase/PLAU 抗体 (H77A10) Alexa Fluor® 790

sc-59727 AF790
200 µg/ml
RMB2685.00

Are there any issues with using sc-59727: uPA (H77A10) mouse monoclonal primary antibody on mouse samples?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-59727 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from Great for WBIt is a good antibody in WB. It works in dilution 1:1000.
Date published: 2024-03-20
Rated 4 out of 5 by from works quite wellI have used a trial sample of this antibody dilution 1:500 (5% milk in TBST - Tween20 - 0,1%), incubation overnight, which gave a clear signal
Date published: 2021-09-24
Rated 5 out of 5 by from Great product!!It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from great antibodyI ordered this product month ago and used it for IF and Western blotting in cultured cells and skin tissues. It works very well for both in term of its specificity and sensitivity.
Date published: 2018-01-02
Rated 5 out of 5 by from The use of sc-59727(uPA) antibodyThree weeks ago I purchased the antibody test equipment, the specificity of the antibody is excellent. WB strip is very clear, and our research group has purchased the antibody.
Date published: 2017-09-13
Rated 5 out of 5 by from good quanlities!WB on HCC cells, dilute in 1:500, show specific and clearly bands.
Date published: 2017-05-03
Rated 1 out of 5 by from No bands seenWestern blotting was performed using twelve different mouse osteosarcoma cell lines which were previously confirmed to express uPA gene based on RT-PCR. Conditions: 12% gel electrophoresis, wet transfer over 2 hours, primary antibodies at 1:200 dilution in 5% mild blocking solution (4C overnight). There was no band visible following the protocol, although positive control (b-actin) was positive.
Date published: 2017-03-08
Rated 5 out of 5 by from perfect resultI have used this antibody to detect this protein in mouse lung tumors. It works well in IHC and western blot.
Date published: 2017-01-06
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uPA/urokinase/PLAU 抗体 (H77A10) is rated 4.2 out of 5 by 9.
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