Date published: 2026-10-20

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Ubiquitin Antibody (F-11) - Western Blotting - Image 354143
泛素抗体 (F-11): sc-271289. JAR (A), Jurkat (B), HeLa (C), MCF7 (D), KNRK (E) 和 A549 (F) 全细胞裂解液中泛素表达的 Western 印迹分析.
泛素抗体 (F-11): sc-271289. JAR (A), Jurkat (B), HeLa (C), MCF7 (D), KNRK (E) 和 A549 (F) 全细胞裂解液中泛素表达的 Western 印迹分析.

Ubiquitin 抗体 (F-11): sc-271289

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寫評論提問
说明书
  • Ubiquitin抗体(F-11)是小鼠单克隆IgG1 κ, 在72篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长Ubiquitin的氨基酸1-76
  • Ubiquitin 抗体 (F-11) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 avian来源的Ubiquitin and polyubiquitin; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗Ubiquitin抗体（F-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP是Ubiquitin Antibody (F-11) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与Ubiquitin Antibody (F-11) 搭配使用（请参阅下面的订购信息）。
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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Ubiquitin抗体，包括Ub, Ub+1和USPL1

泛素抗体（F-11）是一种IgG1 κ型小鼠单克隆泛素抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC(P)）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠、人类和鸟类来源的泛素和多聚泛素。泛素抗体（F-11）有非偶联形式的抗泛素抗体，以及多种偶联形式的抗泛素抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。泛素（Ub）是已知在进化上最为保守的蛋白质之一。泛素的主要功能是通过将异常、外来和错误折叠的蛋白质靶向26S蛋白酶体进行降解，从而清除这些蛋白质。这种由76个氨基酸组成的小蛋白质可以通过泛素的羧基末端基团与受体蛋白质上的赖氨酸氨基基团之间的异肽键共价连接到细胞蛋白质上。为了进行蛋白质水解，必须组装泛素寡聚体。在蛋白质水解底物上常见的泛素链具有一个异肽桥，该桥位于一个泛素分子的赖氨酸48和相邻泛素分子的羧基末端之间。泛素还通过消除抑制性蛋白质（如IκB-α和p27）来调节信号转导级联反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ubiquitin 抗体 (F-11) 参考文献:

  1. 家族性帕金森病基因产物 parkin 是一种泛素蛋白连接酶。  |  Shimura, H., et al. 2000. Nat Genet. 25: 302-5. PMID: 10888878
  2. X-连锁凋亡抑制蛋白的泛素蛋白连接酶活性可促进蛋白酶体降解 Caspase-3，并增强其在 Fas 诱导的细胞死亡中的抗凋亡作用。  |  Suzuki, Y., et al. 2001. Proc Natl Acad Sci U S A. 98: 8662-7. PMID: 11447297
  3. Hdm2的泛素蛋白连接酶活性受核酸抑制。  |  Linares, LK. and Scheffner, M. 2003. FEBS Lett. 554: 73-6. PMID: 14596917
  4. 肌肉萎缩中的泛素蛋白连接酶。  |  Cao, PR., et al. 2005. Int J Biochem Cell Biol. 37: 2088-97. PMID: 16125112
  5. 干扰素诱导的泛素蛋白异肽连接酶（E3）EFP 也是 ISG15 E3 连接酶。  |  Zou, W. and Zhang, DE. 2006. J Biol Chem. 281: 3989-94. PMID: 16352599
  6. 典型和变异拟南芥U-box E3泛素蛋白连接酶的生化功能。  |  Wiborg, J., et al. 2008. Biochem J. 413: 447-57. PMID: 18393940
  7. 指环状泛素蛋白连接酶及其对人类疾病发病机制的影响。  |  Ardley, HC. 2009. Curr Pharm Des. 15: 3697-715. PMID: 19925421
  8. HECT泛素蛋白连接酶E6AP和HERC2的物理和功能相互作用。  |  Kühnle, S., et al. 2011. J Biol Chem. 286: 19410-6. PMID: 21493713
  9. E3 泛素蛋白连接酶 ITCH 的磷酸化会减少与其同源 E2 泛素连接酶的结合。  |  Perez, JM., et al. 2018. J Biol Chem. 293: 1100-1105. PMID: 29212706
  10. 在环核苷酸门控通道缺陷中，通过过表达E3泛素蛋白连接酶synoviolin 1促进内质网逆向转运，从而减轻内质网应激并保护锥体感光细胞。  |  Yang, F., et al. 2024. FASEB J. 38: e70021. PMID: 39215566

