Date published: 2025-9-8

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UCK1 抗体 (E-9): sc-373940

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说明书
  • UCK1 抗体 E-9 是小鼠单克隆 IgM (kappa轻链),UCK1抗体,规格为200 µg/ml
  • 特异性抗原位于mouse物种的UCK1的N-terminus附近的氨基酸1-27之间
  • 推荐用于 mouse, rat 和 human 来源的UCK1 WB, IP, IF 和 ELISA检测
  • 关于如何获取UCK1 (E-9): sc-373940免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成UCK1 抗体 (E-9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    UCK1抗体(E-9)是一种IgM κ小鼠单克隆UCK1抗体(也称为UCK1抗体),它通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的UCK1蛋白。UCK1抗体(E-9)可作为非偶联抗UCK1抗体。尿苷-胞苷激酶(UCK)在核苷类似物的磷酸化中起重要作用,这可能对癌症的化疗很重要。UCK家族由UCK1和UCK2两个成员组成,它们都在许多肿瘤细胞中表达。UCK1(尿苷-胞苷激酶1),也称为URK1、尿苷单磷酸激酶1或胞苷单磷酸激酶1,是一种由277个氨基酸组成的蛋白质,在骨骼肌、心脏、肝脏和肾脏中表达。UCK1利用ATP或GTP催化尿苷和胞苷分别磷酸化为尿苷单磷酸和胞苷单磷酸。人类UCK1与其小鼠同源物具有92%的氨基酸同一性,表明其在物种间具有保守的作用。UCK1以两种可选剪接的异构体形式存在,这些异构体由映射到人类9号染色体的基因编码。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    UCK1 抗体 (E-9) 参考文献:

    1. 两种人类尿苷-胞苷激酶对尿苷和胞苷核苷类似物的磷酸化作用。  |  Van Rompay, AR., et al. 2001. Mol Pharmacol. 59: 1181-6. PMID: 11306702
    2. 人类癌细胞对新型抗癌核糖核苷 TAS-106 的敏感性与尿苷-胞苷激酶 2 的表达有关。  |  Shimamoto, Y., et al. 2002. Jpn J Cancer Res. 93: 825-33. PMID: 12149149
    3. 人类尿苷-胞苷激酶 2 的结晶和初步 X 射线分析。  |  Suzuki, NN., et al. 2003. Acta Crystallogr D Biol Crystallogr. 59: 1477-8. PMID: 12876357
    4. 人类尿苷-胞苷激酶特异性, 催化和调控的结构基础。  |  Suzuki, NN., et al. 2004. Structure. 12: 751-64. PMID: 15130468
    5. 尿苷/胞苷激酶 2 在 3'-ethynyl 核苷的抗肿瘤活性中发挥着关键作用。  |  Murata, D., et al. 2004. Drug Metab Dispos. 32: 1178-82. PMID: 15280220
    6. 冬菇发育调节因子 PRIB 的靶基因  |  Miyazaki, Y., et al. 2004. Biosci Biotechnol Biochem. 68: 1898-905. PMID: 15388965
    7. 用体细胞和辐射杂化体面板将人类 1 号染色体上的 UCK2, ATF3 和 RGS18 分配到猪的 4, 9 和 10 号染色体上。  |  Aldenhoven, J., et al. 2006. Cytogenet Genome Res. 112: 341F. PMID: 16484797
    8. 基枝菌 Lentinus edodes 基因 uck1 的克隆, 序列分析和表达,该基因编码 UMP-CMP 激酶,是酿酒酵母 URA6 基因的同源物。  |  Kaneko, S., et al. 1998. Gene. 211: 259-66. PMID: 9602145

    订购信息

    产品名称产品编号规格价格数量收藏夹

    UCK1 抗体 (E-9)

    sc-373940
    200 µg/ml
    RMB2377.00

    UCK1 (E-9) 中和勝肽

    sc-373940 P
    100 µg/0.5 ml
    RMB511.00

    I've been using this anti-body (1:200) to detect UCK1 via WB, but the bands don't appear at the right MW. I'm using mouse liver and adipose tissue. The bands consistently appear next my marker indicating 26kd. Is there some explanation?

    Asked by: J321
    Thank you for your question. For any help with optimizing your results or troubleshooting questions please contact our Technical Service department.
    Answered by: Technical Service 3
    Date published: 2020-11-25

    What application is the blocking peptide sc-373940 P appropriate for?

    Asked by: TinTin
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from Produced exceptional immunofluorescence cytoplasmicProduced exceptional immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
    Date published: 2015-02-04
    Rated 5 out of 5 by from Satisfactory Western Blot data of UCK1 expressionSatisfactory Western Blot data of UCK1 expression in non-transfected and mouse UCK1 transfected 293T whole cell lysates. -SCBT QC
    Date published: 2014-07-02
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    UCK1 抗体 (E-9) is rated 5.0 out of 5 by 2.
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