Date published: 2025-9-8
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Ubiquitin Antibody (P4D1) - Immunohistochemistry - Image 356766
泛素抗体 (P4D1) HRP: sc-8017 HRP. 福尔马林固定, 石蜡包埋的人十二指肠组织的直接免疫过氧化物酶染色, 显示腺细胞的细胞核和细胞质染色.
泛素抗体 (P4D1) HRP: sc-8017 HRP. 福尔马林固定, 石蜡包埋的人十二指肠组织的直接免疫过氧化物酶染色, 显示腺细胞的细胞核和细胞质染色.

Ubiquitin 抗体 (P4D1): sc-8017

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说明书
  • Ubiquitin 抗体 P4D1 是小鼠单克隆 IgG1 κ，Ubiquitin抗体, 在3,433篇文献中引用，规格为200 µg/ml
  • 免疫1-76氨基酸，对应于bovine来源Ubiquitin全长
  • Ubiquitin 抗体 (P4D1) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA，检测 mouse, rat, human 和 Drosophila 来源的Ubiquitin, poly-ubiquitinated and ubiquitinated proteins
  • 泛素抗体（P4D1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与phycoerythrin或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素可用于WB、IHC(P)和ELISA实验；以及Alexa Fluor® 405 用于IF、IHC(P)和FCM实验
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 关于如何获取Ubiquitin (P4D1): sc-8017的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Ubiquitin 抗体 (P4D1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ubiquitin 抗体 (P4D1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
说明书与实验方案
研究信息

関連項目

附加Ubiquitin抗体，包括Ub, Ub+1和USPL1

泛素抗体（P4D1）是一种小鼠单克隆IgG1 kappa轻链抗体，用于检测全长泛素蛋白，包括牛源性氨基酸1-76。泛素小鼠单克隆抗体（P4D1）可识别小鼠、大鼠、人类和果蝇来源的泛素，适用于包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）在内的各种应用。抗泛素抗体（P4D1）有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。泛素蛋白本身是进化过程中最保守的蛋白质之一，通过26S蛋白酶体降解异常、外来和折叠不当的蛋白质，从而在细胞稳态中发挥关键作用。泛素通过其羧基末端甘氨酸与目标蛋白上的赖氨酸残基之间的异肽键与底物共价结合，从而实现其功能，促进泛素链的形成，特别是通过赖氨酸48连接，发出蛋白水解的信号。除了蛋白质降解，泛素还通过多种多泛素连接调节信号转导通路，包括赖氨酸63连接，这对于DNA修复和细胞内信号传导等非蛋白水解作用至关重要。泛素调节调节蛋白（如IκB-α和p27）的稳定性，从而影响炎症、细胞周期进展和DNA损伤反应等过程。泛素单克隆抗体（P4D1）推荐用于研究蛋白质周转、细胞信号传导和细胞调节各个方面的研究人员。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ubiquitin 抗体 (P4D1) 参考文献:

  1. 泛素, 蛋白酶体和细胞内蛋白质降解的调控。  |  Hochstrasser, M. 1995. Curr Opin Cell Biol. 7: 215-23. PMID: 7612274
  2. 泛素-蛋白酶体途径在调节细胞周期蛋白依赖性激酶抑制剂 p27 的丰度中的作用。  |  Pagano, M., et al. 1995. Science. 269: 682-5. PMID: 7624798
  3. 泛素与细胞内蛋白质降解之谜。  |  Jennissen, HP. 1995. Eur J Biochem. 231: 1-30. PMID: 7628459
  4. 平衡, 发育和疾病中的泛素。  |  Muller, S. and Schwartz, LM. 1995. Bioessays. 17: 677-84. PMID: 7661849
  5. 泛素-蛋白酶体蛋白水解途径。  |  Ciechanover, A. 1994. Cell. 79: 13-21. PMID: 7923371
  6. 泛素介导的蛋白水解途径：识别蛋白水解底物和参与降解原生细胞蛋白的机制。  |  Ciechanover, A. and Schwartz, AL. 1994. FASEB J. 8: 182-91. PMID: 8119489
  7. 蛋白质降解还是调节：Ub the judge.  |  Hochstrasser, M. 1996. Cell. 84: 813-5. PMID: 8601303
  8. 通过一种新型泛素依赖性蛋白激酶活性对 IkappaBalpha 进行特定位点磷酸化。  |  Chen, ZJ., et al. 1996. Cell. 84: 853-62. PMID: 8601309