订购信息

产品名称产品编号规格价格数量收藏夹

Ubiquitin 抗体 (F-11)

sc-271289
200 µg/ml
CNY2422.00

Ubiquitin (F-11): m-IgG Fc BP-HRP 套装

sc-525795
200 µg Ab; 10 µg BP
CNY2715.00

Ubiquitin 抗体 (F-11) AC

sc-271289 AC
500 µg/ml, 25% agarose
CNY3189.00

Ubiquitin 抗体 (F-11) HRP

sc-271289 HRP
200 µg/ml
CNY2422.00

Ubiquitin 抗体 (F-11) FITC

sc-271289 FITC
200 µg/ml
CNY2527.00

Ubiquitin 抗体 (F-11) PE

sc-271289 PE
200 µg/ml
CNY2625.00

Ubiquitin 抗体 (F-11) Alexa Fluor® 488

sc-271289 AF488
200 µg/ml
CNY2738.00

Ubiquitin 抗体 (F-11) Alexa Fluor® 546

sc-271289 AF546
200 µg/ml
CNY2738.00

Ubiquitin 抗体 (F-11) Alexa Fluor® 594

sc-271289 AF594
200 µg/ml
CNY2738.00

Ubiquitin 抗体 (F-11) Alexa Fluor® 647

sc-271289 AF647
200 µg/ml
CNY2738.00

Ubiquitin 抗体 (F-11) Alexa Fluor® 680

sc-271289 AF680
200 µg/ml
CNY2738.00

Ubiquitin 抗体 (F-11) Alexa Fluor® 790

sc-271289 AF790
200 µg/ml
CNY2738.00

What is the epitope of UB recognized by this antibody?

Asked by: SamD
Thank you for your question. This mouse monoclonal antibody is raised against amino acids 1-76 representing full length Ub of human origin. The exact epitope recognized by this monoclonal antibody has not been determined.
Answered by: Technical Support
Date published: 2019-01-17

Is Ub Antibody (F-11): sc-271289 available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using Ub (P4D1), which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/ub-antibody-p4d1)
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 3 out of 5 by Mjrgc from OK to useModerate signal in murine primary cells but not clear
Date published: 2018-02-03
Rated 5 out of 5 by Klab from Works for western blot of C2C12 proteinWe tried this in western blots and got a clear, double band around 10 kDa. No additional bands were detected up to 50 kDa; higher molecular weights were not tested with this antibody.
Date published: 2017-12-05
Rated 5 out of 5 by huren from good staining antibodyThis antibody gave beautiful immunoperoxidase staining in frozen human normal human duodenum tissue
Date published: 2017-06-03
Rated 5 out of 5 by Setlla from That's what we needThe major component, ubiquitin, is one of the most well-known and most conserved proteins of the phylogeny, and we know that its main function is to target them to remove the abnormalities through the 26S proteosome degradation. That's what we need
Date published: 2017-02-09
Rated 5 out of 5 by Bill28 from Superb product !!One of several high quality Ub monoclonals from scbt; better than polyclonals
Date published: 2017-01-10
Rated 5 out of 5 by DefinitelyNotMatt from Reliable and Versatile AntibodyThis antibody works well with almost every species out there, and produces very strong signal in WB and ELISA assays.
Date published: 2016-12-21
Rated 2 out of 5 by kjkl from not very satisfiedI bought Ub antibody (F-11) few months ago. I have use this antibody only in WB and i am not very satisfied because a lot of background appears making very difficult to distinguish the Ubiquitination of a protein.
Date published: 2016-12-20
Rated 5 out of 5 by JL from Used human and mouse cell lysatesUsed human and mouse cell lysates, detected endogenous Ub in WB -SCBT Publication Review
Date published: 2015-04-23
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Ubiquitin 抗体 (F-11) is rated 4.5 out of 5 by 10.
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