订购信息

产品名称产品编号规格价格数量收藏夹

Ubiquitin 抗体 (P4D1)

sc-8017
200 µg/ml
RMB2377.00

Ubiquitin (P4D1): m-IgG Fc BP-HRP 套装

sc-526508
200 µg Ab; 10 µg BP
RMB2662.00

Ubiquitin (P4D1): m-IgG1 BP-HRP 套装

sc-531881
200 µg Ab; 20 µg BP
RMB2662.00

Ubiquitin 抗体 (P4D1) AC

sc-8017 AC
500 µg/ml, 25% agarose
RMB3129.00

Ubiquitin 抗体 (P4D1) HRP

sc-8017 HRP
200 µg/ml
RMB2377.00

Ubiquitin 抗体 (P4D1) FITC

sc-8017 FITC
200 µg/ml
RMB2482.00

Ubiquitin 抗体 (P4D1) PE

sc-8017 PE
200 µg/ml
RMB2580.00

Ubiquitin 抗体 (P4D1) Alexa Fluor® 488

sc-8017 AF488
200 µg/ml
RMB2685.00

Ubiquitin 抗体 (P4D1) Alexa Fluor® 546

sc-8017 AF546
200 µg/ml
RMB2685.00

Ubiquitin 抗体 (P4D1) Alexa Fluor® 594

sc-8017 AF594
200 µg/ml
RMB2685.00

Ubiquitin 抗体 (P4D1) Alexa Fluor® 647

sc-8017 AF647
200 µg/ml
RMB2685.00

Ubiquitin 抗体 (P4D1) Alexa Fluor® 680

sc-8017 AF680
200 µg/ml
RMB2685.00

Ubiquitin 抗体 (P4D1) Alexa Fluor® 790

sc-8017 AF790
200 µg/ml
RMB2685.00

Ubiquitin 抗体 (P4D1) B

sc-8017 B
200 µg/ml
RMB2407.00

Ubiquitin 抗体 (P4D1) Alexa Fluor® 405

sc-8017 AF405
200 µg/ml
RMB2685.00

How do the antibody for IP?

Asked by: hahah
Thank you very much for your question.Ubiquitin Antibody (P4D1) is recommended for detection of Ubiquitin, poly-ubiquitinated and ubiquitinated proteins of mouse, rat, human and Drosophila origin by WB, IP, IF, IHC(P), FCM and ELISA.Warranty service is available. Thank you.
Answered by: Jiajun Z
Date published: 2025-03-31

我做GPX4的泛素化，请问免疫沉淀GPX4后，用Ub孵育的浓度是多少？我用1：200的，在180kda处有很明显条带，整个条带也没有拖尾现象。

Asked by: peitian
Thank you for your inquiry. For Western Blotting application，we suggest starting dilution 1:100, dilution range 1:100-1:1000. For more information please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。对于蛋白免疫印迹实验，我们建议起始稀释比例1:100，稀释范围是1:100-1:1000。更多信息请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Yanru Tao
Date published: 2024-10-29

will lactacystin always increase the ubiquitin staining in all the cells, as you have posted a figure in the datasheet about HeLA cells?

Asked by: shomaila
Thank you for your question. For assistance please contact our technical service department by emailing [email protected] or phone at (800)-457-3801.
Answered by: BlakeJ
Date published: 2024-06-12

We want to use P4D1 antibodies for Immunohistochemistry (IHC), Can you please recommend us some published papers where they have used these antibodies for IHC?

Asked by: Khushi_27
Thank you for your question. Here are some pub med ID numbers. 12145308 14739285 15220483 14657501
Answered by: BlakeJ
Date published: 2024-03-28

How good is this antibody in detecting mono-ubiquitinated protein in an immunoblot?

Asked by: Roshika R
We have several citations for this antibody for this assay . To request a trial size to test on your samples please contact technical service by emailing [email protected].
Answered by: BlakeJ
Date published: 2024-03-28

hi, I want to use Ubiquitin (P4D1) antibody for western, what concentration do you suggest? Thanks

Asked by: shomaila
Thank you for your question. We recommend 1:100-1:1000. https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2023-12-29

How good is this antibody for immunostaining?

Asked by: GB23
Thank you for your question. For assistance please contact our Technical service at (800)-457-3801.
Answered by: BlakeJ
Date published: 2022-09-02

这个抗体的分子量是确定的9KD吗？IP过的蛋白敷完这个抗体也是在9KD有条带吗？

Asked by: diandianshiwode
Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-11-28
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Rated 5 out of 5 by from Good for WBA clear band are detected in several cell lines and mouse tissue samples.
Date published: 2024-03-27
Rated 5 out of 5 by from Love it!Bought this im super happy i gave this app a chance
Date published: 2024-01-09
Rated 5 out of 5 by from well function in Western blotWhen using this antibody for WB experiments, it was very well to use, and I got the desired results the first time.
Date published: 2023-12-28
Rated 5 out of 5 by from It's effective, it's specificIt's effective, it's specific, it's used for immunofluorescence,
Date published: 2023-05-30
Rated 4 out of 5 by from Ubiquitin antibody for Western blotThe ubiquitin antibody works well for detection of ubiquitin in mouse brain and cell lysates. No background problems.
Date published: 2023-01-27
Rated 5 out of 5 by from very beautiful for WBI used it for WB, rat-cardiomyoblast, H9c2 cells. 1:500.
Date published: 2022-06-01
Rated 5 out of 5 by from good performanceThis antibody works well in Western blotting in our beta-catenin IP experiment
Date published: 2022-05-27
Rated 5 out of 5 by from Excellent functionI bought this product. It's very easy to use. I recommend you to buy this product.
Date published: 2022-04-29
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Ubiquitin 抗体 (P4D1) is rated 4.7 out of 5 by 100.
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